| Project/Area Number |
13670082
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Shinshu University |
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Principal Investigator |
CHIBA Shigetoshi Shinshu Univ., Sch. Med., Professor, 医学部, 教授 (30004659)
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| Co-Investigator(Kenkyū-buntansha) |
弘瀬 雅教 信州大学, 医学部, 助手 (40273081)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Isolated canine right atria / Sinus node artery / Blood-perfused sinus node preparation / Adenosine / Desensitization / Intracardiac nerve stimulation / イヌ洞結節動脈 / ノルアドレナリン / アセチルコリン / 心臓内自律神経刺激 |
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| Research Abstract |
After the canine heart was isolated under anesthesia with anticoagulant treatment, the right at rial preparation containing the sinus node area was prepared. With the anesthetized donor dog's heparinized blood, the sinus node artery of the isolated atrium was perfused at the 100 mmHg of constant pressure.
A continuous infusion of adenosine caused negative chrono- and inotropic effects, and the contractility gradually returned to the control level but the pacemaker activity did not within the infusion. 1. Responses of pacemaker activity and contractility to adenosine are differentiated. The responses were dose-dependent. 2. The adenosine infusion inhibited a bolus administration of adenosine, showing the acute desensitization phenomenon. However, they did not inhibit the acetylcholine-induced response but rather potentiated that. 3. The adenosine infusion slightly but significantly inhibited the norepinephrine-induced positive chrono-and inotropic effects. 4. The biphasic negative and positive chrono- and inotropic responses to intracardiac nerve electric stimulation caused to enhance the negative cardiac but to depress the positive responses.
In the present experiments, it was confirmed that adenosine influences physiological control factors, i.e., adenosine-, acethylcholine-, norepinephrine-induced cardiac effects. In the future, it is necessary to investigate the pathophysiological and pharmacological problems for adenosine.
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