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Determination of aPKC substrates in the regulation of cell polarity.

Research Project

Project/Area Number 13670129
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionYokohama City University

Principal Investigator

MIZUNO Keiko  Yokohama City Univ., Sch.of Medicine, assistant instructor, 医学部, 助手 (90221803)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
KeywordsCell polarity / atypical PKC / PAR-3 / ASIP
Research Abstract

Atypical protein kinase C (aPKC)-PAR complex is evolutionary conserved proteins and play essential roles on the establishment of cell polarity. In mammalian epithelial cells, aPKC co-localizes with PAR-3/ASIP and PAR-6 to the tight junction (TJ) and is essential for the establishment of TJ, although molecular mechanism regulating cell polarity remains unknown. One of the aPKC-binding proteins, 14-3-3/PAR-5 has been reported to be involved in the regulation of cell polarity in C. elegance embryo or Drosophila. In this study, I demonstrated that mammalian PAR-3 could bind to 14-3-3 in phosphorylation dependent manner. I also determined the Ser rsidue of PAR-3 subject to specific antibody demonstrated the phosphorylation of this Ser residue in polarized MDCK cells. In residue of GST-PAR-3 as substrate. These results suggest that aPKC phosphorylates this Ser on PAR-3 and regulates the binding of PAR-3 and 14-3-3/PAR-5.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Nagai-Tamai Y: "Regulated protein-protein interaction between aPKC and PAR-3 plays an essential role in the polarization of epithelial cells."Genes to Cells. 7. 1161-1171 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mishima A: "Overexpression of PAR-3 suppresses contact-mediated inhibition of cell migration in MDCK cells."Genes to Cells. 7. 581-596 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirose S: "Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction fomation."J.Cell Sci.. 115. 2485-2495 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kitamura K: "The second phase activation of protein kinase Cd at late G1 is required for DNA synthesis in serum-induced cell cycle progression."Genes to Cells. 8. 311-324 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuno K: "Self-association of PAR-3 mediated by the conserved N-terminal domain contributes to the development of epithelial tight junctions."J.Biol.Chem.. 278. 31240-31250 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 西田栄介, 大野茂男: "バイオサイエンスの新世紀9 シグナル伝達-細胞運命と細胞機能を制御する仕組み-"共立出版社. 204 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nagai-Tamai Y, et al.: "Regulated protein-protein interaction between aPKC and PAR-3 plays an essential role in the polarization of epithelial cells."Genes to Cells. 7. 1161-1171 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mishima A, et al.: "Overexpression of PAR-3 suppresses contact-mediated inhibition of cell migration in MDCK cells."Genes to Cells. 7. 581-596 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirose S, et al.: "Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction fomation."J.Cell Sci.. 115. 2485-2495 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kitamura K, et al.: "The second phase activation of protein kinase Cd at late G1 is required for DNA synthesis in serum-induced cell cycle progression."Genes to Cells. 8. 311-324 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuno K, et al.: "Self-association of PAR-3 mediated by the conserved N-terminal domain contributes to the development of epithelial tight junctions."J.Bio.Chem.. 278. 31240-31250 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kitamura, K: "The second phase activation of protein kinase Cδ at late G1 is required for DNA synthesis in serum-induced cell cycle progression"Genes to Cells. 8. 311-324 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mizuno, K: "Self-association of PAR-3 mediated by the conserved N-terminal domain contributes to the development of epithelial tight junctions"J.Biol.Chem.. 278. 31240-31250 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nagai-Tamai, Y.: "Regulated protein-protein interaction between aPKC and PAR-3 plays an essential role in the polarization of epithelial cells"Genes to Cells. 7(11). 1161-1171 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mishima, A.: "Over-expression of PAR-3 suppresses contact-mediated inhibition of cell migration in MDCK cells"Genes to Cells. 7(6). 581-596 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirose, S.: "Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation"J Cell Sci.. 115. 2485-2495 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, A.: "Atypical PKC is involved in the establishment of epithelial polarity in mammalian cells"J. Cell Biol.. 152. 1183-1196 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takaki, Y.: "Dynamic changes in protein components of the tight junction during liver regeneration."Cell Tissue Res.. 305. 399-409 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 西田栄介, 大野茂男: "バイオサイエンスの新世紀9 シグナル伝達-細胞運命と細胞機能を制御する仕組み-"共立出版. 204 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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