Creation of high nitric oxide spin-trapping molecule using hemepeptide
| Project/Area Number |
13660101
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Nihon University |
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Principal Investigator |
OKU Tadatake Nihon University, College of Bioresource Sciences, Professor, 生物資源科学部, 教授 (20059637)
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| Co-Investigator(Kenkyū-buntansha) |
KAWACHI Ryu Nihon University, College of Bioresource Sciences, Assistant, 生物資源科学部, 助手 (70339290)
NISHLO Toshiyuki Nihon University, College of Bioresource Sciences, Vice-professor, 生物資源科学部, 助教授 (10256836)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | hemepeptide / nitric oxide trapper / cytochrome / Porphyra yezoensis / horse heart muscle / Escherichia coli / expression / enzyme digestion / NOトラップ / 立体構造 / ペプチド精製 / NOトラップ能 / 物理化学的性質 / タンパク質水解 / NO捕捉 |
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| Research Abstract |
To develop the nitric oxide trapper using hemepeptide, miniature molecules containing heme were prepared by enzymatic hydrolysis of commercially equine heart cytochrome c and by cloning and expression of the red alga Porphyra yezoensis cytochrome c in E. coli, in which histidine is axially coordinated to heme iron, and acts as its fifth ligand.
The nitric oxide trapping activity of the shortest nonapeptide (9 amino acid residues) was approximately 120-fold that of equine heart cytochrome c (104 residues), compared with other peptides (22, 32, 57 and 66 residues). From results, it was assumed that the shorter molecule in prepared hemepeptides, which were water-soluble hemepeptide, showed the higher NO trapping activity, because the collision probability of the peptide chain. The shortest nonapeptide (9 residue) may be a novel molecule as the trapper of NO in an aqueous solution.
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Report
(4 results)
Research Products
(8 results)
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[Publications] Tadashi Satoh, Akito Itoga, Yasuhiro Isogai, Masaaki Kurihara, Seiji Yamada, Miwa Natori, Noriko Suzuki, Kohei Suruga, Ryu Kawachi, Masaomi Arahira, Toshiyuki Nishio, Chikahusa Hukazawa, Tadatake Oku: "Increasing stability by replacement of heme axial ligand in c-type cytochrome"FEBS Letters. 531. 543-547 (2002)
Description
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[Publications] Kohei Suruga, Kiriko Murakami, Yohei Taniyama, Toshiyuki Hama, Hirotaka Chida, Tadashi Satoh, Seiji Yamada, Wataru Hakamata, Ryu Kawachi, Toshiyuki Nishio, Tadatake Oku: "A novel microperoxidase activity: methyl viologen-linked niterite reducing activity of microperoxidase"Biochemical and Biophysical Research Communications. 315. 815-822 (2004)
Description
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Related Report
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