Topological analysis of GPI-80 in human neutrophils

Research Project

Project/Area Number	13670022
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	General anatomy (including Histology/Embryology)
Research Institution	Jichi Medical School
Principal Investigator	MATSUBARA Shigeki Jichi Medical School, Medicine, Professor, 医学部, 教授 (20209597)
Co-Investigator(Kenkyū-buntansha)	TAKAHASHI Katsumasa Gunma University, Medicine, Researcher, 医学部, 助手 (30326839)
Project Period (FY)	2001 – 2002
Project Status	Completed (Fiscal Year 2002)
Budget Amount *help	¥3,200,000 (Direct Cost: ¥3,200,000) Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000) Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywords	human neutrophils / GPI-80 / GPI-anchored protein / CD18 / intracellular granules / immunocytochemistry / cell biology / 細胞内穎粒
Research Abstract	GPI-80 is a novel GPI-anchored protein on human phagocytes that may regulate leukocyte adherence, locomotion, and extravasation. A unique intracellular compartment that contains GPI-80 is distinct from the previously characterized azurophil and specific granules. The GPI-80-containing compartments rapidly undergo a dramatic reorganization upon cell stimulation with a chemoattractant. By high-resolution immunofluorescence microscopy using ultrathin cryosections, after fMLP-stimulation, some GPI-80 molecules were co localized with beta 2 integrin molecules (CD18) on the cell surface. These results suggest that GPI-80 is physically associated with beta 2 integrin in human neutrophils.