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Studies on Protein Structure and Physiological Function of Ovalbumin with Property of Molecular Chaperon

Research Project

Project/Area Number 13660301
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKagoshima University

Principal Investigator

SUGIMOTO Yasushi  Kagoshima University, United Graduate School of Agricultural Sciences, Professor, 大学院連合・農学研究科, 教授 (10100736)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Mitsuharu  Kagoshima University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (30157383)
AOKI Takayoshi  Kagoshima University, Faculty of Agriculture, Professor, 農学部, 教授 (70034460)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsovalbumin / S-ovalabumin / serpin / organogenesis / molecular chaperon / chick embryo / knockout / 鶏発生 / DSC / ニワトリ / プロテアーゼインヒビター / 熱安定性 / 構造変化 / タンパク構造 / 胚発生
Research Abstract

Ovalbumin, which is the major albumen protein having carbohydrate and phosphate residues, belongs to the serine protease inhibitor superfamily. However, ovalbumin is not inhibitory against proteases, and, therefore, its function remains uncertain except for a possible contribution as a nutrient source. We reported previously that ovalbumin molecules were transported from albumen to the embryo via egg yolk, amniotic fluid, serum etc, without degradation, and distributed in various organs including the central nervous system, while these molecules undergo changes in secondary conformation and attain a heat-stable state (this state of molecules will be called 'HS-ovalbumin'). Here we showed that HS-ovalbumin is distinguishable from the known heat-stable molecular form named S-ovalbumin by a presently prepared monoclonal antibody and that HS-ovalbumin arises from its native form (N-ovalbumin) in developing eggs. We also established in vitro culture conditions, under which embryos isolated from eggs at and after stage 4 of embryogenesis could be successfully cultured for at least 24 h. When the remnant fractions of endogenous ovalbumin was neutralized by using a polyclonal antibody, the isolated embryos could not grow normally, being particularly irregular in the brain tissue, which hardly undergo the neural tube closure occurring in normal embryos at stage 9. The embryos were rescued from the aberration by adding an excess amount of ovalbumin to the medium at an early period of culture. HS-ovalbumin showed a higher rescue rate than the N-and S-counterparts. Our findings demonstrated that ovalbumin is indispensable to, and probably plays a crucial role in, the embryonic organogenesis.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Sugimoto, Y., Sanuki, S., Ibrahim, H.R., Aoki, T., Kusakabe, T., Koga, K.: "Occurence of ovalbumin in ovarian yolk of chicken during oogenesis."Biochim.Biophys.Acta. 1526. 1-4 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kusakabe T, Sugimoto Y, Maeda T, Nakajima Y, Miyano M, Nishikawa J, Tone S, Kawaguchi Y, Koga K, Ohyama T.: "Linearization and integration of DNA into cells preferentially occurs at intrinsicallycurved regions from human LINE-1 repetitive element."Gene. 274. 71-81 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiyoshi, M., Takamune, K., Mite, K., Kubo, H., Sugimoto, Y., Katagiri, C.: "Oviductin, the oviductal protease that mediates gamete interaction by affecting the vitelline coat in Bufo japonicas : its molecular cloning and analyses of expression and posttranslational activation."Developmental Biology. 243. 176-184 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ota A, Kusakabe T, Sugimoto Y, Takahashi M, Nakajima Y, Kawaguchi Y, Koga K.: "Cloning and characterization of testis-specific tektin in Bombyx mori."Comp.Biochem.Physiol.B Bfochem.Mol.Biol.. 133. 371-382 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakano T, Salvador AS, Tamochi J, Sugimoto Y, Ibrahim HR, Toba Y, Aoe S, Kawakami H, Aoki T.: "Phosphorylation of starch and dextrin by dry-heating in the presence of phosphate and their calcium phosphate-solubilizing ability."Nahrung. 474. 274-278 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li CP, Salvador AS, Ibrahim HR, Sugimoto Y, Aoki T.: "Phosphorylation of egg white proteins by dry-heating in the presence of phosphate."J.Agric.Food Chem.. 51. 6808-6815 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li CP, Salvador AS, Ibrahim HR, Sugimoto Y, Aoki T.: "Phospholylation of egg white proteins by dry-heating in the presence of phosphate."J.Agric.Food Chem.. 51. 6808-6815 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakano T, Salvador AS, Tamochi J, Sugimoto Y, Ibrahim HR, Toba Y, Aoe S, Kawakami H, Aoki T.: "Phosphorylation of starch and dextrin by dry-heating in the presence of phosphate, and their calcium phosphate-solubilizing ability."Nahrung. 474. 274-278 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ota A, Kusakabe T, Sugimoto Y, Takahashi M, Nakajima Y, Kawaguchi Y, Koga K.: "Cloning and characterization of testis-specific tektin in Bombyx mori."Comp.Biochem.Physiol.B Biochem.Mol.Biol.. 133. 371-382 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiyoshi, M., Takamune, K., Mita, K., Kubo, H., Sugimoto, Y., Katagiri, C.: "Oviductin, the oviductal protease that mediates gamete interaction by affecting the vitelline coat in Bufo japonicus : its molecular cloning and analyses of expression and posttranslational activation."Dev.Biol.. 243. 176-184 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kusakabe T, Sugimoto Y, Maeda T, Nakajima Y, Miyano M, Nishikawa J, Tone S, Kawaguchi Y, Koga K, Ohyama T.: "Linearization and integration of DNA into cells preferentially occurs at intrinsicallycurved regions from human LINE-1 repetitive element."Gene. 274. 71-81 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugimoto, Y., Sanuki, S., Ibrahim, H.R., Aoki, T., Kusakabe, T., Koga, K.: "Occurence of ovalbumin in ovarian yolkof chicken during oogenesis."Biochim.Biophys.Acta. 1526. 1-4 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li CP, Salvador AS, Ibrahim HR, Sugimoto Y, Aoki T: "Phosphorylation of egg white proteins by dry-heating in the presence of phosphate"Journal of Agricultural Food Chemistry. 51・23. 6808-6815 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakano T, Salvador AS, Tamochi J, Sugimoto Y, Ibrahim HR, Toba Y, Aoe S, Kawakami H, Aoki T: "Phosphorylation of starch and dextrin by dry-heating in the presence of phosphate, and their calcium phosphate-solubilizing ability"Nahrung. 47・4. 274-278 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiyoshi, M., Takamune, K., Mita, K., Kubo, H., Sugimoto, Y., Katagiri C.: "Oviductin, the oviductal protease that mediates gamete interaction by affecting the vitelline coat in Bufo japonicus : Its molecular cloning and analyses of expression and post-translational activation"Developmental Biology. 243. 176-184 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ota, A., Kusakabe, T., Sugimoto, Y., Takahashi, M., Koga, K.: "Cloning and characterization of testis-specific tektin in Bombyx mori"Comparative Biochemistry and Physiology. 133. 371-382 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 杉元康志, 篠原広志, 宮崎有希子: "胚発生に働く卵白タンパク質オボアルブミン"化学と生物. 40. 216-219 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kusakabe T, Sugimoto Y, et al.: "Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element"Gene. 274. 271-281 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sugimoto Y. et al.: "Occurrence of ovalbumin in ovarian yolk of chicken during oogenesis"Biochim Biorhys Acta. 1526. 1-4 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Aoki, T., Hiidome, Y., Sugimoto, Y.et al.: "Modification of ovalbumin with oligogalacturonic acids through the Maillard reaction"Food Res. Int.. 34. 127-132 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiyoshi, M., Sugimoto, Y.et al.: "Oviductin, the oviductal protease that mediates gamete interaction by affecting the vitelline coat in Bufo japonicus : Its molecular cloning and analyses of expression and post-translational activation"Developmental Biology. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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