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Immunohistochemical Study on the Localization of Amphiphysin Ir, Novel Retina-Specific Variants of Amphiphysin I, in the Rat Retina

Research Project

Project/Area Number 13670014
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionOkayama University

Principal Investigator

HOSOYA Osamu  Okayama University Neuroanatomy & Neurobiology Research associate, 大学院・医歯学総合研究科, 助手 (90304310)

Co-Investigator(Kenkyū-buntansha) TSUTSUI Kimiko  Okayama University Neuroanatomy & Neurobiology Professor, 大学院・医歯学総合研究科, 教授 (70144748)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsamphiphysin I / retina / splice variant / ribbon synapse / immunohistochemistry / immunoprecipitation / endocytosis / actin cytoskeleton / スプライスバリアント
Research Abstract

Amphiphysin I (amph I) is an SH3 domain-containing protein that has been implicated in synaptic vesicle recycling through clathrin-mediated endocytosis. We have recently cloned novel splice variants of amph I, amph Ir, that are specifically expressed in retina. To provide insight into the function of amph Ir, we examined its precise localization by immunocytochemistry at light and electron microscopic levels. We found that amph Ir is specifically localized at ribbon synapses of photoreceptor and bipolar cells. In addition, immunoprecipitation experiments revealed that amph Ir is bound to the amph I isoform, amph II, an endocytic protein, clathrin, and a cytoskeletal protein, actin. The colocalization of amph Ir and these proteins in retinal sections was confirmed by double-labeling immunofluorescence. These findings suggest that amph Ir plays distinctive roles in retinal ribbon synapses via the interaction with amph II, clathrin, and actin.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] K.Tsutsui: "Immunohistochemical analyses of DNA topoisomerase II isoforms in developing rat cerebellum"J.Comp.Neurol.. 431. 228-239 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Watanabe: "Expression of amphiphysin I in Sertoli cells and its implication in spermatogenesis"Biochem.Biophys.Res.Commun.. 287. 739-745 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] O.Hosoya: "Distribution of amphiphysin Ir, a novel retina-specific variant of amphiphysin I, in the rat retina"Neurosci.Res.. Suppl.25. S73 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Iguchi: "Localization of dynamin 2 in rat semniferous tubes during the spermatogenic cycle"Acta.Med. Okayama. 56. 205-209 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Terada: "Novel splice variants of amphiphysin I are expressed in retina"FEBS Letters. 519. 185-190 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tsutsui: "Immunohistochemical analyses of DNA topoisomerase II isoforms in developing rat cerebellum"J.Comp.Neurol. 431. 228-239 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Watanabe: "Expression of amphiphysin I in Sertoli cells and its implication in spermatogenesis"Biochem.Biophys.Res.Commun.. 287. 739-745 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] O.Hosoya: "Distribution of amphiphysin Ir, a novel retina-specific variant of amphiphysin I, in the rat retina"Nerosci.Res.Suppl.. 25. S73 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Iguchi: "Localization of dynamin 2 in rat seminiferous tubules during the spermatogenic cycle"Acta Med. Okayama. 56. 205-209 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Terada: "Novel splice variants of amphiphysin I are expressed in retina"FEBS Lett.. 519. 185-190 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tsutsui: "Immunohistochemical analyses of DNA topoisomerase II isoforms in developing rat cerebellum"J. Comp. Neurol.. 431. 228-239 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Watanabe: "Expression of amphiphysin I in Sertoli cells and its implication in spermatogenesis"Biochem. Bioph. Res. Co.. 287. 739-745 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] O.Hosoya: "Distribution of amphiphysin Ir, a novel retina-specific variant of amphiphysin I, In the rat retina"Neurosci. Res.. Suppl.25. S73 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Iguchi: "I.ocalization of dynamin 2 in rat semniferous tubes during the spermatogenic cycle"Acta. Med. Okayama. 56. 205-209 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Terada: "Novel splice variants of amphiphysin I are expressed in retine"FEBS Letters. 519. 185-190 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Tsutsui: "Immunohistochemical analyses of DNA topoisormerase II isoforms in developing rat cerebelium"J.Comp.Neurol.. 431. 228-239 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Watanabe: "Expression of amphiphysin I in sertoli cells and its implication in spermatogenesis"Biochom.Bioph.Res.Co.. 287. 739-745 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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