| Project/Area Number |
13670077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
KANAI Setuko Tokyo Metro. Inst. Gerontol. Head of Dept. Clin. Physiol., ASSISTANTS RESEARCHER, 東京都老人総合研究所・生体機能調節と加齢グループ, 研究助手 (90100122)
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| Co-Investigator(Kenkyū-buntansha) |
MIYASAKA Kyouko Tokyo Metro. Inst. Gerontol. Head of Dept. Clin. Physiol., HEAD, 福祉振興財団東京都老人総合研究所・生体機能調節と加齢グループ, グループリーダー (90166140)
増田 正雄 東京都老人総合研究所, 臨床生理部門, 研究助手 (20260284)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | CCK receptor / CCK-B receptor / CCK-A receptor / pancreas / gene / gene expression / gallstone / CCK-A受容体KOマウス / CCK-B受容体KOマウス / CCK-A, B受容体ダブルKOマウス |
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| Research Abstract |
Two types of CCK receptors (type A and type B) have been identified. We raised CCK-A,-B and AB receptor knockout mice, the role of these receptors in gastric functions and bile-pancreatic secretions was determined.
1. Basal gastric acid secretion was decreased in mice lacking CCK-BR. Administration of gastrin and histamine significantly increased acid secretion in all genotypes.
2. Lack of CCK-BR enhanced gastric emptying of a liquid load. The gastric emptying was inhibited by administration of CCK in mice with CCK-AR, but not in mice without CCK-AR. Atropine inhibited gastric emptying in all genotypes, however, larger doses were required to reveal a similar effect in mice with CCK-BR to in mice without CCK-BR. Therefore, autonomic nerve functions might be altered by the lack of CCK-BR
3. We confirmed that CCK did not induce gallbladder contraction nor pancreatic amylase secretion in CCK-AR(-/-) mice. Other stimulants such as acethocholine and neuromedin C could stimulated amylase secretion in CCK-AR(-/-) mice but could not produce gallbladder contraction. Secretin failed to increase bicarbonate secretion in mice, and CCK increased bicarbonate secretion via CCK-A receptor in mice. Glucose tolerance was not altered in CCK-AR(-/-) mice.
4. Age associated gallstone formation was induced by administration of a higher protein and fat diet in CCK-AR(-/-) mice, but not in wild type mice.
5. Anxiety related behaviors in the plus maze were increased in mice without CCK-BR.
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