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Elucidation of vasoreactive diversity induced by bradykinin -studies of G-proteins function and bradykinin metabolism -

Research Project

Project/Area Number 13660302
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKagoshima University

Principal Investigator

MIYAMOTO Atsushi  Kagoshima University, Faculty of Agriculture, Associate professor, 農学部, 助教授 (70219806)

Co-Investigator(Kenkyū-buntansha) NISHIO Akira  Kagoshima University, Faculty of Agriculture, Professor, 農学部, 教授 (90133181)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsBradykinin / Basilar artery / Vasoreactivity / Tyrosine phosphorylation / cultured endothelial cell / NO production / Losartan / HOE140 / G-蛋白質 / チロシンキナーゼ阻害薬 / チロシンフォスファターゼ阻害薬 / 冠状動脈 / ブタ / ウシ
Research Abstract

(1)Nitric oxide (NO) is spontaneously released from cultured porcine basilar arterial endothelial cells under no stimulation conditions. Bradykinin (BK) enhanced the NO production in a concentration-dependent manner. The enhanced NO production was inhibited by treatments of a selective B_2 receptor antagonist, HOE140, or a NO synthase inhibitor, L-nitro-arginine.
(2)Angiotensin (Ang) II induced a contraction in isolated porcine basilar arterial ring. A selective B_2 antagonist, HOE140, inhibited the AngII-induced contraction, while an Ang converting enzyme (ACE) inhibitor, captopril, enhanced the Ang II-induced contraction.
(3)BK induces endothelium-dependent relaxation following by contraction in isolated porcine basilar arterial ring. In the presence of L-nitro-arginine (10^<-4> M), BK induced contraction but not relaxation. The BK-induced contraction was not inhibited by a selective AT_1 receptor antagonist, losartan.
These results suggest that there might be some interaction between bradykinin and Ang receptors. Further experiments appears to be needed in this point.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Miyamoto, A., Murata, S., Nishio, A.: "Role of ACE and NEP in bradykinin-induced relaxation and contraction response of isolated porcine basilar artery."Naunyn-Schmiedeberg's Archives of Pharmacology. 365. 365-370 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Atsushi Miyamoto, Shin Murata, Akira Nishio: "Role of ACE and NEP in bradykinin-induced relaxation and contraction response of isolated porcine basilar artery."Naunyn-Schmiedeberg's Arch.Pharmacol. 365. 365-370 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyamoto, A.: "Role of ACE and NEP in bradykinin-induced relaxation and contraction response of isolated porcine basilar artery"Naunyn-Schmiedeberg's Archives of Pharmacology. 365・5. 365-370 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyamoto, A.: "Role of ACE and NEP in bradykinin-induced relaxation and contraction response of isolated porcine basilar artery"Naunyn-Schmiedeberg's Archives of Pharmacology. 365・5. 365-370 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miyamoto, A.: "Role of ACE and NEP in bradykinin-induced relaxation and contraction response of isolated porcine basilar artery"Naunyn-Schmiedeberg's Archives of Pharmacology. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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