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Molecular Analysis on Tumor Invasion and Metastasis of CD44 Variant Forms

Research Project

Project/Area Number 13670163
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionChiba University

Principal Investigator

HARIGAYA Kenichi (2002)  Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (40101894)

岸 宏久 (2001)  千葉大学, 大学院・医学研究院, 助手 (90323394)

Co-Investigator(Kenkyū-buntansha) KITAGAWA Motoo  Chiba University, Graduate School of Medicine, Associate professor, 大学院・医学研究院, 助教授 (40262026)
張ケ谷 健一  千葉大学, 大学院・医学研究院, 教授 (40101894)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsTUMOR NECROSIS / TUMOR INVASION / TUMOR METASTASIS / CD44E / VEGF / MICROVESSEL FORMATION / TUMOR PROGRESSION / 腫瘍浸潤転移 / CD44E / 腫瘍のプログレッション / 腫瘍 / 浸潤 / 転移 / バリアント
Research Abstract

CD44 is a major receptor for hyaluronan and is involved in many biological processes, including cell migration, tumor invasion, and metastasis. To examine how CD44 variant molecules contribute to the tumor cell invasion and metastasis, standard CD44s (82s), CD44v8-10 (82E) and mock (82pCI) transfectants of a CD44-negative human lung cancer cell line were subcutaneously injected into SCID mice. In 82pCI- and 82s-derived tumors, multiple irregular foci of necrosis were prominent, while necrosis was scant in 82E tumors. We found the increased small vessels in the stroma of 82E tumors compared to those of 82s and 82pCI tumors. 82E cells showed the enhanced production of vascular endothelial growth factor (VEGF) in both mRNA and protein levels. The levels of the VEGF mRNA from 82E cells increased in a cell-density dependent manner, and this induced production was abolished by the treatment with hyaluronidase as the cells carried hyaluronan on their surface. Furthermore, the addition of stimulatory anti-CD44 antibody also increased the production of VEGF. These suggest that a homotypic cell-cell contact stimulates the CD44v8-10 through the engagement of cell surface hyaluronan and resulted in the enhanced production of VEGF. Accordingly, our study indicates that CD44v8-10 positive cancer may have an advantage to grow by the induced angiogenesis through the VEGF production.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Harigaya K et al.: "Interleukin-11 as a stimulatory factor for bone formation prevents bone loss with advancing age in mice"J Biol Chem. 277. 49011-49018 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harigaya K et al.: "Identification of new human mastermind proteins defines a family that consists of positive regulators for notch signaling"J Biol Chem. 277. 50612-50620 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harigaya K et al.: "CD44 signaling through focal adhesion kinase and its anti-apoptotic effect"FEBS Lett. 528. 101-108 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harigaya K et al.: "Hypermethylation of the CD44 gene is associated with progression and metastasis of human rostate cancer"Prostate. 49. 110-115 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harigaya K et al.: "A human protein with sequence similarity to Drosophila mastermind coordinates the nuclear form of notch and a CSL protein to build a transcriptional activator complex on target promoters"Mol Cell Biol.. 21. 4337-4346 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harigaya K et al.: "VH gene analysis in sporadic Burkiti's lymphoma : somatic mutation and intraclonal diversity with special reference to the tumor cells involving germinal center"Leuk Lymphoma. 43. 159-164 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeuchi Y, Watanabe S, Ishii G, Takeda S, Nakayama K, Fukumoto S, Kaneta Y, Inoue D, Matsumoto T, Harigaya K, and Fujita T: "Interleukin-11 as a stimulatory factor for bone formation prevents bone loss with advancing age in mice"J Biol Chem. 277. 49011-49018 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lin S, Oyama T, Nagase T, Harigaya K, Kitagawa M: "Identification of new human mastermind proteins defines a family that consists of positive regulators for notch signaling"J Biol Chem. 277. 50612-50620 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujita Y, Kitagawa M, Nakamura S, Azuma K, Ishii G, Higashi M, Kishi H, Hiwasa T, Koda K, Nakajima N, Harigaya K.: "CD44 signaling through focal adhesion kinase and its anti-apoptotic effect"FEBS Lett.. 528. 101-108 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isobe K, Tamaru J, Nakamura S, Harigaya K, Mikata A, Ito H: "VH gene analysis in sporadic Burkitt's lymphoma : somatic mutation and intraclonal diversity with special reference to the tumor cells involving germinal center"Leuk Lymphoma. 43. 159-164 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kito H, Suzuki H, Ichikawa T, Sekita N, Kamiya N, Akakura K, Igarashi T, Nakayama T, Watanabe M, Harigaya K, Ito H.: "Hypermethylation of the CD44 gene is associated with progression and metastasis of human prostate cancer"Prostate. 49. 110-115 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kitagawa M, Oyama T, Kawashima T, Yedvobnick B, Kumar A, Matsuno K, Harigaya K.: "A human protein with sequence similarity to Drosophila mastermind coordinates the nuclear form of notch and a CSL protein to build a transcriptional activator complex on target promoters"Mol Cell Biol.. 21. 4337-4346 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harigaya K et al.: "Interleukin-II as a stimulatory factor for bone formation prevents bone loss with advancing age in mice"J. Biol. Chem. 277. 49011-49018 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harigaya K et al.: "Identification of new human mastermind proteins defines a family that consists of positive regulators for notch signaling"J. Biol. Chem. 277. 50612-50620 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harigaya K et al.: "CD44 signaling through focal adhesion kinase and its anti-apoptotic effect"FEBS Lett. 528. 101-108 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harigaya K et al.: "Hypermethylation of the CD44 gene is associated with progression and metastasis of human prostate cancer"Prostate. 49. 110-115 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harigaya K et al.: "A human protein with sequence similarity to Drosophila mastermind coordinates the nuclear form of notch and a CSL protein to build a transcriptional activator complex on target promoters"Mol Celt Biol. 21. 4337-4346 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harigaya K et al.: "VH gene analysis in sporadic Burkitt's lymphoma : somatic mutation and intraclonal diversity with special reference to the tumor cells involving germinal center"Leuk. Lymphoma. 43. 159-164 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kito H., et all.: "Hypermethylation of the CD44 gene is associated with progression and metastasis of human prostate cancer"Prostate. 49(2). 110-115 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kitagawa M., et all.: "A human protein with sequence similarity to Drosophila mastermind coordinates the nuclear form of notch and a CSL protein to build a transcriptional activator complex on target promoters"Mol Cell Biol.. 21(13). 4337-4346 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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