Histopathological re-evaluation of pancreatic neoplasms : Special reference to immunohistochemistry and molecular biology

• Project/Area Number: 13670159
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Saitama Medical School
• Principal Investigator: SHIMIZU Michio Saitama Medical School, Professor, 医学部, 教授 (60226256)
• Co-Investigator(Kenkyū-buntansha): ITOH Tomoo Hokkaido University, Graduate School of Medicine, Assistant Professor, 医学部, 講師 (20301880)
• Project Period (FY): 2001 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,600,000); Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: immunohistochemistry / IPMN / PanIN / pancreatic cancer / MUC1 / p53 / MIB-1 / intraglandular necrotic debris / MUC2 / ductal adenocarcinoma / endocrine tumor / CEA / CA19-9 / VEGF / HE染色標本 / intraductal papillary-mucinous tumor / p27 / MMP-7 / E-cadherin

Research Abstract

We performed the histopathological re-evaluation of pancreatic neoplasms (including intraductal papillary mucinous neoplasms (IPMN), endocrine tumors, pancreatic intraepithelial neoplasia (PanIN), pancreatic cancers, et al.) by using immunohistochemistry and molecular biology from 2001 to 2003. Then, we tried to find the important hematoxylin-eosin (HE) staining findings related to the above mentioned results.
In IPMN cases, slightly high villous architecture and nuclear overlapping were the most important findings to differentiate among intraductal papillary mucinous adenoma (IPMA), intraductal papillary mucinous neoplasm, borderline (IPMB), and intraductal papillary mucinous carcinoma (IPMC). Regarding endocrine tumors (including both benign and malignant tumors), so far there seemed to be no useful HE findings to diagnose malignant ones, although we found several cases of endocrine tumor with ductal differentiation. MUC1 was thought to be a good marker for malignancy in PanIN lesions. All cases of PanIN was negative for MUC2. On the other hand, in cases of pancreatic cancers, most cases revealed positive staining of p53 and MIB-1, and the nuclear atypia was obvious in such cases. There were several cases of well differentiated adenocarcinoma showing less atypical features. In such cases, intraglandular necrotic debris was a diagnostic clue to adenocarcinoma.

Research Products

• [Journal Article] Two major types of intraductal papillary mucinous neoplasm of the pancreas (2005)
  • Author(s): Ban S, Shimizu M, et al.
  • Journal Title: Mod Pathol 18(Supplement 1)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Detection of lymphatic invasion in pancreatic cancers based on immunohistohemistry of D2-40 (2005)
  • Author(s): Shimizu Y, Shimizu M, et al.
  • Journal Title: Mod Pathol 18(Supplement 1)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] PanINとIPMN(IPMT)の鑑別 (2005)
  • Author(s): 伴 慎一, 内藤善久, 清水道生
  • Journal Title: 胆と膵 (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] IPMNとMCNの病理診断の問題点 (2005)
  • Author(s): 伴 慎一, 内藤善久, 清水道生
  • Journal Title: 胆と膵 (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Two major types of intraductal papillary mucinous neoplasm (IPMN) of the pancreas : their distinct histological and mucin phenotypic features. (2005)
  • Author(s): Ban S, Naitoh Y, Sakurai T, Kuroda M, Shimizu M
  • Journal Title: Mod Pathol 18(Supplement 1)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Detection of lymphatic invasion in pancreatic cancers based on immunohistochemistry of D2-40. (2005)
  • Author(s): Shimizu Y, Ban S, Mitsuhashi T, Ogawa F, Hirose T, Shimizu M
  • Journal Title: Mod Pathol 18(Supplement 1)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Problems concerning histological diagnosis of IPMN and MCN. (2005)
  • Author(s): Ban S, Naitoh Y, Shimizu M
  • Journal Title: Shoukakika (in Japanese) (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Features discriminating IPMN (IPMT) from PanIN. (2005)
  • Author(s): Ban S, Naitoh Y, Shimizu M
  • Journal Title: Tan to Sui (in Japanese) (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Forum on carcinoma in situ of the pancreas. (2005)
  • Author(s): Ban S, Shimizu M, Naitoh Y
  • Journal Title: Medical Tribune (Tokyo) (in Japanese) Pages: 106-115
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An illustrated consensus on the classification of pancreatic intraepithelial neoplasia and intraductal papillary mucinous neoplasia (2004)
  • Author(s): Hruban RH, Shimizu M, et al.
  • Journal Title: Am J Surg Pathol 28 Pages: 977-987
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An illustrated consensus on the classification of pancreatic intraepithelial neoplasia and intraductal papillary mucinous neoplasms. (2004)
  • Author(s): Hruban RH, Takaori K, Klimstra DS, Adsay NV, Albores-Saavedra J, Biankin AV, Biankin SA, Compton CC, Fukushima N, Furukawa T, Goggins M, Kato Y, Kloppel G, Longnecker DS, Luttges J, Maitra A, Offerhaus GJ, Shimizu M, Yonezawa S
  • Journal Title: Am J Surg Pathol 28 Pages: 977-987
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 膵上皮内癌フォーラム (2005)
  • Author(s): 伴 慎一, 清水道生, 内藤善久
  • Publisher: メディカルトリビューン
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hruban RH, Shimizu M, et al.: "An illustrated consensus on the classification of PanIN and IPMNs"Am J Surg Pathol. (in press).