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Creation of high nitric oxide spin-trapping molecule using hemepeptide

Research Project

Project/Area Number 13660101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionNihon University

Principal Investigator

OKU Tadatake  Nihon University, College of Bioresource Sciences, Professor, 生物資源科学部, 教授 (20059637)

Co-Investigator(Kenkyū-buntansha) KAWACHI Ryu  Nihon University, College of Bioresource Sciences, Assistant, 生物資源科学部, 助手 (70339290)
NISHLO Toshiyuki  Nihon University, College of Bioresource Sciences, Vice-professor, 生物資源科学部, 助教授 (10256836)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordshemepeptide / nitric oxide trapper / cytochrome / Porphyra yezoensis / horse heart muscle / Escherichia coli / expression / enzyme digestion / NOトラップ / 立体構造 / ペプチド精製 / NOトラップ能 / 物理化学的性質 / タンパク質水解 / NO捕捉
Research Abstract

To develop the nitric oxide trapper using hemepeptide, miniature molecules containing heme were prepared by enzymatic hydrolysis of commercially equine heart cytochrome c and by cloning and expression of the red alga Porphyra yezoensis cytochrome c in E. coli, in which histidine is axially coordinated to heme iron, and acts as its fifth ligand.
The nitric oxide trapping activity of the shortest nonapeptide (9 amino acid residues) was approximately 120-fold that of equine heart cytochrome c (104 residues), compared with other peptides (22, 32, 57 and 66 residues). From results, it was assumed that the shorter molecule in prepared hemepeptides, which were water-soluble hemepeptide, showed the higher NO trapping activity, because the collision probability of the peptide chain. The shortest nonapeptide (9 residue) may be a novel molecule as the trapper of NO in an aqueous solution.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Tadatake Oku, et al.: "Increasing stability by replacement of heme axial ligand in c-type cytochrome"FEBS Letters. 531. 543-547 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tadatake Oku, et al.: "A novel microperoxidase activity : methyl viologen-linked niterite reducing activity of microperoxidase"Biochemical and Biophysical Research Communications. 315. 815-822 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tadashi Satoh, Akito Itoga, Yasuhiro Isogai, Masaaki Kurihara, Seiji Yamada, Miwa Natori, Noriko Suzuki, Kohei Suruga, Ryu Kawachi, Masaomi Arahira, Toshiyuki Nishio, Chikahusa Hukazawa, Tadatake Oku: "Increasing stability by replacement of heme axial ligand in c-type cytochrome"FEBS Letters. 531. 543-547 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohei Suruga, Kiriko Murakami, Yohei Taniyama, Toshiyuki Hama, Hirotaka Chida, Tadashi Satoh, Seiji Yamada, Wataru Hakamata, Ryu Kawachi, Toshiyuki Nishio, Tadatake Oku: "A novel microperoxidase activity: methyl viologen-linked niterite reducing activity of microperoxidase"Biochemical and Biophysical Research Communications. 315. 815-822 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oku, T., et al.: "A novel microperoxidase activity : methyl viologen-linked nitrite reducing activity of microperoxidase"Biochem.Biophys.Res.Commun.. 315. 815-822 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Oku, T., et al.: "Radiation-Induced Nitrite Reducing Activity of Cytochrome c"J.Agric.Food Chem.. 51(23). 6835-6843 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Oku, T., et al.: "Increasing the conformational stability by replacement of heme axial ligand in c-type cytochrome"FEBS Letters. 531(11). 543-547 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oku, T., et al.: "Appearance of Nitrite Reducing Activity of Cytochrome c upon Heat Denaturation"Biosci. Biotechnol. Biochem.. 66(10). 2044-2051 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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