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Study on the interaction between mycorrhizal fungi and soil inbaiting mycoparasite

Research Project

Project/Area Number 13660159
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionShinshu University

Principal Investigator

OHMASA Masatake  Shinshu University, Faculty of Agriculture, Professor, 農学部, 教授 (60281036)

Co-Investigator(Kenkyū-buntansha) YAMADA Akiyoshi  Shinshu University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (10324237)
廣田 満  国立大学法人信州大学, 農学部, 教授 (90199133)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsMycorrhizal fungi / Trichoderma / fungal interaction / agar medium / cultivation condition / concentration of sugars / concentration of nitrogen sources / トリコデルマ抑制 / 対峙培養 / 菌叢上接種 / 培地組成の影響 / 物理的状況 / 二層培養系 / 酸化反応 / イグチ類 / 相互作用 / 種間差 / 菌株間差 / ショウロ / ホンシメジ / 培地条件
Research Abstract

Ectomycorrhizal fungi include valuable edible fungi, such as Tricholoma matsutake, Lyophyllum shimeji, Lactarius hatsudake and fungi of Boletaceae. Fungi of Boletaceae and Rhizopogon rubescens also increase efficiency of forestation. To promote these usage of ectomycorrhizal fungi, it will be an important method to grow ectomycorrhizal fungi in forest soil using their culture. To perform the artificial inoculation of forest soil, damage of ectomycorrhizal fungi by soil inbaiting mycoparasite, such as Trichoderma will be an important problem. To study this problem, the interaction between ectomycorrhizal fungi and Trichoderma was studied by culturing these fungi on agar media. Main results were as follows.
1)Fourteen,stains of 9 species of ectomycorrhizal fungi and two strains of Trichoderma were used.
2)The interaction was performed at rather high temperature and high humidity conditions, in most cases, Trichoderma overgrew ectomycorrhizal fungi. But in several cases, ectomycorrhizal fungi completely Stopped the growth of Trichoderma.
3)In paired culture, Trichoderma spores germinated well, but afterwards the growth of mycelia was retarded by ectomycorhizal fungi.
4)When Trichoderma spores were inoculated on mycelial mat of ectomycorrhizal fungi, the germination of spores were retarded.
5)The kind of media used for the interaction of ectomycorrhizal fungi and Trichoderma, affected the result of interaction. Especially concentrations of carbon source and nitrogen source affected strongly.
6)Water content of media and temperature of interaction also affected the interaction of ectomycorrhizal fungi and Trichoderma.
7)When mycorrhiza formed between pine roots and these ectomycorrhizal fungi were challenged by Trichoderma, in several cases, Hartig net or mantle of mycorrhiza was not observed.
From these results, it is concluded that by selecting the medium condition adequately ectomycorrhizal fungi will stop the growth of Trichoderma in the interaction of these fungi.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Yamada A et al.: "Isolation of Trichoroma matsutake and T.bakamatsutake cultures from field-collected ectomycorrhizas."Mycoscience. 42. 43-50 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada A et al.: "Cultivation of mushrooms of edible ectomycorrhizal fungi associated with Pinus densiflora by in vitro mycorrhizal synthesis II. Morphology of mycorrhizas in open-pot soil."Mycorrhiza. 11. 67-81 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sawahata et al.: "The seasonal change in abundance of Hypogastrura denisana Yosii on agaric mushrooms in relation to its life cycle."Edaphologia. 69. 35-45 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 山田明義, 大政正武: "マツタケ研究の動向"山林. 2003(5). 68-73 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada A et al.: "Isolation of Tricholoma matsutake and T. bakamatsutake cultures from field-collected ectomycorrhizas."Mycoscience. 42. 43-50 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada A et al.: "Cultivation of mushrooms of edible ectomycorrhizal fungi associated with Pinus densiflora by in vitro mycorrhizal synthesis II. Morphology of mycorrhizas in open-pot soil."Mycorrhiza. 11. 67-81 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sawahata et al.: "The seasonal change in abundunce of Hypogastrura denisana Yosii on agaric mushrooms in relation to its life cycle."Edaphologia. 69. 35-45 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada A, Ohmasa M: "Recent advances in the study of Matsutake."Sanrin. 2003(5). 68-73 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大洞健二, 大政正武: "イグチ類きのこの培養及び子実体の細胞当たり核数の研究"日本菌学会第45回大会講演要旨集. 49 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] 澤田大, 大政正武他: "酸化チタン光触媒によるきのこの害菌の殺菌"日本菌学会第45回大会講演要旨集. 45 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山口厳雄, 大政正武他: "褐色腐朽菌の色素脱色反応"日本菌学会第46回大会講演要旨集. 77 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山田明義, 大政正武: "マツタケ研究の動向"山林. 2003.5. 68-73 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 寺岡徹治, 大政正武: "菌根菌による色素脱色に関する研究"第54回日本木材学会大会. (発表予定). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 谷口真, 大政正武: "エノキタケのPseudomonas tolaasiiに対する耐病性検定法の開発"日本菌学会第48回大会. (発表予定). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Sawahata et al.: "The seasonal change in abundance of Hypogastrura denisana Yosii on agaric mushrooms in relation to its life cycle"Edaphologia. 69. 35-45 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 畠山朋之, 山田明義, 大政正武: "イグチ類の菌糸体培養におけるC, Nの利用範囲ならびに相互作用"日本菌学会第46回大会講演要旨集. 46. 76 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山口厳雄, 細野恵理子, 大政正武: "褐色腐朽菌の色素脱色反応"日本菌学会第46回大会講演要旨集. 46. 77 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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