| Project/Area Number |
13670174
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KAWAUCHI Shigeto Yamaguchi University School of Medicine, Associate Professor, 医学部, 講師 (80284511)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Kohsuke Yamaguchi University School of Medicine, Professor, 医学部, 教授 (80116722)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | in situ RT-PCR / messenger RNA / vascular endothelial growth factor / osteopontin / synovial sarcoma / chimera gene / p27 / prognosis / in situ RT-PCR / パラフィン包埋組織 / 非特異的標識 |
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| Research Abstract |
In situ reverse transcriptase-polymerase chain reaction (in situ RT-PCR) is a newly developed technique that allows in situ amplification of reverse-transcribed complementary DNA (cDNA) originating from mRNA. The technique is considered to be more suitable for in situ detection of target mRNA in routinely processed formalin-fixed paraffin-embedded tissue sections than in situ hybridization. We developed convenient in situ RT-PCR techniques for osteopontin and vascular endothelial growth factor mRNAs. Furthermore, synovial sarcoma, which is characteristic of chimeric SYT-SSX gene, is proved to express the chimeric transcript in all tumor cells by in situ RT-PCR. A significant inverse correlation between p27 expression and apoptosis was demonstrated in synovial sarcoma. A combination of p27 expression and apoptosis allowed more accurate estimation of prognosis of patients with synovial sarcoma than each parameter alone
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