| Project/Area Number |
13670228
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
FUJIKAWA Kohzaburo KANAZAWA MEDICAL UNIVERSITY, Research Institute of Medical Science, Professor, 総合医学研究所, 教授 (20139786)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Meth-A cells / V79 cells / tetraploid cell line / triploid cell line / octaploid cell line / polyploidization / demecolcine / fractal structure / polyloidization / K-252a / demecolcine |
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| Research Abstract |
This study aims to establish polyploid cells and to examine transformation in the cells. We have established polyploid Meth-A cell lines (4-, 6-, 8-, 10-, 12-, 16-and 18-ploid) and polyploid V79 cell lines (3-, 4-and 6-ploid). The transformation through polyploidization has been reported for the temperature dependence, serum concentration dependence and expression of cell-surface hydrocarbon chain.
This study intends to change a concept that chromosomes are independent each other in cells. I have made a model (genome-fractal structure model) based on the model that Manabu Takahashi (emeritus professor of Yamaguchi University) has introduced, and explained the experimental results using the model (Cell Prolif. 2002, 2003).
In order to enlighten about DNA structure in cells, I have published two books (the one is of translated book of Manabu Takahashi). A half of this RESEARCH RESULTS will be described for the genome-fractal structure model.
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