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Therapy for Viral Disease by Recombinant Virus or by Recombinant DNA

Research Project

Project/Area Number 13660299
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionYamaguchi University

Principal Investigator

KAI Kazushige  Yamaguchi University, Fac. Agriculture, Professor, 農学部, 教授 (60085628)

Co-Investigator(Kenkyū-buntansha) HONNDA Eiichi  Yamaguchi University, Fac. Agriculture, Associate Professor, 農学部, 助教授 (30271745)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsMultivalent vaccine / Simultaneous expression of genes / MuLV vector / マウスレトロウイルスベクター
Research Abstract

The virus vector system that transduces some genes simultaneously seems to be very useful for various gene transducing therapy. In this research, we intended to develop a retrovirus vector that carries two genes in the gag and the env gene regions of Friend murine leukemia virus (F-MuLV). We madevariousvectors that lost gag initiation codons, bare various types of Human Cytomegalovirus (CMV) promoter in stead of the enhancer sequence of LTR of MULV, and/or bare EGFP gene in the gag gene region and H- or F-gene of canine distemper virus (CDV) in the env gene region of MuLV. Results are followings; 1)The lost of initiation codons did not affect the expression of introduced gene in gag gene region, 2)The introduction of CMV promotor was necessary for the expression of introduced genes in the xeno-genic cells than mouse or rat cells although all of introduced segments from CMV promotor showed no difference among them (manuscript in preparation), 3)Both gag gene and env gene regions were responsible for introducing exogenous genes, 4)The H gene of CDV induced cytopathic effect and the F gene of CDV iduced cell-fusion on transfected Vero cells, 5)The co-transfection of both H- or F-bearing vector DNA showed slow progress of cell fusion and the process of cell fusion was possibly monitered by the introduced EGFP gene product (manuscript in preparation).

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report

Research Products

(32 results)

All Other

All Publications (32 results)

  • [Publications] 相馬武久: "家庭猫における猫免疫不全ウイルス抗体、猫白血病ウイルス抗原及び猫コロナウイルス抗体の陽性率"日本獣医師会雑誌. 55. 89-93 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Mochizuki: "Complement-mediated neutralization of canine distemper virus in vitro : Cross-reaction between vaccine Onderstepoort and field KDK-1 strains with different"Clinical AND Diagnostic Laboratory Immunology. 9. 921-924 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kakuni M: "Histology of vermiform appendix-like organ in slow loris"Exp Anim. 52. 71-75 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanabe S: "Expression of mRNA of chemokine receptor CXCR4 in feline mammary adenocarcinoma"Vet Rec. 151. 729-733 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kakuni M: "Histological study on intestinal diverticulum of tree shrew (Tupaia javanica)"Exp Anim. 1;51. 411-415 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimokawa T: "A morphological study of the thyroid gland in Risso's Dolphin, Grampus griseus"J Vet Med Sci. 64. 509-512 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Namba Y: "A study of reproductive performance in pregnant, IL-2 receptor beta-chain overexpressed transgenic mice"J. Vet. Med. Sci. 63. 99-101 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kitamura N: "Immunohistochemical study of the ontogeny of prochymosin-and pepsinogen-producing cells in the abomasum of sheep"Anat. Histol. Embryol. 30. 231-235 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hondo E: "Molecular Cloning and Expression of Suppressor of Potassium Transport Defect 3 (SKD3) in Rat Testis"J. Reprod. Dev.. 47. 173-180 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morigaki T: "Cycle of the seminiferous epithelium in the Java Fruit Bat (Pteropus vampyrus) and the Japanese Lesser Horseshoe Bat (Rhinolophus cornutus)"J. Vet. Med. Sci. 63. 773-779 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Mochizuki, M. Motoyoshi, K. Maeda, K. Kai: "Complement-mediated neutralization of canine distemper virus in vitro: Cross-reaction between vaccine Onderstepoort and field KDK-1 strains with different hemagglutinin gene characterictics"Clinical AND Diagnostic Laboratory Immunology. 9. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morigaki T., Kurohmaru M., Kanai Y., Mukohyama M., Hondo E., Yamada J., Agungpriyono S., Hayashi Y.: "Cycle of the seminiferous epithelium in the Java Fruit Bat (Pteropus vampyrus) and the Japanese Lesser Horseshoe Bat (Rhinolophus cornutus)"J. Vet. Med. Sci.. 63. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hondo E., Kobayashi T., Aita T., Kitamura N., Yamada J., Namba Y., Nagahama Y., Kiso Y.: "Molecular Cloning and Expression of Suppressor of Potassium Transport Defect 3 (SKD3) in Rat Testis"J. Reprod. Dev.. 47. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kitamura N., Tanimoto A., Hondo E., Andren A., Cottrell D F., Sasaki M., Yamada J.: "Immunohistochemical study of the ontogeny of prochymosin- and pepsinogen-producing cells in the abomasum of sheep"Anat. Histol. Embryol. 30. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Namba Y., Hondo E., Morimoto M., Nakamura O., Kusakabe K., Ito M., Saito S., Sagara E., Kiso Y.: "A study of reproductive performance in pregnant, IL-2 receptor beta-chain overexpressed transgenic mice."Vet Rec. 14. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanabe S., Nakadai T., Furuoka H., Oomachi T., Kobayashi Y., Omata Y., Koyama T., Hondo E., Uzuka Y., Sarashina T., Ducusin RJ., Shida T.: "Expression of mRNA of chemokine receptor CXCR4 in feline mammar adenocarcinoma"Vet. Rec.. 151. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kakuni M., Makita T., Wijayanto H., Hondo E., Kiso Y.: "Histological study on intestinal diverticulum of tree shrew (Tupaia javanica)"Exp. Anim.. 151. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimokawa T., Nakanishi I., Hondo E., Iwasaki T., Kiso Y., Makita T.: "A morphological study of the thyroid gland in Risso's Dolphin, Grampus griseus"J. Vet. Med. Sci.. 64. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 相馬武久: "家庭猫における猫免疫不全ウイルス抗体、猫白血病ウイルス抗原及び猫コロナウイルス抗体の陽性率"日本獣医師会雑誌. 55. 89-93 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Mochizuki: "Complement-mediated neutralization of canine distemper virus in vitro : Cross-reaction between vaccine Onderstepoort and field KDK-1 strains with different"Clinical AND Diagnostic Laboratory Immunology. 9. 921-924 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kakuni M: "Histology of vermiform appendix-like organ in slow loris"Exp Anim. 52. 71-75 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tanabe S: "Expression of mRNA of chemokine receptor CXCR4 in feline mammary adenocarcinoma"Vet Rec. 1;51. 729-733 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kakuni M: "Histological study on intestinal diverticulum of tree shrew (Tupaia iavanica)"Exp Anim. 151. 411-415 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimokawa T: "A morphological study of the thyroid gland in Risso's Dolphin, Grampus griseus"JVet Med Sci. 64. 509-512 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Namba Y: "A study of reproductive performance in pregnant, IL-2 receptor beta-chain overexpressed transgenic mice"J. Vet. Med. Sci. 63. 99-101 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kitamura N: "Immunohistochemical study of the ontogeny of prochymosin-and pepsinogen-producing cells in the abomasum of sheep"Anat. Histol. Embryol. 30. 231-235 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hondo E: "Molecular Cloning and Expression of Suppressor of Potassium Transport Defect 3(SKD3)in Rat Testis"J. Reprod. Dev.. 47. 173-180 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morigaki T: "Cycle of the seminiferous epithelium in the Java Fruit Bat (Pteropus vampyrus) and the Japanese Lesser Horseshoe Bat (Rhinolophus cornutus)"J. Vet. Med. Sci. 63. 773-779 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morigaki T.: "Cycle of the seminiferous epithelium in the Java Fruit Bat (Pteropus vampyrus) and the Japanese Lesser Horseshoe Bat (Rhinolophus cornutus)."J. Vet. Med. Sci.. 63巻. 773-779 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hondo E.: "Molecular Cloning and Expression of Suppressor of potassium Transport Defect 3(KSD3) in Rat Testis."J. Reprod. Dev.. 47巻. 173-180 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kitamura N.: "Immunohistochemical study of the ontogeny of prochymosin-and pepsinogen-producing cells in the abomasum of sheep."Anat. Histol. Embryol.. 30巻. 231-235 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Namba Y.: "A study of reproductive performance in pregnant, IL-2 receptor beta-chain overexpressed transgenic mice."J. Vet. Med. Sci.. 63巻. 99-101 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-03-31   Modified: 2016-04-21  

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