| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Research Abstract |
<Flower color and pigments in Primula kisoana var. shikokiana (PKS) and the related species> Flower colors of PKS were divided broadly into the categories of white, red and pinkish-purple. As a result of analysis by HPLC, 3 types of malvidin(M1,M2,M3) and 1 type of flavonol(F1) were detected from those corolla lobes, and red flowers had M2, M3 and small quantity of F1, pinkish-purple flowers had M1 and F1. Because of b^*/a^* were decreased according as the increase of F1/M1, mechanism of flower color variation in the pinkish-purple flowers were considered that originated from co-pigmentation, also the related species such as P. sieboldii.
<Polyploidy in PKS> 4n plant and 3n plants were obtained from F_1 plants of 2n x 2n and 3n x 2n, each. And also, 4n plants were obtained by colchicine treatment to adventitious buds on root segments, efficiently.
<Irrigation and culture methods in PKS > Drip-irrigation and Hammock culture method were good to culture, moreover root elongation and adventitious buds formation on roots were facilitated.
<Melastoina tetramerum Hayata> M. tetramerum has little merit compared with cultivars, but, we think that it is possible to improve horticultural value of this species by interspecific cross and to investigate environmental condition in culture, especially light condition
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