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Molecular pathological study on tumorigenesis of beta-catenin gene alterated foci in rat colon mucosa

Research Project

Project/Area Number 13670208
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionUniversity of the Ryukyus

Principal Investigator

YOSHIMI Naoki  YOSHIMI,Naoki, 医学部, 教授 (30166996)

Co-Investigator(Kenkyū-buntansha) MORIOKA Takamitsu  University of the Ryukyus, Faculty of Medicine, Associate, 医学部, 助手 (70253961)
山田 泰広  岐阜大学, 医学部, 助手 (70313872)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsRat / Colon Carcinogenesis / beta-catenin / Chemoprevention / Preneoplastic lesions / 前癌病変 / シクロオキシゲナーゼ
Research Abstract

Although beta-catenin accumulated crypts(BCAC)is an useful biomarker in the retrieval of the chemopreventive agents on rat colon carcinogenesis, the detection of BCAC is complicated, since the preparation manufacture is needed.In this study, it was examined the mucin or mucoprotein in neoplastic lesions using the High-iron diamine alcian blue(HID+AB) PH2.5 and AB pH1.0 staining for dissolving these problems.Negative lesions by the HID+AB pH2.5 staining were significantly included in BCAC, further than the aberrant crypt foci(ACE), that is conventional lesions in rat colon carcinogenesis.Especially, the rate of concordance between negative lesion nest HID+AB pH2.5 staining and BCAC which consist of crypts over 4 was high.Therefore, the negative lesion, which consisted of crypts over 4, was able to seem to become the substitution of BCAC.However, the detection of BCAC in the proximal colon was difficult for the HID+AB pH2.5 staining because of the characteristics of the histological feature of proximal colon and the HLD+AB pH2.5 staining. Thus, we examined the possibility of the application of AB pH1.0 alone staining by which sulfomucin and sialomucin can be simultaneously represented as well as HJD+AB pH2.5 staining.The AB pH1.0 alone staining was able to detect the negative lesions easier than HID+AB pH2.5 staining, and it was also proven that the detection was possible on those in the proximal colon.In conclusion, both HID+AB pH2.5 staining and AB pH1.0 alone staining were detectable the conventional preneoplastic lesions such as BCAC and ACF.In the future, it is necessary to search the mutual relationship among these negative lesions, BCAC and ACF.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Shimizu, M., Yoshimi, N et al.: "No involvement of β-catenin gene mutation in gastric carcinomas induced by N-methyl-N-nitrosourea in male F344 rats."Cancer Letter. 195. 147-152 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Katayama M, Yoshimi, N. et al.: "Preventive effect of fermented brown rice and rice bran on diethylnitrosoamine and phenobarbital-induced hepatocarcinogenesis in male F344 rats."Oncology Reports. 10. 875-880 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimizu, M., Yoshimi, N.et al.: "No involvement of β-catenin gene mutation in gastric carcinomas induced by N-methyl-N-nitrosourea in male F344 rats."Cancer Letter. 195. 147-152 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Katayama M, Yoshimi, N. et al.: "Preventive effect of fermented brown rice and rice bran on diethylnitrosoamine and phenobarbital-induced hepatocarcinogenesis in male F344 rats."Oncology Reports. 10. 875-880 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimizu, M., Yoshimi, N.et al.: "No involvement of β-catenin gene mutation in gastric carcinomas induced by N-methyl-N-nitrosourea in male F344 rats."Cancer Letter. 195. 147-152 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Katayama M, Yoshimi, N. et al.: "Preventive effect of fermented brown rice and rice bran on diethylnitrosoamine and phenobarbital-induced hepatocarcinogenesis in male F344 rats."Oncology Reports. 10. 875-880 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kanamori T, Itoh M, Yoshimi N: "Pressure dye-spray : a simple and reliable method for differeintiating adenomas from hyperplastic polyps in the colon"Gastrointest.Endosc.. 55. 695-700 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Katayama M, Yoshimi N et al.: "Preventive effect of fermented brown rice and rice bran against colon carcinogenesis in male F344 rats"Oncology Reports. 9. 817-822 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsunaga K, Yoshimi N et al.: "Inhibitory effects of chlorogenic acid on azoxymethane-induced colon carcinogenesis in male F344 rats"Asian Pacific J Cancer Prev.. 3(2). 163-166 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mori H, Yoshimi N et al.: "Chemoprevention of large bowel carcinogenesis ; the role of control of cell proliferation and significance of β-catenin-accumulated crypts as a new biomarker"Euro.J.Cancer prevention. 11(suppl 2). S271-S275 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamada Y, Yoshimi N et al.: "Microadenomatous lesions involving loss of Apc heterozygosity in the colon of adult ApcMin/+ mice"Cancer Res.. 62. 6367-6370 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Zheng Q., Yoshimi N.: "Further investigation of the modifying effect of various chemopreventive agents on apoptosis and cell proliferation in human colon cancer cells"J.Cancer Res.Clin.Oncol.. 128. 539-546 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshimi N, Matsunaga K, et al.: "The inhibitory effects of mangiferin, a naturally occurring glucosylxanthone, in bowel carcinogenesis of male F344 rats."Cancer Lett.. 163・2. 163-170 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shimizu M, Yoshimi N, et al.: "Mechanism of retarded liver regeneration in plasminogen activator-defient mice : impaired activation of hepatocyte growth factor after Fas-mediated massive hepatic apoptosis."Hepatology. 33. 569-576 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamada Y, Yoshimi N, et al.: "Sequential analysis of morphological and biological properties of β-catenin-accumulated crypts, provable premalignant lesions independent of aberrant crypt foci in rat colon carcinogenesis"Cancer Res.. 61. 1874-1878 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamada Y, Yoshimi N, et al.: "Suppression of occurrence and advancement of β-catenin-accumulated crypts, possible premalignant lesions of colon cancer, by selective cyclooxygenase-2 inhubitor, Celecoxib."Jpn J Cancer Res.. 92. 617-623 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kiguchi K, Yoshimi N, et al.: "Constitutive Expression of ErbB-2 in Gallbladder Epithelium Results in Development of Adenocarcinoma"Cancer Res.. 61. 6971-6976 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mori H, Yoshimi N, et al.: "Cell proliferation in cancer prevention ; effects of preventive agents on estrogen-related endometrial carcinogenesis model and on an in vitro model in human colorectal cells."Mutat. Res.. 48. 201-207 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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