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Immunoelectron microscopic analysis of callose secretion and nuclear condensation associated with defense responses of plant pathogen-stressed plant cells

Research Project

Project/Area Number 13660051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionGraduate School of Science and Technology, Kobe University

Principal Investigator

PARK Pyoyun  Kobe University, Graduate School of Science and Technology Professor, 自然科学研究科, 教授 (20147094)

Co-Investigator(Kenkyū-buntansha) MAYAMA Shigeyuki  Kobe University, Faculty of Agriculture Professor, 農学部, 教授 (00112251)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsAlternaria altenata Japanese pear pathotype / Host-specific toxin / AK-toxin / callose / papillae / β-1,3-D-glucan / Nuclear condensation / ナシ黒斑病菌 / AK毒素 / β-1,3-グルカン / β-1, 3-グルカン
Research Abstract

Spores of Alternaria alternata Japanese pear pathotype germinated and formed appressoria at tips of germ tubes on surfaces of the susceptible and resistant pear leaves. Penetration pegs emerged from appressorial bottom made an attempt to invade cell walls of susceptible epidermal cells. The pegs penetrated into the cuticular layers and then were grown as infection hyphae along the pectin layers, but didn't invade directly the epidermal cytoplasm except for the completely disrupted cells. AK-toxin-induced plasma membrane modifications, associated with callose secretion, appeared at plasmodesmata of susceptible leaf cells before the fungal invasion in tissues, suggesting the hypothesis that the toxin was produced from germ tubes was also supported. On the contrary, the pathogen inoculated in resistant leaves was able to penetrate into cuticles, but fungal invasion was not limited after the cuticular penetration. These results indicated that resistant mechanism to black spot disease was e … More xpressed at the stage of cuticular invasion during pathogenesis. Papilla formation was found at the spaces between cell walls and plasma membranes irrespective of susceptible and resistant leaves against pathogen inoculation, but penetration pegs were observed, but not involved in the papillae themselves. This suggests that papillae are non-specific plant responses against cuticular wounding, but didn't contribute directly to disease resistance in black spot disease. Immunoelectron microscopic study showed that β-l,3-D-glucan accumulated both at papillae in the pathogen-infected susceptible and resistant leaves, and at callose in the toxin-treated susceptible ones. The glucan was shown to be be secreted from Golgi bodies of susceptible leaves via Golgi vesicles to damaged sites of plasma membranes, plasma membrane modifications. The generation of reactive oxygen species (ROS) wsa found at the membrane fragments of plasma modifications in the toxin-treated susceptible leaves, but not at the papillae. This results showed that ROS generation was associated with the toxin-induced plasma membrane modifications which were damaged plasmalemma which were spatially consistent with cellular ion-leakage sites. ROS generation also was recognized at the cell walls of penetration pegs during infection process in the pathogen-inoculated susceptible and resistant leaves. ROS might cause hardening cell walls by cross-reaction of cell wall proteins with ROS, suggesting increased aggressiveness of penetration pegs. Although nuclear condensation was observed in the toxin-treated and spore-inoculated susceptible leaves, the nuclear changes were not evaluated as one of apoptotic events. The changes were compatible with pknosis seen in necrotic tissues because of no laddering of DNA. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Shinogi, T., Suzuki, T., Narusaka, Y., Park, P.: "Ultrastructural localization of hydrogen peroxide in host leaves treated with AK-toxin I produced by Alternaria alternata Japanese pear pathotype"Journal of General Plant Pathology. Vol.68. 38-45 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki, T., Shinogi, T., Unno, K., Narusaka, Y., Park, P.: "β-1,3,-glucan transported from Golgi apparatus of Japanese pear leaves is a component of extracellular polysaccharides accumulated after AK-toxin treatment"Journal of General Plant Pathology. Vol.68. 267-276 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinogi, T., Suzuki, T., Kurihara, T., Narusaka, Y., Park, P.: "Microscopic detection of reactive oxygen species generation in the compatible and incompatible interactions of Alternari alternata Japanese pear pathotype and host plants"Journal of General Plant Pathology. Vol.69. 7-16 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki, T., Shinogi, T., Narusaka, Y., Park, P.: "Infection behavior of Alternaria alternata Japanese pathotype and localization of 1,3,-β-D-glucan in compatible and incompatible interactions between the pathogen and host plants"Journal of General Plant Pathology. Vol.69. 91-100 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinogi,T., Suzuki, T., Narusaka,T., and Park,P.: "Ultrastructural localization of hydrogen peroxide in host leaves treated with AK-toxin I produced by Alternaria alternata Japanese pear pathotype"Journal of General Plant Pathology. 68. 38-45 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki,T., Shinogi,T., Unno,K., Narusaka,Y., and Park,P.: "β-1,3-D-glucan transported from Golgi apparatus of Japanese pear leaves is a component of extracellular polysaccharides accumulated after AK-toxin I treatment"Journal of General Plant Pathology. 68. 267-276 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinogi,T., Suzuki,T., Kurihara,T., Narusaka,T., and Park,P.: "Microscopic detection of reactive oxygen species generation in the compatible and incompatible interactions of Alternaria alternata Japanese pear pathotype and host plants"Journal of General Plant Pathology. 69. 7-16 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki,T., Shinogi,T., Narusaka,Y., and Park,P.: "Infection behavior of Alternaria alternata Japanese pear pathotype and localization of 1,3-β-D-glucan in compatible and incompatible interactions between the pathogen and host plants"Journal of General Plant Pathology. 69. 91-100 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinogi, T., Suzuki, T., Narusaka, Y., Park, P.: "Ultrastructural localization of hydrogen peroxide in host leaves treated with AK-toxin I produced by Alternaria alternata Japanese pear pathotyne"Journal of General Plant Pathology. Vol.68, No.1. 38-45 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shinogi, T., Suzuki, T., Kurihara, T., Narusaka, Y., Park, P.: "Microscopic detection of reactive oxygen species generation in the compatible and incompatible interactions of Alternaria alternata Japanese pear pathotype and host plants"Journal of General Plant Pathology. Vol.69, No.1. 7-16 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, T., Shinogi, T., Narusaka, Y., Park, P.: "Infection behavior of Alternaria alternata Japanese pear pathotype and localization of 1,3-β-D-glucan in compatible and incompatible interactions between the pathogen and host plants"Journal of General Plant Pathology. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, T., Shinogi, T., Unno, K., Narusaka, Y., Park, P.: "β-1,3-D-glucan transported from Golgi apparatus of Japanese pear leaves is a component of extracellular polysaccharides accumulated after AK-toxin treatment"Journal of General Plant Pathology. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shinogi, T., Suzuki, T., Narusaka, Y., Park, P.: "Ultrastructural localization of hydrogen peroxide in host leaves treated with AK-toxin I produced by Alternaria alternata Japanese pear pathotype"J.Gen.Plant Pathol.. 68(in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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