Regulatory Mechanism of Glucocorticoid Action in the Bovine Ovary
| Project/Area Number |
13660277
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
TETSUKA Masafumi Obihiro University of Agriculture and Veterinary Medicine, Department of Animal Science, Lecturer, 畜産学部, 講師 (40311526)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Akio Obihiro University of Agriculture and Veterinary Medicine, Department of Animal Science, Associate Professor, 畜産学部, 助教授 (10192767)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | OVARY / GLUCOCORTICOID / 11β-HSD / FOLLICLE / CL / CORTISOL / 糖質コルチコイドレセプター |
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| Research Abstract |
Glucocorticoid (G) is produced by adrenal cortex and acts as an important modulator in several bodily organs. Ovary is a G target organ and direct actions of G on ovarian function have been reported in several species including cattle. Thus the ovarian levels of G must be maintained within an optimum range for appropriate ovarian functions. In this study, we investigated the mechanism that regulates ovarian G level and action, i.e. the expression of G metabolizing enzyme (11β-HSD) and G receptor (GR) in bovine follicles and corpora lutea (CL). Follicular and CL samples were obtained from Holstein cows in local slaughterhouse. The expression of 11β-HSD and GR Mrna was semi-quantified by RT-PCR and concentrations of follicular steroids were determined by EIA. The results obtained in this study indicate that 1) the bovine ovarian tissues express two isoforms of 11β-HSD and GR, 2) the expression of these G regulating factors is affected by the physiological status of follicles and CL, 3) follicular 11β-HSD may be a key regulator that modulates follicular G environment, and 4) an excess G might suppress follicular estrogen synthesis which is an important event leading to follicular maturation and ovulation. These results suggest that the ovary is not a passive G target organ at a mercy of circulating G but an active organ that can create its own local G environment suites for follicular and luteal functions.
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Report
(3 results)
Research Products
(3 results)
