| Project/Area Number |
13660320
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied veterinary science
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
HAMANA Katsumi Kagoshima University, College of Agriculture, Professor, 農学部, 教授 (30011977)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAMIMURA Shunichi Kagoshima University, College of Agriculture, Associate Professor, 農学部, 助教授 (90233949)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | Oestrone Sulphate / EIA / Pregnancy monitoring / Plasma oestrone sulphate / Milk oestrone sulphate / Urine oestrone sulphate / Fecal oestrone sulphate / エストロンサルフェート濃度 / 乳汁中OS濃度 / 血液中OS濃度 / 胎子の成熟度 |
|---|
| Research Abstract |
Plasma concentrations of oestrone sulphate (OS), which is a major placental hormone were measured using an enzyme immunoassay (EIA) to monitor pregnancy status in pregnant animals. Concurrently, urine OS, milk OS and fecal OS were measured. Samples during pregnancy were collected monthly from cows and mares. EIA protocol was antigen coated assay using high binding capacity immunoplate and mouse monoclonal antibody for OS. Urine creatine was used for adjusting urine dilution.
OS concentrations in plasma samples increased more than 1.1 ng/ml after d 140 in pregnant cows, which was significantly higher than those in non-pregnant cows (0.36 ng/ml). OS concentrations in milk showed 236 pg/ml after d 100 in pregnant cows, which was also significantly higher than those in non-pregnant cows (14 pg/ml). Os concentrations in fecal samples showed significant correlation with those in plasma samples. OS concentrations in urine samples ranged from 0.16 μg/mg cre in non-pregnant mares to 0.46 μg/mg cre in second month pregnancy. OS concentrations in plasma samples ranged from 19.7 ng/ml in non-pregnant to 31.5 ng/ml in first month of pregnant mares.
OS concentrations in milk, urine and feces determined by EIA were non-invasive sampling, and showed significantly higher levels after second month pregnancy compared to those in non-pregnant cows and mares.
|
