| Project/Area Number |
13670251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Nagasaki University |
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Principal Investigator |
AOKI Yoshiki Nagasaki University, Institute of Tropical Medicine, Professor, 熱帯医学研究所, 教授 (90039925)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Kanji Nagasaki University, Institute of Tropical Medicine, Research Associate, 熱帯医学研究所, 助手 (70325679)
FUJIMAKI Yasunori Nagasaki University, Institute of Tropical Medicine, Assistant Professor, 熱帯医学研究所, 講師 (10209083)
NIWA Masami Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (20136641)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | schistosome / miracidium / secretion of snail / chemo-klinokinesis / chemotaxis / lectin / WGA / cAMP / 感覚器 / ケモ・クリノカイネシス / 情報伝達 |
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| Research Abstract |
Schistosorne miracidia show chemo-klinokinesis toward the secretions of the intermediate snails (Snail Conditioned Water : SCW). A certain kinds of nematode larvae are reported to loose their chennotactic activity when their sensory papillae are overspread by some kinds of lectin. Miracidia must recognize SCW by the sensory papillae on their body surface. The present study was attempted to show the presence of receptor on the miracidia by using lectin binding. Materials and Method : Miracidia were incubated with any hind of lectin. They were then examined for their swimming behavior and for cheinotactic action toward SCW. Lectin used was WGA, PNA, GS-II and MPA. Results and Discussion : Of lectin examined, only WGA, at high concentration (100 ug/ml), caused the behavior, change (frequent change in swimming direction). And miracidia pre-incubated with WGA, at 250 ug/ml for 10 min, did not show the cherotactic action toward SCW. These results indicate that 1) the receptors of SCW are pre
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sent on the body surface of miracidia, and 2) miracidia loose chemotactic action when the receptors are overspread by WGA. Recently we reported that CAMP induces the frequent change in swimming direction of miracidia as well as SCW does. Then an attempt was made to examine whether WGA inhibits cAMP-induced miracidial behavior change. Miracidia pre-incubated with WGA showed the frequent change of swimming direction. Since this result suggests the role of cAMP as a 2^<nd> messenger of the signal transduction of chemo-klinokinesis of miracidia, an attempt was made to verify our hypothesis. Miracidia were incubated with the reagents that lower the intercellular e_AMP concentration (MDL-12,330A, SQ 22526, imidazole) or inhibitor of protein kinase A (PKI(14-22) amid), and then examined for their chemotactic action toward SCW. As was expected, these miracidia did reduce the chemotacitc activity toward SCW. These results clearly indicate that cAMP plays an important role in chemo-klinokinesis. of miracidia toward SCW. Less
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