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Identification of virulent factors related to rearrangement of skeleton Proteins in mycobacterial infections.

Research Project

Project/Area Number 13670289
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionResearch Institute, International Medical Center of Japan

Principal Investigator

KIRIKAE Teruo  International Medical Center of Japan Department of Tropical Medicine, Director, 研究所・感染・熱帯病研究部, 部長 (50192563)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsMycobacterium tuberculosis / Rhodococcus equi / Macrophage / TACO / ロドコッカク エクイ / BCG
Research Abstract

To identify new virulent factors of intracellular bacteria, such as Mycobacterium tuberculosis and Rhodococcus equi, murine macrophage-like J774. 1 cells were infected with virulent or avirulent R. equi and observed by using immunocytological methods. Observations by light microscopy and transmission electron microscopy showed that the virulent bacteria increased into single membrane vacuoles. Observations by confocal fluorescence microscopy with an endosomal marker and lysosomal markers revealed that most phagosomes containing virulent bacteria were fused with lysosomes. To monitor acidification of phagosomes containing R. equi, the cells infected with R. equi were stained with acidotropic probe. The phagosomes containing avirulent R. equi became acidic. In contrast, virulent R. eqiu did not change pH. The medium of virulent R. equi was suppressed acidification of acidic compartments in macrophages. These results suggested that virulent R. equi can survive phagolysosomal environment in macrophages by secreting substance which suppress acidification of phagolysosome.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Takahara, M., et al.: "Pulmonary Mycobacterium avium Infection an Immunocompetent Aged Woman Related to Use of Home Bath with a Ciruclating Water System"Jpn.J.Infect.Dis.. 55. 213-214 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Funatogawa, K., et al.: "Use of Immunoglobulin Enriched Bovine Colostrum against Oral Challenge with Enterohaemorrhagic Escherichia Coli O157:H7 in Mice"Microbiol.Immunol.. 46. 761-766 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takai, S., et aal.: "identification of virulence-associated antigens and plasmids in Rhodococcus equi from AIDS patients and prevalence of virulent R.equi in soil collected from domestic animal farms in Chiang Mai"Am.J.Trop.Med.Hyg.. 66. 52-55 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] hashimoto, H., et al.: "Structural study on lipid A and the O-specific polysaccharide of the lipopolysaccharide from a clinical isolate Bacteroides vulgatus from a patient with Crohn's disease"Eur.J.Biochem.. 269. 3715-3721 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahara, M., et al.: "Pulmonary Mycobacterium avium Infection in an Immunocompetent Aged Woman Related to Use of Home Bath with a Circulating Water System"Jpn. J. Infect. Dis.. 55. 213-214 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Funatogawa, K., et al.: "Use of Immunoglobulin Enriched Bovine Colostrum against Oral Challenge with Enterohaemorrhagic Escherichia coli O157:H7 in Mice"Microbiol. Immunol.. 46. 761-766 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takai, S., et al.: "Identification of virulence-associated antigens and plasmids in Rhodococcus equi from AIDS patients and prevalence of virulent R. equi in soil collected from domestic animal farms in Chiang Mai"Am. J. Trap. Med. Hyg.. 66. 52-55 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hashimoto, H., et al.: "Structural study on lipid A and the O-specific polysaccharide of the lipopolysaccharide from a clinical isolate Bacteroides vulgatus from a patient with Crohn's disease"Eur. J. Biochem.. 269. 3715-3721 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hashimoto, H., et al.: "Structural elucidation of capsular polysaccharide from a clinical isolate of Bacteroides vulgatus from a patient with Crohn's disease"Eur. J. Biochem.. 268. 1-7 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Watando, A. et al.: "Mycobacterium avium infection in an immunocompetent young adult related to use of home bath with a circulating water system"Jpn. J. Infect. Dis.. 54. 151-152 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hashimoto, H., et al.: "Structural study on lipid A and the 0-specific polysaccharide of the lipopolysaccharide from a clinical isolate Bacteroides vulgatus from a patient with Crohn's disease., 269:3715-3721, 2002."Eur. J. Biochem.. 269. 3715-3721 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahara, M., et al.: "Pulmonary Mycobacterium avium infection in an immunocompetent aged woman related to use of home bath with a circulating water system"Jpn. J. Infect. Dis.. 55. 213-214 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takai, S., et al.: "Identification of virulence-associated antigens and plasmids in Rhodococcus equi from AIDS patients and prevalence of virulent R. equi in soil collected from domestic animal farms in Chiang Mai"Trop. Med. Hyg. 66. 52-55 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hashimoto, H., et al.: "Structural elucidation of capsular polysacceharide from a clinical isolate of Bacteroides vulgatus from a patient with Crohn's disease."Eur.J.Biochem.. 268. 1-7 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Watando, A. et al.: "Mycobacterium avium infection in an immunocompetent young adult related to use of home bath with a circulating water system"Jpn.J.Infect.Dis.. 54. 151-152 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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