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Studies on investigation and distribution of the neuropeptides in crustaceans

Research Project

Project/Area Number 13660209
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionSuntory Institute for Bioorganic Research

Principal Investigator

YASUDA Akikazu  Yasuda Suntory Institute for Bioorganic Research, Researcher, 主任研究員 (90211614)

Co-Investigator(Kenkyū-buntansha) YASUDA Yoshimi  Yasuda Suntory Institute for Bioorganic Research, Researcher, 研究員 (10270571)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsred swamp crayfish / MALDI-TOF MS / neuropeptide / laser microdissection / orcokinin / SIFamide / tachykinin-related peptide / accessory lobe / Gタンパク質共役型受容体 / 肝膵臓
Research Abstract

Matrix-assisted laser desorption ionization with time-of-flight mass spectrometry (MALDI-TOF MS) has become a rapid and sensitive analytical tool that is having an increasing impact on the direct MS measurement of the secretory granules in the cells. The result is available for the second step that is purification of a given material by monitoring its molecular mass and then sequencing it. The third step is molecular cloning for the precursor of a given substance based on the structural information. The final step is capillary reversed-phase HPLC/Q-Tof analysis with the tissue extract to determine the posttranslational modifications for mature hormone molecules. We have called this total strategy as "topological mass spectrometry analysis". In addition, laser microdissection has become a powerful tool for construction of mass spectrometric morphology in the brain since it is possible to collect single cells from a thin tissue section. In this study, we have identified orcokinin-related peptides, crustacean-SIFamide, and decapod tachykinin-related peptide from the crayfish brain. It is known that the olfactory lobe receives the primary afferent endings of the chemoreceptors on antenna I, but the accessory lobe does not appear to receive a direct input from primary afferent axons. The output pathways from the, olfactory lobe and accessory lobe are provided by the axons of a large population of projection neurons, in which projection neurons innervating the olfactory lobe and accessory lobe innervate the neuropiles of the medulla terminalis and the hemiellipsoid body. The peptides seem to play a significant role in olfaction. Finally, topological mass spectrometry analysis will provide excellent means in micro-characterization of protein/peptide hormone in the field of fish. and insect endocrinology.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Sakai, M., Yamaguchi, T., Watanuki, H., Yasuda, A., Takahashi, A.: "Modulation of fish phagocytic cells by N-terminal peptides of proopiomelanocortin(NPP)"J.Exp.Zool.. 290. 341-346 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, D., Yasuda, A., Naoki, H., Corzo, G., Andriantsiferana, M., Nakajima T.: "IsCT, a novel cytotoxic linear peptide from scorpion Opisthacanthus madagascariensis."Biochem.Biophys.Res.Commun.. 286. 820-825 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda-Kamatani, Y., Yasuda.A.: "Cloning and expression of a cDNA for a putative G protein-coupled receptor from the hematopancreas of the crayfish, Procambarus clarkii."Gen.Comp.Endocrinol.. 125. 25-33 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, A., Amemiya, Y., Yasuda, A., Meguro, H., Kawauchi, H.: "Mass spectrometric detection of proopiomelanocortin(POMC)-related peptides following molecular cloning of POMC cDNA in bigeye tunna Thunnus"Fisheries Science. 68. 1073-1080 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 安田明和: "キャピラリーLC-MSによるタンパク質・ペプチドホルモンの分析"日本比較内分泌学会ニュース. 107. 29-35 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, A., Yasuda, A., Sullivan, C.V., Kawauchi, H.: "Identification of proopiomelanocortin-related peptides in the rostral pars distalis of the pituitary in coelacanth : evolutional implication"Gen.Comp.Endocrinol.. 130. 340-349 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, H., Yasuda, A., Yasuda-Kamatani, Y., Kubo, T., Nakajima, T.: "Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads."Insect Mol.Biol.. 12. 291-298 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawakoshi, A., Hyodo, S., Yasuda, A., Takei, Y.: "A single and novel natriuretic peptide is expressed in the heart and brain of the most primitive vertebrate, the hagfish (Eptatretus burgeri)."J.Mol.Endocrinol.. 31. 209-220 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y., Nozaki, M., Nakajima, T.: "Identification of GYRKPPFNGSIFamide(crustacean-SIFamide) in the crayfish Procambarus clarkii by topological mass spectrometry analysis."Gen.Comp.Endocrinol.. 135. 391-400 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y., Nakajima, T.: "A new strategy for exploring hormones by topological mass spectrometry analysis."Perspective in Comparative Endocrinology. (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y.: "Topological mass spectrometry analysis in crustacean endocrinology."Proceedings of the 21st conference of european comparative endocrinology. (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y., Nakajima, T.: "Perspective in Comparative Endocrinology"A new strategy for exploring hormones by topological mass spectrometry analysis.. 6 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y.: "Proceedings of the 21st conference of european comparative endocrinology"Topological mass spectrometry analysis in crustacean endocrinology.. 4 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai, M., Yamaguchi, T., Watanuki, H., Yasuda, A., Takahashi, A.: "Modulation of fish phagocytic cells by N-terminal peptides of proopiomelanocortin (NPP)"J.Exp.Zool.. 290. 341-346 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, D., Yasuda, A., Naoki, H., Corzo., G., Andriantsiferana., M., Nakajima, T.: "IsCT, a novel cytotoxic linear peptide from scorpion Opisthacanthus madagascariensis."Biochem.Biophys.Res.Commun.. 286. 820-825 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda-Kamatani, Y., Yasuda, A.: "Cloning and expression of a cDNA for a putative G protein-coupled receptor from the hematopancreas of the crayfish, Procambarus clarkii."Gen.Comp.Endocrinol.. 125. 25-33 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, A., Amemiya, Y., Yasuda, A., Meguro, H., Kawauchi, H.: "Mass spectrometric detection of proopiomelanocortin (POMC)-related peptides following molecular cloning of POMC cDNA in bigeye tunna Thunnus obesus."Fisheries Science. 68. 1073-1080 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, A.: "Capillary LC-MS analysis of the protein/peptide hormones (in Japanese)"Newsletter of JSCE. 107. 29-35 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi A., Yasuda, A., Sullivan, C.V., Kawauchi, H.: "Identification of proopiomelanocortin-related peptides in the rostral pars distalis of the pituitary in coelacanth : evolutional implications."Gen.Comp.Endocrinol.. 130. 340-349 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, H., Yasuda, A., Yasuda-Kamatani, Y., Kubo, T., Nakajima, T.: "Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen, and drone heads."Insect Mol.Biol.. 12. 291-298 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawakoshi, A., Hyodo, S., Yasuda, A., Takei, Y.: "A single and novel natriuretic peptide is expressed in the heart and brain of the most primitive vertebrate, the hagfish (Eptatretus burgeri)."J.Mol.Endocrinol.. 31. 209-220 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakajima, T., Naoki, H, Corzo, G., Li, D., Hisada, M., Escoubas, P., Yamaji, N., Nagai, H., Yasuda, A., Andrianstiferana, M., Haupt, J., Oshiro, N.: "A trial of mass spectrometric characterization of femto-molar amount from subtropical islands."J.Toxicol.. 22. 509-520 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y., Nozaki, M., Nakajima, T.: "Identification of GYRKPPFNGSIFamide (crustacean-SIFamide) in the crayfish Procambarus clarkii by topological mass spectrometry analysis."Gen.Comp.Endocrinol.. 135. 391-400 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y., Nakajima, T.: "A new strategy for exploring hormones by topological mass spectrometry analysis. Perspective in Comparative Endocrinology (H.J.Th.Goos, R.K.Rastogi, H.Vaudry, R.Pierantoni, Eds)(Bologna)"Monduzzi Editore. 935-340 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y.: "Topological mass spectrometry analysis in crustacean endocrinology. Proceedings of the 21st conference of european comparative endocrinology(R.Keller, H.Direcksen, D.Sedlmeier, H.Vaudry, Eds)(Bologna)"Monduzzi Editore. 447-450 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, A., Yasuda, A., Sullivan, C.V., Kawauchi, H.: "Identification of proopiomelanocortin-related peptides in the rostral pars distalis of the pituitary in coelacanth : evolutional implication"Gen.Comp.Endocrinol.. 130. 340-349 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takeuchi, H., Yasuda, A., Yasuda-Kamatani, Y., Kubo, T., Nakajima, T: "Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, que"Insect Mol.Biol. 12. 291-298 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawakoshi, A., Hyodo, S., Yasuda, A., Takei, Y.: "A single and novel natriuretic peptide is expressed in the heart and brain of the most primitive vertebrate, the hagfish (Eptatretus burger"J.Mol.Endocrinol.. 31. 209-220 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y., Nozaki, M., Nakajima T.: "Identification of GYRKPPFNGSIFamide (crustacean-SIFamide) in the crayfish Procambarus clarkii by topological mass spectrometry analysis."Gen.Comp.Endocrinol.. 135. 391-400 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahashi, A., Amemiya, Y., Yasuda, A., Meguro, H., Kawauchi, H.: "Mass spectrometric detection of proopiomelanocortin (POMC)-related peptides following molecular cloning of POMO cDNA in bigeye tuna Thunnus obesus"Fisheries Science. 68. 1073-1080 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 安田明和: "キャピラリーLC-MSによるタンパク質・ペプチドホルモンの分析"日本比較内分泌学会ニュース. 107. 29-35 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y.: "Proceedings of the 21st Conference of European Comparative Endocrinologists"MONDUZZI EDITORE. 4 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasuda-Kamatani, Y., Yasuda.A.: "Cloning and expression of a cDNA for putative G protein-coupled from receptor the hepatopancreas of the crayfish. Procambarus clarkii"General and Comparative Endocrinology. 125. 25-33 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasuda, A., Yasuda-Kamatani, Y., Nakajima.T.: "Perspective in Comparative Endocrinology : Unity and Diversity"A new strategy for exploring hormones by topological mass spectrometry analysis. 6 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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