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神経因性疼痛における非損傷ニューロンの役割

Research Project

Project/Area Number 13670032
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionHyogo College of Medicine

Principal Investigator

DAI Yi  Hyogo College of Medicine, research associate, 医学部, 助手 (20330441)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Koichi  Hyogo College of Medicine Professor, 医学部, 教授 (10212127)
TOKUNAGA Atsushi  Hyogo College of Medicine Associate Prof, 医学部, 講師 (70254521)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsNeuropathic pain / Gene expression / Dorsal root ganglion / BDNF / Substance P / NGF / GDNF / ラット / 脊髄神経
Research Abstract

We examined the effect of L5 spinal nerve ligation (SPNL), a neuropathic pain model, on the expression of EDNF in the uninjured L4 dorsal root ganglion (DRG). A significantly greater number of BDNF-immunoreactive neurons were observed in the ipsilateral L4 DRG than contralateral side 14 days after ligation. The percentage of BDNF mRNA-expressing cells of trkA cells significantly increased in the ipsilateral L4 DRG 14 days after ligation. Further, NGF content in the ipsilateral L4 DRG linearly increased. Local application of anti-NGF antibody to the L4 spinal nerve beside the L5 spinal nerve-ligation site prevented the development of thermal hyperalgesia for 5 days after ligation. These data suggest that BDNF, which increased in the uninjured L4 DRG neurons, acts as a sensory-neuromodulator in the dorsal horn and contributes to thermal hyperalgesia in this neuropathic pain model.
We also found that x the content of mRNA for substance P in uninjured trigeminal ganglion neurons increased following the transection of infraorbital nerve.
These data suggest that in neuropathic pain models there are significant alteration of expression of pain-related molecules such as BDNF or Substance P in uninjured primary afferent neurons and these changes are supposed to be important pathomechanisms of neuropathic pain.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] T, Fukuoka, E, Kondo, Y, Dai, N, Hashimoto, Noguchi: "Brain-derived neurotrophic factor increases in the uninjured dorsal root ganglion neurons in selective spinal nerve ligation model"J. Neurosci.. 21. 4891-4900 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T, Fukuoka, A, Tokunaga, T, Tachibana, Y, Dai, H, Yamanaka, K, Noguchi: "VR1, but not P2X3, increases in the spared L4 DRG in rats with L5 spinal nerve ligation"Pain. 99. 111-120 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y, Dai, K, Iwata, T, Fukuoka, E, Kondo, A.Tokunaga, H, Yamanaka, T, Tachibana, Y, Liu, K, Noguchi: "Phosphorylation of extracellular signal-regulated kinase in primary afferent neurons by noxious stimuli and its involvement in peripheral sensitization"J. Neurosci. 22, 7737-77452, 2002. 22. 7737-7745 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T, Fukuoka, K, Noguchi: "Contribution of the spared primary afferent neurons to the pathomechanisms of neuropathic pain"Molecular Neurobiology. 26. 57-37 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K, Obata, H, Yamanaka, T, Fukuoka, Y, Dai, A, Tokunaga, N, Hashimoto, H, Yoshikawa, K, Noguchi: "Contribution of injured and uninjured DRG neurons to pain behavior and the changes in gene expression following chronic constriction injury of the sciatic nerve in rats"Pain. 101. 65-77 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K, Tsuzuki, T, Fukuoka, M, Sakagami, K, Noguchi: "Increase of preprotachykinin mRNA in the uninjured mandibular neurons after rat infraorbital nerve transection"Neurosci. Lett,. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tetsuo Fukuoka, Eiji Kondo, Yi Dai, Norio Hashimoto, and Koichi Noguchi: "Brain-derived neurotrophic factor increases in the uninjured dorsal root ganglion neurons in selective spinal nerve ligation model"J.Neurosci. 21. 4891-4900 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tetsuo Fukuoka, Atsushi Tokunaga, Toshiya Tachibana, Yi Dai, Hiroki Yamanaka, Koichi Noguchi: "VR1, but not P2X3, increases in the spared L4 DRG in rats with L5 spinal nerve ligation"Pain. 99. 111-120 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yi Dai, Koichi Iwata, Tetsuo Fukuoka, Eiji Kondo, Atsushi Tokunaga, Hiroki Yamanaka, Toshiya Tachibana, Yi Liu and Koichi Noguchi: "Phosphorylation of extracellular signal-regulated kinase in primary afferent neurons by noxious stimuli and its involvement in peripheral sensitization"J.Neurosci. 22. 7737-7745 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tetsuo Fukuoka and Koichi Noguchi: "Contribution of the spared primary afferent neurons to the pathomechanisms of neuropathic pain"Molecular Neurobiology. 26. 57-67 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koichi Obata, Hiroki Yamanaka, Tetsuo Fukuoka, Dai Yi, Atsushi Tokunaga, Norio Hashimoto, Hideki Yoshikawa, Koichi Noguchi: "Contribution of injured and uninjured DRG neurons to pain behavior and the changes in gene expression following chronic constriction injury of the sciatic nerve in rats"Pain. 101. 65-77 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kenzo Tsuzuki, Tetsuo Fukuoka, Masafumi Sakagami and Koichi Noguchi: "Increase of preprotachykinin mRNA in the uninjured mandibular neurons after rat infraorbital nerve transection"Neurosci Lett. (In Press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Obata, H.Yamanaka, Y.Dai, T.Tachibana, T.Fukuoka, A.Tokunaga, H.Yoshikawa, K.Noguchi: "Differential activation of ERK in primary afferent neurons regulates BDNF expression following peripheral inflammation and nerve injury"J. Neurosci. (In Press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Kominato, T.Tachibana, Y.Dai, H.Tsujino, S.Maruo, K.Noguchi: "Changes in phosphorylation of ERK and Fos expression in dorsal horn neurons following noxious stimulation in a rat model of neuritis of the nerve root"Brain Res. (In Press).

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Obata, H.Yamanaka, T.Fukuoka, Y.Dai, A.Tokunaga, N.Hashimoto, H.Yoshikawa, K.Noguchi: "Contribution of injured and uninjured LRG neurons to pain behavior and the changes in gene expression following chronic constriction injury of the sciatic nerve in rats"Pain. 101. 65-77 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Dai, K.Iwata, T.Fukuoka, E.Kondo, A.Tokunaga, H.Yamanaka, T.Tachibana, Y.Liu, K.Noguchi: "Phosphorylation of extracellular signal-regulated kinase in primary afferent neurons by noxious stimuli and its involvement in peripheral sensitization"J. Neurosci.. 22. 7737-7745 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Fukuoka, A.Tokunaga, T.Tachibana, Y.Dai, H.Yamanaka, K.Noguchi: "VRT, but not P2X3, increases in the spared L4 DRG in rats with L5 spinal nerve ligation"Pain. 99. 111-120 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Liu, Y., Tachibana, T., Dai, Y., Kondo, E., Fukuoka, T., Yamanaka, H., Noguchi, K.: "Heme Oxygenase-1 Expression Following Spinal Cord injury ; the Induction in Activated Neutrophils"J. Neurotrauma. 19. 479-490 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Dai Y, Kondo E, Fukuoka T, Tokunaga A, Miki K, Noguchi K.: "The effect of electroacupuncture on pain behaviors and noxious stimulus-evoked Fos expression in a rat model of neuropathic pain"J Pain. 2(3). 151-159 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fukuoka T, Kondo E, Dai Y, Hashimoto N, Noguchi K.: "Brain-derived neurotrophic factor increases in the uninjured dorsal root ganglion neurons in selective spinal nerve ligation model"J Neurosci. 21(13). 4891-4900 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Dai, Y., Iwata, K., Kondo, E., Morimoto, T., Noguchi, K.: "A selective increase in Fos expression in spinal dorsal horn neurons following graded thermal stimulation in rats with experimental mononeuropathy"Pain. 90. 287-296 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hashimoto N, Yamanaka H, Fukuoka T, Dai Y, Obata K, Mashimo T, Noguchi K.: "Expression of HGF and cMet in the peripheral nervous system of adult rats following sciatic nerve injury"Neuroreport. 12(7). 1403-1407 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuzuki K, Kondo E, Fukuoka T, Dai Y., Tsujino H, Sakaganii M, Noguchi K.: "Differential regulation of P2X(3) mRNA expression by peripheral nerve injury in intact and injured neurons in the rat sensory ganglia"Pain. 91. 351-360 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kondo, E., Iwata, K., Ogawa, A., Tashiro, A., Tsuboi, Y., Fukuoka, T., Yamanaka, H., Dai, Y., Morimoto, T., Noguchi, K.: "Involvement of glutamate receptors on hyperexcitability of wide dynamic range neurons in the gracile nucleus of the rats with experimental mononeuropathy"Pain. 95. 153-163 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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