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Analysis on the molecular mechanism of the accumulation of seed storage materials and molecular breeding of phosphate storage

Research Project

Project/Area Number 13660004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

YOSHIDA Kaoru  Grad. Sch. Agri. Life Sci., Associate Professor, 大学院・農学生命科学研究科, 助教授 (70183994)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsRice / myo-inositol-1 -phosohate synthase / Phosphate / Seed storage products / Phytin / Molecular breeding / Phytoremediation / Gluterin / インシトール1リン酸合成酵素 / 貯蔵物質 / 形質転換 / GUS
Research Abstract

Phosphates in seeds are stored as phytic acid (myo-inositol -1,2,3,4,5,6-hexakisphosphate). Phytic acid is not readily digested by monogastric animals, so that grains and legumes in feed result in a large amount of phosphorus in animal wastes. Reducing the phytic acid content of seeds is a major objective of breeding, both to increase the available nutrients and to reduce the environmental load. Myo-inositol 3-phosphate synthase (EC 5.5.1.4) catalyzes the first step of phytic acid biosynthesis. We isolated that gene from rice (RINO1), and demonstrated that it plays a key role in the synthetic pathway during seed development. In this study, we attempted to reduce the phytic acid content of grain by reducing RINO1 expression in developing rice seeds.
To repress RINO1 expression, we introduced RINO1 cDNA in sense and antisense orientations under control of either the CaMV 35S, RINO1, or rice major storage protein glutelin (GluB-1) promoters, or the promoter of a transcriptional factor (RISBZ1) responsible for GluB-1 expression, into rice plants, using Agrobacterium tumefaciens-mediated transformation. To determine the effect of the transgene on phytic acid biosynthesis in seeds, the inorganic (Pi) and total phosphorus contents of the grain were examined. In non-transgenic rice seeds, the concentration of Pi is around 5% of the total phosphorus and the majority of the remaining phosphorus is phytic acid phosphorus. Several transgenic plants contained elevated Pi levels in T_1 seeds, varying from 12 to 63% Pi. The total phosphorus content of the seeds of transgenic plants remained constant, and equaled that of non-transgenic plants. The results suggest that the phytic acid content was reduced in these transgenic plants.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Yoshida, KT et al.: "The synergistic effects of sugar and abscisic acid on myo-inositol-1-phosphate synthase expression"Physiol. Plant.. 114. 581-587 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kuboyama T.et al.: "Antiserum against a stigma-exudate protein of tobacco, SE32,which was identical with PPAL, a beta-expansin-like protein specific to stigma, cross-reacted with another stigma-exudate protein, SE35"Breed. Sci.. 51. 131-135 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshida KT et al.: "A subtilisin-like serine protease specifically expressed in reproductive organs in rice"Sex. Plant Reprod.. 13. 193-199 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 吉田 薫: "遺伝子組換え植物の現状と育種学研究のこれから"育種学研究. 3. 230-232 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshida K.T. et al.: "The synergistic effects of sugar and abscisic acid on myo-inositol-1-phosphate synthase expression"Physiol. Plant.. 114. 581-587 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshida, K.T. and Kuboyama, T.: "A subtilisin-like serine profease specifically expressed in reproductive organs in rice"Sex. Plant Reprod.. 193-199 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kuboyama T. et al.: "Antiserum against a stigma-exudate protein of tobacco, SE32, which was identical with PPAL, a beta-expansin-like protein specific to stigma, cross-reacted with another stigma-exudate protein, SE35"Breed. Sci.. 51. 131-135 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshida K.T.et al.: "The synergistic effects of sugar and abscisic acid on myo-inositol-1-phosphate synthase expression"Physiol.Plant.. 114. 581-587 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshida K.T. et al.: "A subtilisin-like serine protease specifically expressed in reproductive organs in rice"Sex. Plant Reprod.. 13. 193-199 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuboyama, T. et al.: "Antiserum against a stigma-exudate protein of tobacco, SE32, which was identical with PPAL, a β-expansin-like protein specific to stigma, cross-reacted with another stigma-exudate protein, SE35"Breed. Sci.. 51. 131-135 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshida K.T. et al.: "The synergistic effects of sugar and abscisic acid on myo-inositol-1-phosphate synthase expression"Physiol. Plant.. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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