| Project/Area Number |
13660332
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | DEPARTMENT OF IMMUNOLOGY, NATIONAL INSTITUTE OF INFECTIOUS DISEASES |
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Principal Investigator |
SAKAGUCHI Masafflro NATIONAL INSTITUTE OF INFECTIOUS DISEASES, DEPARTMENT OF IMMUNOLOGY, SENIOR RESEARCHER, 免疫部, 主任研究官 (20170590)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIMOTO Majime THE UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, PROFESSOR, 大学院・農学生命科学研究科, 教授 (60163804)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Dog / IgE / Vaccine / Anaphylaxis / アナフィラキシィー / アレルギー性 |
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| Research Abstract |
It has been reported allergic reactions for vaccines such as anaphylaxis as adverse reactions after vaccination in the dog, and it becomes a large problem in the field of veterinary medicine. The purpose of this study is to analyze the allergen induced allergic reactions after this vaccination. We measured the level of bovine serum albumin (BSA) in 12 available mixed vaccines for dogs by the sandwich ELSIA method and found that high levels (60 μg- 5mg/dose) of BSA were included in these vaccines. It is considered that BSA might be derived from fetal calf serum (FCS) in culture medium for virus and from BSA in culture medium for Leptospira. Further, we measured specific IgE for vaccines in the 10 dogs that caused allergic reactions such as tanaphylaxis after the vaccination by the fluorescent ELISA method. Of 10 dogs, eight had specific IgE to vaccines. In addition, we measured specific IgE for FCS and gelatin as a stabilizer. All eight dogs had specific IgE for FCS and/or gelatin. From these results, it is suggested that allergens induced anaphylaxis after the vaccination in the dog might be components in FCS and gelatin.
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