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Studies on in vitro fertilization and embryo production by intracytoplasimc sperm injection (ICSI) in cattle

Research Project

Project/Area Number 13660276
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

FUKUI Yutaka  Obihiro Univ., Animal Science, Professor, 畜産学部, 教授 (40120547)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsMicro-manipulated insemination / In vitro fertilization / Embryo Development / Sperm injection / Immature oocyte / Bovine / 顕微受精
Research Abstract

The research was aimed to establish an intracytoplasmic sperm injection (ICSI) in cattle by studying various conditions for spermatozoa and oocytes. For spermatozoa, (1) sperm pre-treatment, (2) sperm immobilization (3) concentrations of polyvinylpyrolidone (PVP), and for oocytes, (1) oocyte maturation period, (2) centrifugation to polarize the ooplasmic lipid, (3) oocyte activation methods, were investigated to prove a hypothesis that oocyte activation after ICSI is not necessary in cattle, and the blastocysts were transferred to recipient heifers to obtain normal calves. The results obtained were; (1) Bull difference varied the outcomes by conventional in vitro fertilization (IVF) and by ICSI. Namely, male pronuclear formation rate was improved by ICSI using bull semen with low IVF rates. (2) There was no significant difference in the male pronuclear formation rate among the sperm types (motile, immotile or dead sperm). Ca ionophore and sperm immobilization enhanced the fertilizability. (3) Oocyte survival and normal fertilization rate were improved by centrifugation, 4% PVP concentration and sperm-tail cutting. The subsequent cleavage and blastocyst development were 72% and 23%, respectively. (4) After transfer 8 blastocysts derived by ICSI without oocyte activation, 4 out of 7 heifers were pregnant and gave 5 normal calves. These results indicate that a bovine ICSI method was been established, and showed that oocyte activation after ICSI is not necessary in cattle.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] H.Wei: "Effects of ball, sprm type and sperm pretreatment on male prondclear formation by ZCSZ in catlle"Reproduction, Fertility and Development. 11. 59-65 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Wei: "Technical improvement for intractoplasmic sperm infection (ZCSZ) in catlle"Journal of Reprodaction and Development. 46. 403-407 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Wei: "Fatilizability of orine, borine or minke whale sleecm intrary to plasmically infected into bovine oocytes"Zysote. 8. 267-274 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Wei: "Births of calves devived from embryos produced by intracytoplasmic sperm injection with out exosompus"Zysote. 10. 149-153 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Wei: "Births of calves derived from intracytoplasmic sperm infection without exosenous oocyte activation"Theriosenology. 57(Abstract). 758 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Wei: "Effects of bull, sperm type and sperm pre-treatment on male pronuclear formation by ICSI in cattle"Preproduction, Fertility and Development. 11-1. 59-65 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Wei: "Technical improvement for intra-cytoplasmic sperm injection (ICSI) in cattle"Journal of Reproduction and Development. 46-6. 403-407 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Wei: "Fertilizability of ovine, bovine or minke whale sperm intracytoplasmically injected into bovine oocytes"Zygote. 8. 267-274 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Wei: "Births of calves from embryos produced by intracytoplasmic sperm injection without exogenoces oocyte activation"Zygote. 10. 149-153 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Wei: "Births of calves from intracytoplasmic sperm injection without exogenous oocyte activation"Theriogenology. 57-1 (Abstract). 758 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Wei: "Effects of bull, sperm type and sperm pretreatment on made pronuclear formation by ICSI in cattle"Reproduction, Fertility and Development. 11・1. 59-65 (1999)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Wei: "Technical improvement for intracytoplasmic sperm injection (ICSI) in cattle"Journal of Reproduction and Development. 46・6. 403-407 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Wei: "Fertilizability of ovine, bovine or minke whale sperm intracytoplasmically injected into bovine oocytes"Zygote. 8. 267-274 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Wei: "Births of calves derived from embryos produced by intracytoplamic sperm injection without exogenous oocyle activation"Zygote. 10. 149-153 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Wei: "Births of calves derived from intracytoplasmic sperm injection without exogenous oocyte activation"Theriogenilogy. 57・1. 758 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Wei: "Births of calves derived from intracytoplamic sperm injection without exgenous oocyte activation"Theriogenology. 57・1(Abctreect). 758 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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