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Diversity of tight junction protein, claudin, in mouse tissues and significance of binding between claudin and ZO-1

Research Project

Project/Area Number 13670018
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyushu University

Principal Investigator

NISHII Kiyomasa (2002)  Kyushu University, Graduate School of Medical Sciences, Assistant Prof., 大学院・医学研究院, 助手 (20264020)

稲井 哲一朗 (2001)  九州大学, 大学院・医学研究院, 講師 (00264044)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Keiichiro  Kyushu University, Graduate School of Medical Sciences, Associate Prof., 大学院・医学研究院, 助教授 (20172398)
SHIBATA Yosaburo  Kyushu University, Graduate School of Medical Sciences, Prof., 大学院・医学研究院, 教授 (90037482)
西井 清雅  九州大学, 大学院・医学研究院, 助手 (20264020)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordstight junction / claudin / ZO-1 / blood-brain barrier / RT-PCR / freeze-fracture / MDCK cell / clauclin / freeze fracture / MDCK cells
Research Abstract

Claudin (CL) is an integral membrane protein of tight junction (TJ) and claudin protein family consists of more than 20 isoforms. Morphology and function of TJ vary in epithelia and endothelia in different tissues. It is thought that compositional differences of CLs may affect morphology and function of TJs. Blood brain barrier of embryonic brain is immature but the barrier becomes mature with growth. Thus, we analyzed mRNA expression of CLs by RT-PCR. CL-1, -5, -10, -12, and -15 were expressed in 8-week-old mouse aorta, suggesting that vascular endothelial cells expressed CL-1, -5, -10, -12, and -15. In contrast, CL-1, -5, -10, and -12 were expressed in adult brain but CL-15 was not. Compared with adult brain, much less CL-10 was expressed in embryonic 18-day-old brain. Taken together, brood brain barrier may be different from others because brain endothelial cells expressed CL-10 with growth and did not express CL-15. Next, we introduced claudin-10 or -15 into MDCK cells to examine which type of TJ strand was formed by them. CL-10 formed TJ strand with E-associated particles but CL-15 formed that with P-associated ones. In general, it is thought that E-associated or P-associated particles may be related to leaky or tight TJ, respectively. Our results are not consistent with this hypothesis. However, compositional differences of CLs may be more responsible for morphological and functional of TJs. C-terminus of CL-1 binds to N-terminus of ZO-1, and C-terminus of ZO-1 binds to actin cytoskeketon. To understand the role of this binding between CL-1 and ZO-1, C-terminus mutated claudin-1 which could not bind to ZO-1 was introduced into MDCK cells. Introduced CL-1 formed ectopic TJ strands in laterral plasma membrane. This suggest that the binding between CL-1 and ZO-1 may be important to form TJ strand at the most apical site of lateral plasma membrane.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] J.Kobayashi et al.: "Formation of tight junction strands by expression of claudin-1 mutants in their ZO-1 binding site in MDCK cells"Histochem Cell Biol. 117. 29-39 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Koga et al.: "Overexpression of Polycomb-group gene rae28 in cardiomyocytes does not complement abnormal carciac morphogenesis in mice lacking rae28 but causes dilated cardiomyopathy"Laboratory Investigation. 82. 375-385 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Doiguchi et al.: "Complimentary DNA cloning and characterization of rat spergen-1, a spermatogenic cell-specific gene-1, containing a mitochondria-targeting signal"Biol.Reprod.. 66. 1462-1470 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Doiguchi et al.: "Spergen-1 might be an adhesive molecule associated with mitochondria in the middle piece of spermatozoa"Develop. Biol.. 252. 127-137 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Kuraoka et al.: "Molecular cloning of two guinea pig liver gap junction proteins, connexin32 and connexin26"Fukuoka Acta Medica. 93. 178-188 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 中村 桂一郎 他: "ギャップ結合による細胞性ネットワーク"電子顕微鏡. 37. 188-193 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] I.Yamanaka et al.: "Differential exprssion of major gapjunction proteins, connexins26 annd 32, in rat mammary glands during pregnancy and lactation"Histochem. Cell Biol. 115. 277-284 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Shibata et al.: "Diversity and molecular anatomy of gap junctions"Med. Electon Microsc.. 34. 153-159 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Nishii et al.: "Regulation of the epithelial-mesenchymal transformation through gap junction channels in heart development"Trends Cardiovasc Med.. 11. 213-218 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Nishitani et al.: "Full-sized RanBPM cDNA encodes a protein possessing a long stretch of praline and glutamine within the N-terminal region, comprising a large protein complex"Gene. 272. 25-33 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 柴田洋三郎: "新女性医学体系 29巻 (器官の形成と分化)"中山書店. 397 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] J Kobayashi et al.: "Formation of tight junction strands by expression of claudin-1 mutants in their ZO-1 binding site in MDCK cells"Histochem Cell Biol. 117. 29-39 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Koga et al.: "Overexpression of Polycomb-group gene rae28 in cardiomyocytes does not complement abnormal cardiac morphogenesis in mice lacking rae28 but causes dilated cardiomyopathy"Laboratory Investigation. 82. 375-385 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Doiguchi et al.: "Complimentary DNA cloning and characterization of rat spergen-1, a spermatogenic cell-specific gene-1, containging a mitochondria-targeting signal"Biol.Reprod. 66. 1462-1470 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Doiguchi et al.: "Spergen-1 might be an adhesive molecule associated with mitochondria in the middle piece of spermatozoa"Develop Biol. 252. 127-137 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Kuraoka et al.: "Molecular cloning of two guinea pig liver gap junction proteins, connexin32 and connexin26"Fukuoka Acta Medica. 93. 178-188 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] I.Yamanaka et al.: "Differential exprssion of major gap junction proteins, connexins26 and 32, in rat mammary glands during pregnancy and lactation"Histochem.Cell Biol.. 115. 277-284 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Shibata et al.: "Diversity and molecular anatomy of gap junctions"Med.Electon Microsc.. 34. 153-159 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Nishii et al.: "Regulation of the epithelial-mesenchymal transformation through gap junction channels in heart development"Trends Cardiovasc Med.. 11. 213-218 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Nishitani et al.: "Full-sized RanBPM cDNA encodes a protein possessing a long stretch of praline and glutamine within the N-terminal region, comprising a large protein complex"Gene. 272. 25-33 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Nakamura et al.: "Interstitial cells of Cajal-gap junction connected celluilar networks as pecemaker system for gastrointestinal mortility"Denshi Kenbikyo. 37. 188-193 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Shibata: "Development of the organs"Shin Josei Igaku Taikei 29. 1-397 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] J.Kobayashi et al.: "Formation of tight junction strands by expression of claudin-1 mutants in their ZO-1 binding site in MDCK cells"Histchem. Cell Biol.. 67. 29-39 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Koga et al.: "Overexpression of Polycomb-group gene rae28 in cardiomyocytes does not complement abnormal cardiac morphogenesis in mice lacking rae28 but causes dilated cardiomyopathy"Lab. Invest.. 82・4. 375-385 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Doiguchi et al.: "Complementary DNA cloning and characterization of rat spergen-1, a spermatogenic cell-specific gene-1, containing a mitochondria-targeting signal"Biol. Reprod.. 66・5. 1462-1470 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Doiguchi et al.: "Spergen-1 might be an adhesive molecule associated with mitochondria in the middle piece of spermatozoa"Dev. Biol.. 252・1. 127-137 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Kuraoka et al.: "Molecular cloning of two guinea pig liver gap junction proteins, connexin32 and connexin26"Fukuoka Acta Medica. 93・9. 178-188 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中村 桂一郎他: "ギャップ結合による細胞性ネットワーク-消化管運動ペースメーカー細胞を中心にして-"電子顕微鏡. 37・3. 188-193 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 柴田 洋三郎: "器官の形成と分化;新女性医学体系 29"中山書店. 397 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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