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Molecular mechanism of cancer chemoprevention by anthocyanins

Research Project

Project/Area Number 13660130
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionKagoshima University

Principal Investigator

HOU De-xing  Kagoshima University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (90305160)

Co-Investigator(Kenkyū-buntansha) FUJII Makoto  Kagoshima University, Faculty of Agriculture, Professor, 農学部, 教授 (70041655)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsAnthocyanins / Functional food / molecular nutrition / Apoptosis / Reactive oxygen species / JNK / c-jun / Caspases / アポートシス / 抗癌プロモーション作用 / MAPキナーゼ / AP-1活性 / ブルーベリー / 紫イモ
Research Abstract

Anthocyanidins are the aglycon nucleuses of anthocyanins, which are reddish pigments widely spread in colored fruits and vegetables. To investigate the anti-cancer effect of anthocyanins, induction of apoptosis was tested in human promyelocytic leukemia cells (HL-60), which is a valid model for testing antileukemic or general antitumoral compounds. Of six anthocyanidins representing the aglycons of most of anthocyanins, only those with an ortho-dihydroxyphenyl structure on the B-ring induce apoptosis, suggesting that the ortho-dihydroxyphenyl structure of anthocyanidins may contribute to the induction of apoptosis. Delphinidin, the most potent inducer, causes apoptosis in a time-and dose-dependent manner. The efficacious induction of apoptosis was observed at 100 μM for 6 h. Concomitant with the apoptosis, delphinidin stimulates JNK pathway activation including JNK phosphorylation and c-jun gene expression, and activates caspase-3. Antioxidants including N-acetyl-L-cysteine (NAC) and catalase effectively block delphinidin-induced JNK phosphorylation, caspase-3 activation, and DNA fragmentation. Moreover, anthocyanidins directly cause HL-60 cells to generate intracellular hydrogen peroxide. Thus, anthocyanidins may trigger an apoptotic death program through an oxidative stress-involved JNK signaling pathway. The induction of apoptosis by anthocyanins may be the pivotal mechanism by which its chemopreventive action against cancer is based.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Hou D-X. et al.: "Potential Mechanisms of cancer chemoprevention by anthocyanins (Review)."Current Mol.Med.. 3. 149-159 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hou D-X. et al.: "Anthocyanidins induce apoptosis in human promyelocytic leukemia cells : Structure-activity relationship and mechanisms involved"Int.J.Oncology. 23. 705-712 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hou D-X. et al.: "Anthocyanidins inhibit activator protein-1 activity and cell transformation : structure-Activity relationship and molecular mechanisms."Carcinogenesis. 25. 29-36 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Konczak-Islam, I. et al.: "Potential chemopreventive properties of anthocyanin-rich aqueous extracts from in vitro produced Tissue of sweetpotato (Ipomoeabatatas L.)."J.Agric.Food.Chem.. 51. 5916-5922 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hou D-X. et al.: "Potential Mechanisms of cancer chemoprevention by anthocyanins (Review)."Current Mol.Med.. 3. 149-159 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hou D-X. et al.: "Anthocyanidins induce apoptosis in human promyelocytic leukemia cells : Structure-activity relationship and mechanisms involved"Int.J.Oncology. 23. 705-712 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hou D-X. et al.: "Anthocyanidins inhibit activator protein-I activity and cell transformation : structure-Activity relationship and molecular mechanisms."Carcinogenesis.. 25. 29-36 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Konczak-Islam, I et al.: "Potential Chemopreventive Properties of Anthocyanin-rich Aqueous Extracts from in vitro Produced Tissue of Sweetpotato (Ipomoea batatas L.)."J.Agric.Food.Chem.. 51. 5916-5922 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hou D-X. et al.: "Potential Mechanisms of cancer chemoprevention by anthocyanins (Review)"Current Mol.Med.. 3. 149-159 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hou D-X. et al.: "Anthocyanidins induce apoptosis in human promyelocytic leukemia cells : Structure-activity relationship and mechanisms involved"Int.J.Oncology. 23. 705-712 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hou D-X. et al.: "Anthocyanidins inhibit activator protein-1 activity and cell transformation : structure-Activity relationship and molecular mechanisms"Carcinogenesis. 25. 29-36 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Konczak-Islam, I et al.: "Potential chemopreventive properties of anthocyanin-rich aqueous extracts from in vitro produced Tissue of sweetpotato (Ipomoea batatas L.)"J.Agric.Food.Chem.. 51. 5916-5922 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okada, M. et al.: "Myb controls G2/M progression by inducing cyclin B expression in the Drosophila eye imaginal disc"The EMBO Journal. 21. 675-684 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 侯 徳興: "がん予防能食品の検出システムの開発およびその応用"バイオインダストリー. 19. 33-37 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hou.D.-X.: "Potential mechanisms of cancer chemoprevention by anthocyanins (Review)"Current Molecular Medicine. 3. 149-159 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hou.D.-X.et al.: "Anthocyanidins induce apoptosis in human promyelocytic leukemia cells : structure-activity relationship and mechanisms involved"International Journal of Oncology. (印刷中). (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hou, D.-X., et al.: "Fisetin induces transcription of NADPH : quinone oxidoreductase gene through an antioxidant responsive element-involved activation"International Journal of Oncology. 18. 1175-1179 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hou, D.-X., et al.: "Expression of cell adhesion molecules and albumin genes in primary culture of rat hepatocytes"Cell Biology, International. 25. 239-244 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hou, D.-X., et al.: "Primary culture of chicken hepatocytes as an in vitro model for determining the influence of dioxin"Bioscience, Biotechnology, Biochemistry. 65. 218-221 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ushikai, M., Miyaji, Y., Kawabe, K., Hou, D.-X.: "Sequence of a quail calpain cDNA"Journal of Animal Science. 79. 775-776 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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