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INTRODUCE OF EFFECTIVE ANTIBIOTICS AND VACCINES ON FORAGE CROPS BY MEANS OF GENETIC ENGINEERING

Research Project

Project/Area Number 13660270
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionUniversity of Miyazaki

Principal Investigator

AKASHI Ryo  UNIVERSlTY OF MIYAZAKI, FACULTY OF AGRICULTURE, ASOCIATE PROFESSOR, 農学部, 助教授 (20253809)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsANTIBIOTICS / FORAGE CROP / GENETIC ENGINEERING / PLANT BIOTECHNOLOGY / TRANSFORMATION / VACCINES / 家畜免疫増強因子 / 形質転換体
Research Abstract

We have improved our previously published technique for plant regenerationfrom seed-derived embryogenic callus of bahiagrass by keeping the callus size small through regular partition. This modifiedculture remains regenerable for at least 18 months and results in a decrease of albino plants among regenerants. Also, in transformation experiments, an increased surface area is available for gene delivery, and a greater number of cells can potentially regenerate. Using a simple self-built particle inflow gun with improved technical features, we have optimized delivery conditions and bombarded the small calli with pDB1, a construct carrying both the GUS reporter gene and a bialaphos resistance gene (bar). Numerous calli have been selected on bialaphos-supplemented medium and subjected to regeneration conditions. These calli remained resistant and continue to display GUS activity. Shoots as well as embryo-like structures formed from resistant calli under regeneration conditions and green pla … More nts were obtained.
Super roots of Lotus corniculatus are a fast growing legume root culture that allows continuous root cloning, direct somatic embryogenesis and mass regeneration of plants under entirely growth regulator-free culture conditions. These features are unique to root cultures and are now stable expressed since the, culture has been isolated almost 5 years ago. In several years of experimentation, all our attempts to directly transform super roots with Agrobacterium failed for unknown reasons. Only recently, by using super root-derived callus as acceptor tissue, we were able to demonstrate transient expression of a reporter gene (GUS) in a tissuederived from super roots. GUS expression was also, visible in roots and shoots regenerated from transformed calli. The super root character is stable maintained through the callus phase. Roots derived from these calli can serve as explants for new super root cultures. This stability of the callus phase makes it unlikely that transformation was made possible through the loss of protective genetic information, such as a spontaneous A. rhizogenes infection, through callus initiation. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Tsuruta S., et al.: "Molecular phylogenetic relationships among fifteen lines of napiergrass (Pennisetum purpureum Schumach) using RAPD analysis"Grassland Science. 48. 37-42 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akashi R., et al.: "Super roots in Lotus corniculatus : A unique tissue culture and regeneration system in a legume species"Plant and Sail. 255. 27-33 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gondo et al.: "Efficient embryogenic callus induction derived from mature seeds and the examination of the genetic transformation condition by particle bombardment in bahiagrass (Paspalum notatumu Flugge)"Grassland Science. 49・1. 33-37 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 明石ら: "バイオテクノロジーを利用した暖地型イネ科牧草の育種"日本草地学会誌. 49・1. 79-87 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 河野ら: "マメ科植物におけるスーパールート"根研究会. 12・1. 3-8 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gondo et al.: "Effect of BAP and cupric sulfate on embryogenic callus formation and plant regeneration in bahiagrass (Paspalum notatumu Flugge)"Grassland Science. 50・1. 55-58 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuruta S., et al.: "Molecular phylogenetic relationships among fiteen lines of napiergrass (Pennisetum purpureum Schumach) using RAPD analysis."Grassland Science. 48. 37-42 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akashi R., et al.: "Super roots in Lotus corniculatus : A unique tissue culture and regeneration system in a legume species."Plant and Soil. 255. 27-33 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gondo et al.: "Efficient embryogenic callus induction derived from mature seeds and the examination of the genetic transformation condition by particle bomberdment in bahiagrass (Paspalum notatumu Flugge)."Grassland Science. 49. 33-37 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akashi et al.: "Fundamental studies on the improvement of some warm-season grasses by means of plant biotechnology."Grassland Science. 49. 79-87 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gondo et al.: "Effect of BAP and cupric sulfate on embryogenic callus formation and plant regeneration in bahiagrass (Paspalum notatumu Flugge)."Grassland Science. 50. 55-58 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 明石: "バイオテクノロジーを利用した暖地型イネ科牧草の育種"日本草地学会誌. 49. 79-87 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 権藤: "バヒアグラス(Paspalum notatum Flugge)における効率的な種子由来エンブリオジェニックカルス誘導法とパーティクルガンによる形質転換条件の検討"日本草地学会誌. 49. 33-37 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kamaruddin et al.: "Variation in morphological and feeding characteristics in plants regenerated from embryogenic suspension cultures derived from a single genotype of bahiagrass (Paspalum notatum Flugge)"Grassland Science. 49. 456-459 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kamaruddin et al.: "Chemical composition and in vitro digestibility of bahiagrass (Paspalum notatum Flugge) regenerated from suspension culture"Bulletin of the Faculty of Agriculture, University of Miyazaki. 50. 25-30 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akashi R., et al.: "Super roots in Lotus corniculatus : A unique tissue culture and regeneration system in a legume species"Plant and Soil. 255. 27-33 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuruta S., et al.: "Molecular phylogenetic relationships among fiteen lines of napiergrass (Pennisetum purpureum Schumach) using RAPD analysis"Grassland Science. 48. 37-42 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akashi R., et al.: "Super roots in Lotus corniculatus : A unique tissue culture and regeneration system in a legume species"Plant and Soil. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Gondo et al.: "Efficient embryogenic callus induction derived from mature seeds and the examination of the genetic transformation condition by particle bomberdment in bahiagrass (Paspalum notatumu Flugge)"Grassland Science. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 明石: "バイオテクノロジーを利用した暖地型イネ科牧草の育種"日本草地学会誌. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 河野: "マメ科植物におけるスーパールート"根研究会. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 河野: "マメ科植物におけるスーパールート"根研究会. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akashi R., et al.: "Bialaphos-resistant cells of dallisgrass(Paspalum dilatatum Poir.) through particle bombardment with a simple self-built inflow gun"Grassland Science. 47. 588-593 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kawamura O., et al.: "Dry mater and lignin distributions in maize(Zea mays L.) internodes"Grassland Science. 47. 578-582 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuruta et al.: "Phylogenetic variation of morphological characteristics, and their relationship with brix and lignin content in napiergrass(Pennisetum purpureum)"Grassland Science. 47. 604-609 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akashi R., et al.: "Super roots in Lotus corniculatus : A unique tissue culture and regeneration system in legume species"Plant and Soil. (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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