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Involvement of neuropeptides in the hypothalamus and limbic system in the adaptation of stress response

Research Project

Project/Area Number 13670076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

NAGATA Shoji  University of Occupational and Environmental Health, Institute of Industrial Ecological Sciences, Professor, 産業生態科学研究所, 教授 (10108722)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsstress response / hypothalamic paraventricular nucleus / hypothalamic-pituitary-adrenal axis / adaptation / c-fos gene expression / neuropeptides / immunity / corticosterone / immediate early gene (IEG) / ストレス反応 / 内分泌 / 免疫細胞 / immediate early gene / 新奇ストレス
Research Abstract

In order to clarify the processes of adaptation to stress in hypothalamic pituitary adrenal (HPA) axis and neuroendocrinoimmune system, three experiments were performed using Wister male rats.
The results were following.
1. A long-duration restraint stress for 16 hours evoked adaptive change, characterized by transient increase and gradual recovery to basal level in c-fos mRNA/Fos protein expression in the hypothalamic paraventricular nucleus (PVN) and in plasma adrenocorticotropin (ACTH) levels, and transient decrease following slow recovery in a number of total white blood cells and some lymphocytes subpopulations, although circulating corticosterone (CORT) remained at high level.
2. Superimposed cold stress-induced re-expression of c-fos mRNA/Fos in the parvocellular region of the PVN was observed in 16 hours restraint rats. The degree of expression of c-fos gene in superimposed cold stress rats was the same as those observed in single cold stress rats (normo-responsive). The plasma AC … More TH levels in superimposed cold stress rats were significantly higher compared with those in single cold stress rats (hyper-responsive, facilitation). There was no significant differences between plasma CORT level s superimposed cold stress rats and those in single cold stress rats (not hyper-responsive).
3. The plasma ACTH and CORT levels, c-fos mRNA and AVP heteronuclear RNA expression in the PVN in the repeated restraint stress (1 hour/day) rats showed the decreasing tendency (habituation) at 7 days and 14 days later comparing with those at the first experiment day. On the other hand, the habituation of these responses was not observed in repeated cold (4℃)+restraint stress rats.
These results suggest that adaptation of stress response may occur in various stressful situation and the stress response to a superimposed stressor may occur in a different way at each level of the HPA axis, and that occurrence of habituation or facilitation of stress response to repeated stressors may related the sort of stressors. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Nagata, S: "Needs for comprehensive stress management system in the workplace"THE JOURNAL OF TOKYO MEDICAL UNIVERSITY. 58・3. 422-427 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Irie, M: "Classical conditioning of oxidative DNA damage in rats"Neuroscience Letters. 288. 13-16 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Irie, M: "Relationships between perceived workload, stress and oxidative DNA damage"Int Arch Occup Environ Health. 74. 153-157 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Irie, M: "Can conditioned histamine release occur under urethane anesthesia in guinea pigs?"Physiology & Behavior. 72. 567-573 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Irie, M: "Psychosocial factors as a potential trigger of oxidative DNA damage in human leukocytes"Jpn.J.Cancer Res.. 92. 367-375 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurose, T: "Nociceptive stimulation increases NO synthase mRNA and vasopressin heteronuclear RNA in the rat paraventricular nucleus"Autonomic Neuroscience : Basic and Clinical. 88・1-2. 52-60 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata, S: "Current status and new trends in the promotion of occupational mental health"J UOEH. 24(supp). 61-70 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurose, T: "Effect of restricted feeding on the activity of hypothalamic orexin(OX)-A containing neurons and OX2 receptor mRNA level in the paraventricular nucleus of rats"Regulatory Peptides. 104・15. 145-151 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tan, Z: "PVN c-fos expression, HPA axis response and immune cell distribution during restraint stress"J UOEH. 24・2. 131-149 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tan, Z: "Superimposed cold stress-induced hypothalamic-pituitary-adrenal response during long-duration restraint stress"J UOEH. 24・4. 361-373 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata, S., Mishima, N., Kubota, S.: "Needs for comprehensive stress management system in the workplace"THE JOURNAL OF TOKYO MEDICAL UNIVERSITY. 58(3). 422-427 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Irie, M., Asami, S., Nagata, S., Miyata, M., Kasai, H.: "Classical conditioning of oxidative DNA damage in rats"Neuroscience Letters. 288. 13-16 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Irie, M., Asami, S., Nagata, S., Miyata, M., Kasai, H.: "Relationships between perceived workload, stress and oxidative DNA damage"Int Arch Occup Environ Health. 74. 153-157 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Irie, M., Maeda, M, Nagata, S.: "Can conditioned histamine release occur under urethane anesthesia in guinea pigs?"Physiology & Behavior. 72. 567-573 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Irie, M., Asami, S., Nagata, S., Ikeda, M., Miyata, M., Kasai, H.: "Psychosocial factors as a potential trigger of oxidative DNA damage in human leukocytes"Jpn. J. Cancer Res.. 92. 367-375 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurose, T., Ueta, Y., Nomura, M., Yamaguchi, K., Nagata, S.: "Nociceptive stimulation increases NO synthase mRNA and vasopressin heteronuclear RNA in the rat paraventricular nucleus"Autonomic Neuroscience: Basic and Clinical. 88 (1-2). 52-60 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Irie, M., Asami, S., Nagata, S., Miyata, M., Kasai, H.: "Psychological mediation of a type of oxidative DNA damage, 8-Hydroxydeoxyguanosine, in peripheral blood leukocytes of non-smoking and non-drinking workers"Psychother Psychosom. 71. 90-96 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata, S., Mishima, N., Kubota, S.: "Current status and new trends in the promotion of occupational mental health"J UOEH. 24 (supp). 61-70 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurose, T., Ueta, Y., Yamamoto, Y., Serino, R., Ozaki, Y., Saitoh, J., Nagata, S., Yamashita, H.: "Effects of restricted feeding on the activity of hypothalamic orexin (OX)-A containing neurons and OX2 receptor mRNA level in the paraventricular nucleus of rats"Regulatory Peptides. 104(15). 145-151 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tan, Z., Nagata, S.: "PVN c-fos expression, HPA axis response and immune cell distribution during restraint stress"J UOEH. 24(2). 131-149 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tan, Z., Nagata, S.: "Superimposed cold stress-induced hypothalamic-pituitary-adrenal response during long-duration restraint stress"J UOEH. 24(4). 361-373 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurose T: "Nociceptive stimulation Increases NO synthase mRNA and vasopressin heteronuclear RNA in the rat paraventricular nucleus"Autonomic Neuroscience : Basic and Clinical. 88・1-2. 52-60 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 入江正洋: "ストレスと免疫"診断と治療. 89・5. 783-791 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kurose T: "Effects of restricted feeding on the activity of hypothalamic orexin (OX) -A containing neurons and OX2 receptor mRNA level in the paraventricular nucleus of rats"Regulatory Peptides. 104・15. 145-151 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tan Z: "PVN c-fos expression, HPA axis response and immune cell distribution during restraint stress"J UOEH. 24・2. 131-149 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tan Z: "Superimposed cold stress-induced hypothalamic-pituitary-adrenal response during long-duration restraint stress"J UOEG. 24・4. 361-373 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kurose, T., Ueta, Y., Nomura, M., Yamaguchi, K., Nagata, S.: "Nociceptive stimulation Increases NO synthase mRNA and vasopressin heteronuclear RNA in the rat paraventricular nucleus"Autonomic Neuroscience : Basic and Clinical. 88・1-2. 52-60 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Irie, M., Maeda, M., Nagata, S.: "Can conditioned histamine release occur under urethane anesthesia in guinea pigs?"Physiology & Behavior. 72. 567-573 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 入江正洋, 永田頌史: "ストレスと免疫"診断と治療. 89・5. 783-791 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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