| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
1. Expression of bovine paracellin-1 mRNA
The expression of bovine paracellin-1 mRNA was tested in 2 normal Japanese Black cattle and one with renal tubular dysplasia (RTD) associated with a deletion of exons 1 to 4 in the paracellin-1 gene. The kidney, brain, heart, lung, liver, spleen, pancreas, skin, skeletal muscle, hoof, rumen, reticulum, psalterium, abomasum, jejunum and colon were collected and total RNA was isolated from these tissues. The expression of paracellin-1 mRNA was determined with the use of RT-PCR and nested PCR. The products were fractionated with agarose gel electrophoresis. Paracellin-1 mRNA was detected only in kidney of the normal cattle, and not in that of the RTD bovine, suggesting that the paracellin-1 protein may be related to renal tubular dysplasia.
2. Renal reabsorption of magnesium and calcium by cattle with renal tubular dysplasia
The concentrations of magnesium and calcium in the serum and urine and their rates of clearance were determined in 27 normal cattle and cattle that were 17 heterozygous or 33 homozygous for renal tubular dysplasia (RTD). In the RTD cattle the serum magnesium concentrations ranged from normal to slightly higher levels, while serum calcium concentrations tended to be lower. The concentrations of magnesium and calcium in the urine were not significantly different among the three groups. The creatinine clearance and the clearance rate of magnesium and calcium were significantly lower in the RTD cattle than in the normal cattle. The ratio of the reabsorption rate to clearance of magnesium was significantly lower in the cattle with RTD than in the normal cattle, but this ratio for calcium was not different. In the RTD cattle the rate of reabsorption of magnesium by the kidneys was low, suggesting that renal tubular magnesium reabsorption was impaired. This defective reabsorption of magnesium may be associated with the deletion of the paracellin-1 gene.
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