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Analysis of molecular interactions between a novel bromovirus and Arabidopsis thaliana

Research Project

Project/Area Number 13660049
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MISE Kazuyuki  Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (90209776)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsPlant virus / Bromovirus / Arabidopsis thaliana / Full-length cDNA / Mutant / Cell-to-cell movement / Symptom / Chromosome / ウイルス / 全身感染 / in vitro転写 / Tissue-printing
Research Abstract

Brome mosaic virus (BMV) is one of the most-studied plant virues, but does not infect model plants such as Arabidopsis thaliana. We have recently found that a related bromovirus, Spring beauty latnet virus (SBLV) systemically and efficiently infects A. thaliana. To establish a system for the anlysis of molecular interactions between SBLV and A. thaliana, we first constructed biologically active cDNA clones of SBLV genomic RNAs and determined their complete nucleotide sequences. To identify plant factor(s) involved in SBLV infection, (1) We inoculated 2,231 M2 (Col-0 background) plants mutagenized with EMS treament and identified one mutart line in which SBLV infection was delayed The SBLV infection in this line was shownto be affected at the step of the cell-to-cell movement Genetic analysis demonstrated that slower SBLVspread in this line was recessive. (2) We inoculated 67 Arabidopsis accesions with SBLV, and found anaccession, Pla-O in which SBLV spread was delayed at least in incculated leaves. The SBLV infection in Pla-0 was shown to be affected At the step of the cell-to-cell raovement rather than maltiplication within a single (cell.Genetic analysis demonstrated that slower SBLV spread in Pla-0 was recessive or semidominant trait. (3) Symptom development was examined in A. thaliana accenssions infected with SBLV. Of 85 Arabidopsis accessions tested, four accessions including an accession S96 were Scnsitive to SBLV infection, while the otner accessions including Col-0 were symptomless or developed only mild symptoms. Genetic study suggested that the symptom phenotype of S96 is controlled by a single semidominant lolcus. We bave designated this locus SSBI. Infectivity of SBLV in plants and protoplasts were compalable between Col-0and S96, indicating that variation of symptom phenotype was not due to the different infectivity of SBLV By using genetic markers, SSBI was mapped between ATMB3R and JM142 on chromosome IV.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Sasaki, N.: "Site-specific single amino acid changes to Lys or Arg in the central region of the movement protein of a hybrid bromovirus are required for adaptation to a nonhost"Virology. 279(1). 47-57 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okinaka, Y.: "The C terminus of Brome mosaic virus coat protein controls viral cell-to-cell and long-distance movement"Journal of Virology. 75(11). 5385-5390 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mori, M.: "Inducible high-level mRNA amplification system by viral replicase in transgenic plants"Plant Journal. 27(1). 79-88 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagano, H.: "Conversion in the requirement of coat protein in cell-to-cell movement mediated by the cucumber mosaic virus movement protein"Journal of Virology. 75(17). 8045-8053 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Dohi, K.: "Brome mosaic virus replicase proteins localize with the movement protein at infection-specific cytoplasmic inclusions in infected barley leaf cells"Archives of Virology. 146(8). 1607-0615 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Damayanti, T.A.: "Positional effect of deletions on viability, especially on encapsidation, of Brome mosaic virus D-RNA in barley protoplasts"Virology. 293(2). 314-319 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Dohi, K.: "RNA-dependent RNA polymerase complex of Brome mosaic virus : analysis of the molecular structure with monoclonal antibodies"Journal of General Virology. 83(11). 2879-2890 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeda, A.: "Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus"FEBS Letters. 532(1-2). 75-79 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagano, H.: "Plant virus cell-to-cell movement mediated by the cucumber mosaic virus movement protein"Recent Research Development in Virology. 4. 99-105 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 三瀬和之: "植物ウイルス外被タンパク質の多機能性-ゲノム核酸の保護だけでなく,細胞間移行,長距離移行など感染の様々なステップに関わることが明らかに"化学と生物. 40(6). 354-356 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujisaki, K.: "Complete nucleotide sequence of spring beauty latent virus, a bromovirus infectious to Arabidopsis thaliana"Archives of Virology. 148(1). 165-175 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, N.: "The movement protein gene is involved in the virus -specific requirement of the coat protein in cell-to-cell movement of bromoviruses"Archives of Virology. 148(4). 803-812 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okinaka, Y.: "Characterization of a novel barley protein, HCP1, that interacts with the Brome mosaic virus coat protein"Molecular Plant-Microbe Interactions. 16(4). 352-359 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujisaki, K.: "Use of Spring beauty latent virus to identify compatible interactions between bromovirus components required for virus infection"Journal of General Virology. 84(6). 1367-1375 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, N.: "Site-specific single amino acid changes to Lys or Arg in the central region of the movement protein of a hybrid bromovirus are required for adaptation to a nonhost"Virology. 279(1). 47-57 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okinaka, Y.: "The C terminus of Brome mosaic virus coat protein controls viral cell-to-cell and long-distance movement"J. Virol. 75(11). 5385-5390 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mori, M.: "Inducible high-level mRNA amplifucation system by viral replicase in transgenic plants"Plant J.. 27(1). 79-88 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagano, H.: "Conversion in the requirement of coat protein in cell-to-cell movement mediated by the cucumber mosaic virus movement Protein"J. Virol.. 75(17). 8045-8053 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Dohi, K.: "Brome mosaic virus replicase proteins localize with the movement protein at infection-specific cytoplasmic inclusions in infected barley leaf cells"Arch. Virol. 146(8). 1607-1615 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Damayanti, T. A.: "Positional effect of deletions on viability, especially on encapsidation, of Brome mosaic virus D-RNA in barley protoplasts"Virology. 293(2). 314-319 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Dohi, K.: "RNA-dependent RNA polymerase complex of Brome mosaic virus: analysis of the molecular Structure with monoclonal antibodies"J. Gen. Virol. 83(11). 2879-2890 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeda, A.: "Identification of a novel RNA silencing suppressor, Nssprotein of Tomato spotted wilt virus"FEBS Lett.. 532(1-2). 75-79 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagano, H.: "Plant virus cell-to-cell movement mediated by the cucumber mosaic virus movement protein"Recent Res. Dev. Virol.. 4. 99-105 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mise, K.: "Multifunctional capsid proteins of plant viruses"Chemistry and Biology. 40(6). 354-356 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujisaki, K.: "Complete nucleotide sequence of spring beauty latent virus, aromovirus infectious to Arabidopsis thaliana"Arch. Virol.. 148(1). 165-175 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, N.: "The movement protein gene is involved in the virus- specific requirement of the coat protein in cell-to-cell movement of bromoviruses"Arch. Virol.. 148(4). 803-812 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okinaka, Y.: "Characterization of a novel barley protein, HCP1, that interacts with the Brome mosaic virus coat protein"Mol. Plant-Microbe. Interact.. 16(4). 352-359 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujisaki, K: "Use of Spring beauty Latent virus to identify compatible interactions between bromovirus components required for virus infection"J. Gen. Virol.. 84(6). 1367-1375 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Damayanti, T.A.: "Positional effect of deletions on viability, especially on encapsidation, of Brome mosaic virus D-RNA in barlegy protop lasts"Virology. 293(2). 314-319 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Dohi, K.: "RNA-dependent RNA polymerase complex of Brome mosaic virus : analysis of the molecular structure with monoclonal antibodies"Journal of General Virology. 83(11). 2879-2890 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeda, A.: "Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus"FEBS Letters. 532 (1-2). 75-79 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagano, H.: "Plant virus cell-to-cell movement mediated by the cucumber mosaic virus movement protein"Recent Research Development in Virology. 4. 99-105 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 三瀬和之: "植物ウイルス外被タンパク質の多機能性 -ゲノム核酸の保護だけでなく,細胞間移行,長距離移行など感染の様々なステップに関わることが明らかに"化学と生物. 40(6). 354-356 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujisaki, K.: "Complete nucleotide sequence of spring beauty latent virus, a bromovirus infectious to Arabidopsis thaliana"Archives of Virology. 148(1). 165-175 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sasaki, N.: "The movement protein gene is involved in the virus-specific requirement of the coat protein in cell-to-cell movement of bromoviruses"Archives of Virology. 148(4). 803-812 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okinaka, Y.: "Characterization of a novel barley protein, HCP1, that interacts with the Brome mosaic virus coat protein"Molecular Plant-Microbe Interactions. 16(In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujisaki, K.: "Use of Spring beauty latent virus to identify compatible interactions between bromovirus components required for virus infection"Journal of General Virology. 84(In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sasaki, N.: "Site-specific single amino acid changes to Lys or Arg in the central region of the movement protein of a hybrid bromovirus are required for adaptation to a nonhost"Virology. 279(1). 47-57 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Okinaka, Y.: "The C terminus of Brome mosaic virus coat protein controls viral cell-to-cell and long-distance movement"J. Virol.. 75(11). 5385-5390 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mori, M.: "Inducible high-level mRNA amplification system by viral replicase in transgenic plants"Plant J.. 27(1). 79-88 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagano, H.: "Conversion in the requirement of coat protein in cell-to-cell movement mediated by the cucumber mosaic virus movement protein"J. Virol.. 75(17). 8045-8053 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Dohi, K.: "Brome mosaic virus replicase proteins localize with the movement protein at infection-specific cytoplasmic inclusions in infected barley leaf cells"Arch. Virol.. 146(8). 1607-1615 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Damayanti, T.A.: "Positional effect of deletions on viability, especially on encapsidation, of Brome mosaic virus D-RNA in barley protoplasts"Virology. 293(2). 314-319 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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