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Analysis of gene expressions involved in cancer-host cells cross talk at the invasion front of nonsmall cell lung cancer

Research Project

Project/Area Number 13670192
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionKANAZAWA MEDICAL UNIVERSITY

Principal Investigator

UEDA Yoshimichi  Kanazawa Medical University, Department of Medicine, Professor, 医学部, 教授 (50271375)

Co-Investigator(Kenkyū-buntansha) NAMBU Yoshihiro  Kanazawa Medical University, Department of Medicine, Lecturer, 医学部, 講師 (20237639)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsLung cancer / Invasion and metastasis / mRNA profiling / cDNA macroarray / Real time RT-PCR / Laser-captured microdissection / FDG-PET / MIBI-PET / リアルタイムRT-PCR法 / Laser microdissection法 / 定量RT-PCR法 / Jagged-1 / Villin 2 / DNAマクロアレイ / プログレッション / in situ gelatin zymography
Research Abstract

It is recently proposed that cancer cells make microenvironment suitable for their invasion through cross talk with host cells. To elucidate genes or molecules involved in the cancer cell-host cell cross talk at the invasion front of nonsmall cell lung cancer (NSCLC), mRNA profilings of 1176 cancer-related genes at the invasion front tissues of NSCLCs of various progressions were analyzed by cDNA macroarray system (Cancer array 1.2, Clontech). 121 genes, including cyclin D1, c-myc, insulin-like growth factor, rho-associated GTP binding proteins, laminin receptor, DNA polymerases, beta-catenin, wnt 8B, elongation factor alpha-1, were commonly overexpressed in NSCLC tissues. Ten genes, such as membrane-type 1 matrix metalloproteinase (MT1-MMP), MMP-3, p21-rac1, vascular endothelial growth factor (VEGF), jagged-1, jagged-2, notch-4, c-fos related antigen, were overexpressed both in squamous cell carcinomas and high stage-adenocarcinomas compared with low stage-adenocarcinomas, while ezrin (villin 2) and macrophage inhibitory cytokine (MTC)-1 genes did the opposite. These results were confirmed by real time RT-PCR using TaqMann-probe method. These data suggested that rac-1/MT1-MMP, jagged-1, -2/ notch-4, p53 / MIC-1 groups may actively involved in the cross talk between lung cancer and host cells and works either positively or negatively to make appropriate microenvironment for invasion of the lung cancer cells. We have also established laser-captured microdissection / real time RT-PCR method in order to analyze gene expressions more in detail at the invasion front of NSCLCs. Furthermore, functional and non-invasive examination methods of gene expressions in NSCLC tissues in vivo were tried successfully using FDG- and MIBI-PET. Present results may contribute to estimate biological features of NSCLCs and establish adequate therapy for lung cancer patients.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Jinshan Zhou et al.: "Expression of multidrug resistence protein and mRNA correlate with 99mTc-MIBI imaging in patients with lung cancer"J. Nucl. Med.. 42・10. 1476-1483 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kotaro Higashi et al.: "Value of whole-body FDG PET in management of lung cancer"Ann. Nucl. Med.. 17・1. 1-14 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jinshan Zhou, Kotaro Higashi, Yoshimichi Ueda, Yuko Kodama, Dachuan Guo, Fumiko Jisaki, Aya Sakurai, Tsutomu Takegami, Shogo Katsuda, and Itaru Yamamoto: "Expression of multidrug resistance protein and messenger RNA correlate with 99mTc-MIBI imaging in patients with lung cancer."J. Nucl. Med.. 42(10). 1476-1483 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kotaro Higashi, Ichiro Matsunari, Yoshimichi Ueda, Ryosuke Ikeda, Jinfei Guo, Manabu Oguchi, Hisao Tonami and Itaru Yamamoto: "Value of whole-body FDG PET in management of lung cancer."Ann. Nucl. Med.. 17(1). 1-14 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jinshan Zhou: "Expression of multidrug resistence protein and mRNA correlate with 99mTc-MIBI imaging in patients with lung cancer"J. Nuci. Med.. 42・10. 1476-1483 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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