| Project/Area Number |
13670293
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | YAMAGATA UNIVERSITY |
|---|
Principal Investigator |
HONGO Seiji Yamagata University School of Medicine, Department of Bacteriology, Associate professor, 医学部, 教授 (90229245)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUGAWARA Kanetsu Yamagata University School of Medicine, Department of Bacteriology, Assistant, 医学部, 教務職員 (60110673)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | Influenza C virus / NS gene / NS2 / virion / incorporation |
|---|
| Research Abstract |
Recently we identified the 182 amino acid NS2 protein as a protein of 〜22kDa in influenza C virus-infected cells. In this study, it was demonstrated that the NS2 protein of influenza C virus was present in purified virions as follows.
1. Western blot analysis with anti-NS2 serum detected the NS2 protein in egg-grown influenza C/Yamagata/1/88 virions purified by centrifugation through a linear gradient of 5%-40% potassium tartrate. NS2 was also present either in purified virions of other strains grown in eggs or in purified C/Yamagata/1/88 virions grown in other host cells(MDCK or HMV-II).
2. The NS2 protein was detected in influenza C virions purified by using the binding activity either to the rat red blood cells or to anti-Hemagglutinin-esterase(HE) monoclonal antibody-conjugated protein A sepharose.
3. The NS2 protein associated with influenza C virions purified by centrifugation through a linear gradient of 5%-40% potassium tartrate was completely resistant to the treatment with 200 μg/ml of TPCK-trypsin for 30 min at 37℃.
4. These data indicate that the NS2 protein of influenza C virus is present inside the viral envelope.
|
