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Development of novel nutritional methods to inhibit muscle atrophy caused by microgravity.

Research Project

Project/Area Number 13660126
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionThe University of Tokushima

Principal Investigator

KISHI Kyoichi  The University of Tokushima, School of Medicine, Professor, 医学部, 教授 (80035435)

Co-Investigator(Kenkyū-buntansha) ROKUTAN Kazuhito  The University of Tokushima, School of Medicine, Associate Professor, 医学部, 助教授 (10230898)
NIKAWA Takeshi  The University of Tokushima, School of Medicine, Assistant Professor, 医学部, 助手 (20263824)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsmicrogravity / DNA microarray / cysteine / vitamin E / ubiquitin
Research Abstract

We have previously reported that spaceflight and tail suspension enhanced degradation of rat myosin heavy chain (MHC) in association with activation of a ubiquitin-dependent proteolytic pathway. In the serial study, about 26,000 gastrocnemius muscle gene expression in rats exposed to the spaceflight or tail-suspension was accessed by DNA microarray analysis, and we found that gene expression in the ubiquitin-dependent proteolysis in these rats was up-regulated. In the present study, to elucidate whether the altered gene expression is accompanied by oxidative stress, we measured markers for oxidative stress, such as thiobarbituric acid ?reactive substance (TBARS), glutathione disulfide (GSSG), and glutathione (GSH), in gastrocnemius muscle of tail-suspended rat. Tail-suspension reciprocally increased concentration of TBARS and GSSG in parallel with enhancement of protein ubiquitination, suggesting that oxidative stress may play an important role in protein ubiquitination caused by tail-suspension. To prevent ubiquitination associated with oxidative stress, we also administered an antioxidative nutrient, cysteine and vitamin E, to tail-suspension rats. Intragastric supplementation of 140 mg/rat of cysteine for 2 weeks or longer normalized the ratio of GSH to GSSG in the muscle and suppressed protein ubiquitination and MHC fragmentation, compared with supplementation of the equimolar amount of alanine. The cysteine supplementation significantly suppressed the loss of hindlimb muscle mass. Not only supplementation of 15 mg/rat of α-tocopherol did not suppressed protein ubiquitination and MHC fragmentation, but also loss of hindlimb muscle mass. Our results suggest that effect of antioxidative nutrients depend on supplement amount and may be beneficial for preventing ubiquitination of muscle proteins caused by unweighting.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Ikemoto M et al.: "Cysteine supplementation prevents unweighting-induced ubiquitination in association with redox regulation in rat skeletal muscle"Biol.Chem.. 383(3-4). 715-721 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nikawa T et al.: "Effects of soy protein diet on exercise-induced muscle protein catabolism in rats"Nutrition. 18(6). 490-495 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikemoto M et al.: "A relative high dose of vitamin E does not attenuate unweighting-induced oxidative stress and ubiquitination in rat skeletal muscle"J.Phys.Anthr.. 21(5). 257-263 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nikawa T et al.: "Impaired vitamin A-mediated mucosal IgA response in IL-5 receptor-knockout mice"Biochem.Biophys.Res.Commun.. 285(2). 546-549 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikemoto M et al.: "Space shuffle flight (STS-90) enhances degradation of rat myosin heavy chain in association with activation of ubiquitin-proteasome pathway"FASEB J.. 15(7). 1279-1281 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nikawa T et al.: "Interleukin-1β enhances retinoic acid-mediated expression of bone-type alkaline phosphatase in rat IEC-6 cells"Am.J.Physiol.Gastrointest.Liver Physiol.. 280(3). G510-G517 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 岸 恭一: "アミノ酸の生理作用:とくにシステイン"臨床栄養:アミノ酸栄養の最近の話題. 5 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kano M, Kitano T, Ikemoto M, Hirasaka K, Asanoma Y, Ogawa T, Takeda S, Nonaka I, Adams GR, Baldwin KM, Oarada M, Kishi K, Nikawa T: "Isolation and characterization of a novel gene Sfig in rat skeletal muscle up-regulated by spaceflight (STS-90)"J Med Invest. 50(1-2). 39-47 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikemoto M, Okamura Y, Kano M, Hirasaka K, Tanaka R, Yamamoto T, Ogawa T, Sairyo K, Kishi K, Nikawa T: "A relative high dose of vitamin E does not attenuate unweighting-induced oxidative stress and ubiquitination in rat skeletal muscle"J Phys Anthr. 21(5). 257-263 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nikawa T, Ikemoto M, Sakai T, Kano M, Kitano T, Kawahara T, Teshima S, Rokutan K, Kishi K: "Effects of soy protein diet on exercise-induced muscle protein catabolism in rats."Nutrition. 18(6). 490-495 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikemoto M, Nikawa T, Kano M, Hirasaka K, Kitano T, Watanabe C, Tanaka R, Yamamoto T, Kishi K: "Cysteine supplementation prevents unweighting-induced ubiquitination in association with redox regulation in rat skeletal muscle."Biol Chem. 383(3-4). 715-721 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nikawa T, Ikemoto M, Watanabe C, Kitano T, Kano M, Yoshimoto M, Towatari T, Katunuma N, Shizuka F, Kishi K: "A cysteine protease inhibitor prevents suspension-induced declines in bone weight and strength in rats."J Phys Anthr. 21(1). 51-57 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawahara T, Kuwano Y, Teshima-Kondo S, Kawai T, Nikawa T, Kishi K, Rokutan K: "Toll-like receptor 4 regulates gastric pit cell responses to Helicobacter pylori infection"J Med Invest. 48(3-4). 190-197 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawahara T, Kuwano Y, Teshima-Kondo S, Sugiyama T, Kawai T, Nikawa T, Kishi K, Rokutan K: "Holicobacter pylori lipopolysaccharide from type I, but not type II strains, stimulates apoptosis of cultured gastric mucosal cells."J Med Invest. 48(3-4). 167-174 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nikawa T, Ikemoto M, Kano M, Tokuoka K, Hirasaka K, Uehara S, Takatsu K, Rokutan K, Kishi K: "Impaired vitamin A-mediated mucosal IgA response in IL-5 receptor-knockout mice."Biochem Biophys Res Commun. 285(2). 546-549 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikemoto M, Nikawa T, Takeda S, Watanabe C, Kitano T, Baldwin KM, Izumi R, Nonaka I, Towatari T, Teshima S, Rokutan K, Kishi K: "Space shuttle flight (STS-90) enhances degradation of rat myosin heavy chain in association with activation of ubiquitin-proteasome pathway."FASEB J. 15(7). 1279-1281 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nikawa T, Ikemoto M, Tokuoka K, Teshima S. Alpers DH, Masui Y, Kishi K, Rokutan K: "Interleukin-1 beta enhances retinoic acid-mediated expression of bone-type alkaline phosphatase in rat IEC-6 cells."Am J Physiol Gastrointest Liver Physiol. 280(3). G510-G517 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikemoto M et al.: "Cysteine supplementation prevents unweighting-induced ubiquitination in association with redox regulation in rat skeletal muscle"Biol. Chem.. 383(3-4). 715-721 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nikawa T et al.: "Effects of soy protein diet on exercise-induced muscle protein catabolism in rats"Nutrition. 18(6). 490-495 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikemoto M et al.: "A relative high dose of vitamin E does not attenuate unweighting-induced oxidative stress and ubiquitination in rat skeletal muscle"J. Phys. Anthr.. 21(5). 257-263 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nikawa T et al.: "Impaired vitamin A-mediated mucosal IgA rcesponse in IL-5 receptor-knockout mice"Biochem. Biophys. Res. Commun.. 285(2). 546-549 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikemoto M et al.: "Space shuttle flight (STS-90) enhances degradation of rat myosin heavy chain in association with activation of ubiquitin-proteasome pathway"FASEB J.. 15(7). 1279-1281 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nikawa T et al.: "Interleukin-1β enhances retinoic acid-mediated expression of bone-type alkaline phosphatase in rat IEC-6 cells"Am. J. Physiol. Gastrointest. Liver Physiol.. 280(3). G510-G517 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 岸 恭一: "アミノ酸の生理作用:とくにシステイン"臨床栄養:アミノ酸栄養の最近の話題. 5 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikemoto M et al.: "Cysteine supplementation prevents unweighting-induced ubiquitination in association with redox regulation in rat skeletal muscle"Biol.Chem.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nikawa T et al.: "Effects of Soy Protein Diet on Exercise-Induced Muscle Protein Catabolism in Rats"Nutrition. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kawahara T et al.: "Toll-like receptor 4 regulates gastric pit cell responses to Helicobacter pylori infection"J.Med.Invest.. 48(3-4). 190-197 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nikawa T et al.: "Impaired vitamin A-mediated mucosal IgA response in IL-5 receptor-knockout mice"Biochem.Biophys.Res.Commun.. 285(2). 546-549 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ikemoto M et al.: "Space shuttle flight(STS-90)enhances degradation of rat myosin heavy chain in association with activation of ubiquitin-proteasome pathway"FASEB J.. 15(7). 1279-1281 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nikawa T et al.: "Interleukin-1β enhances retinoic acid-mediated expression of bone-type alkaline phosphatase in rat IEC-6 cells"Am.J.Physiol.Gastrointest.Liver Physiol.. 280(3). G510-G517 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 岸恭一, 六反一仁, 二川健: "アミノ酸の生理作用:アミノ酸の生理作用:とくにシステイン"臨床栄養:アミノ酸栄養の最近の話題. 5 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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