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Development of new method for early detection & diagnosis of adenoma malignum of the uterin cervix

Research Project

Project/Area Number 13670168
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionShinshu University

Principal Investigator

ISHII Keiko  Shinshu University, School of Medicine, assistant, 医学部, 助手 (00252099)

Co-Investigator(Kenkyū-buntansha) SHIOZAWA Tanri  Shinshu University, School of Medicine, assistant professor, 医学部, 講師 (20235493)
NAKAYAMA Jun  Shinshu University, Graduate School of Medicine, Professor, 大学院, 教授 (10221459)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsuterine cervix / adenoma malignum / HIK1083 / latex agglutination test / gland mucus cell type mucin / α4GnT / RT-PCR / 子宮頸部 / 悪性腺種
Research Abstract

1. We evaluated the usefulness of a rapid, simple method for the diagnosis of adenoma malignum (AM) and related lesions using a latex agglutination test. Cervical mucus samples from 120 patients including 10 gastric-type cervical lesions and 5 positive controls were included in this study. We employed a latex agglutination test kit developed by Kanto Chemical. Clear agglutination confirmed its specificity was observed in only 10 gastric-type cervical lesions and all positive controles. It has demonstrated that the HIK1083-labeled latex agglutination test is highly specific for the identification of gastric mucin in the cervical mucus. This method enables us to diagnose AM and related lesions at their clinically early stage.
2. We evaluated the usefulness of the RT-PCR assay targeted to α 1,4-N-Acetylglucosaminyltransferase (α4GnT) for the detection of AM and lerated lesions, α 4GnT is a glycosyltransferase that forms a unique glycan, specifically present in gastric gland mucous cell-type mucin. To detect gastric type cancer cells circulating in the peripheral blood of AM and related lesions, we quantitatively analyzed the expression level of α4GnT mRNA in the mononuclear cell fraction of peripheral blood using RT-PCR. The transcripts of α4GnT were detected in the mononuclear cell fraction isolated from all patients but not from any of 23 healthy individuals. These results altogether indicate that the quantitative analysis of α4GnT mRNA expressed in the peripheral blood is useful for the detection and, possibly, monitoring of AM and related lesions as well as gastric cancer.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Keiko Ishii: "A new diagnostic method for adenoma malignum and related losions ; latex agglutination test with a new monoclonal antibody, HIK1083"Clinica Chimica Acta. 312. 231-233 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroyoshi Ota: "Call Lineage Specificity of Newly Raised Monoclonal Antibodies Against Gastric Mucins in Normal, Metaplastic, and Neoplastic Human Tissues and their Application to pathology diagnosis"America Journal of Clinical Pathology. 115. 69-79 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Keiko Ishii: "Histo-and cytochemistry of gland mucus cell type mucin and its practical application for pathologic and clinical diangnosis"Acta Histochem Cytochem. 35. 48 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimizu Fumiaki: "Usefulness of the real-time reverse transcription-polymerase chain reaction assay targeted to α1,4-N acetylglucosaminyltrass ferase for the detection of gastric cancer"Lab Invest. 83. 187-197 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jun Nakayama: "Handbook of Glycosyltransferases and Related Genes"Springer-Verlag. 6 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Keiko Ishii: "A new diagnostic method for adenoma malignum and relatel lesions : latex agglutination test with a new monoclonal antibody, HIK1083"Clinica Chimica Acta. 312. 231-233 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroyoshi Ota: "Cell Lineage Spcificity of Nowly Raised monoclonal Antibodies Against Gastric Mueins in Normal, metaplastic, and Neoplastic Human Tissues and their Application to pathology diagnosis"American Journal of Clinical Pathology. 115. 67-79 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Keiko Ishii: "Histo- and cytochemistry of gland mucus cell type mucin and its practical application for pathologic and clinical diagnosis"Acta Histochem cytochem. 35. 48 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fumiaki Shimizu: "Usefulness of the real-time reverse transcription-polymerase chain reaction assay targeted to α1,4-Nacetylglucosaminyltransferase for the detection of Gastric Cancer"Laboratory Inerstigation. 83. 187-197 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jun Nakayama: "α4-N-acetylilucosaminyltransferase, Handbook of Glycosyltransferase and Related Genes"Springer-Verlag. 151-157 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroyoshi Ota: "Cell Lineage Specificity of Newly Raised Monoclonal Antibodies Against Gustric Mucins in Normal, Metaplastic, and Neoplastic Human Tissues"American Journal of Clinical Pathology. 115. 69-79 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Keiko Ishii: "Histo-and cytochemistry of gland mucus cell type muchin and its practical application for pathologic and clinical diaqnosis"Acta Histochem Cytochem. 35. 48 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fumiaki Shimizu: "Usefulness of the Real-Time Reverse Transcription-Polymerase Chain Reaction Assay Targeted to α1, 4-N-Acetylglucosaminyltrasnferase for the Detection of Gastric cancer"Laboratory Investigation. 83. 187-197 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Jun Nakayama: "Hundbook of Glycosyltransferases and Related Genes"Springer-Verlag. 6 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Keiko Ishii: "A new diagnostic method for adenoma malignum and related lesions : latex agglutination test with a new monoclonal antibody, HIK1083"Clinica Chimica Acta. 312. 231-233 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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