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Tumor immune escape and the role of CD4 regulatory T cells

Research Project

Project/Area Number 13670319
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

NAKAYAMA Eiichi  Okayama UniversityGraduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (60180428)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsimmune surveillance / regulatory T cells / murine tumors / tumor growth / CTL / methylcholanthrene / p53^<- / -> / CD25
Research Abstract

The involvement of two phenotypically different regulatory T cells in tumor growth was investigated. Treatment of BALB/c mice with anti-CD25 mAb (PC61), but not anti-CD4 mAb (GK1.5) before RL male 1 or Meth A inoculation caused the tumor rejection. On the other hand, treatment of BALB/c mice with anti-CD4 mAb (GK1.5) but not anti-CD25 mAb (PC61) on day 6 after inoculation of the same tumors caused rejection. The findings suggested that CD4^+CD25^+ T cells downregulated the rejection response in the early stage of tumor growth. On the other hand, CD4^+CD25^- T cells downregulated the tumor rejection response in the following stage. Both CD4^+CD25^+ and CD4^+CD25^- T cells appeared to inhibit the efficient generation of CTL. Treatment of the mice with anti-CD25 mAb (PC61) before tumor inoculation did not result in continuous tumor growth also suggested that CD4^+CD25^- regulatory T cells did not appear following CD25-depletion. The present study also demonstrated that the treatment of BALB/c mice with anti-CD25 mAb (PC61) at 4 or 6 weeks after 3-methylcholanthrene (3-MC) inoculation retarded tumor occurrence.
Furthermore, CD4^+CD25^+ regulatory cells appeared to be involved in tumorgenesis of p53^<-/-> mice. The findings indicated that CD4^+CD^25 regulatory cells are involved in tumorgenesis of established tumor and also tumorgenesis by methylcholanthrene and in p53^<-/-> mice.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Uenaka, A., et al.: "Cryptic cytotoxic T lymphocyte epitope on a murine sarcoma Meth A generated by exon extension as a novel machanism"J.Immunol.. 170. 4862-4868 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kuroki, M., et al.: "Repression of bleomycin-induced pneumopathy by tumor necrosis factor"J.Immunol.. 170. 567-574 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tawara, I., et al.: "Sequential involvement of two distinct CD4^+ regulatory T cells during the course of transplantable tumor growth and protection from 3-methylcholanthrene-induced tumorgenesis by CD25-depletion"Jpn.J.Cancer Res.. 93. 911-916 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sanda, W., et al.: "Immune responses against allogeneic and syngeneic tumors in aged C57BL/6 mice"Microbiol.Immunol.. 46. 513-529 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hata, H., et al.: "Occurrence of tumor antigen pRL1a specific CD8 T cells in spleen cells from syngeneic BALB/c semiallogeneic(BALB/c x C57BL/6)F_1 and allogeneic C57BL/6 mice"Int.J.Oncol.. 20. 1019-1025 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ota, S., et al.: "Cellular processing of a mulibranched lysine core with tumor antigen peptides and presentation of peptide epitopes recognized by cytotoxic T lymphocytes on antigen presenting cells"Cancer Res.. 62. 1471-1476 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Uenaka, A., et al.: "Cryptic cytotoxic T lymphocyte epitope on a murine sarcoma Meth A generated by exon extension as a novel mechanism"J.Immunol.. 170. 4862-4868 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kuroki, M., et al.: "Repression of bleomycin-induced pneumopathy by tumor necrosis factor"J.Immunol.. 170. 567-574 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tawara, I., et al.: "Sequential involvement of two distinct CD4^+ regulatory T cells during the course of transplantable tumor growth and protection from 3-methylcholanthrene-induced tumorgenesis by CD25-depletion"Jpn.J.Cancer Res.. 93. 911-916 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sanda, W., et al.: "Immune responses against allogeneic and syngeneic tumors in aged C57BL/6 mice"Microbiol. Immunol.. 46. 513-529 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hata, H., et al.: "Occurrence of tumor antigen pRL1a specific CD8 T cells in spleen cells from syngeneic BALB/c, semiallogeneic (BALB/c x C57BL/6)F_1 and allogeneic C57BL/6 mice"Int.J.Oncol.. 20. 1019-1025 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ota, S., et al.: "Cellular processing of a mulibranched lysine core with tumor antigen peptides and presentation of peptide epitopes recognized by cytotoxic T lymphocytes on antigen presenting cells"Cancer Res. 62. 1471-1476 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Uenaka, A., et al.: "Cryptic cytotoxic T lymphocyte epitope on a murine sarcoma Meth A generated by exon extension as a novel machanism"J. Immunol.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kuroki, M., et al.: "Repression of bleomycin-induced pneumopathy by tumor necrosis factor"J. Immunol.. 170. 567-574 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tawara, I., et al.: "Sequential involvement of two distinct CD4^+ regulatory T cells during the course of transplantable tumor growth and protection from 3-methylcholanthrene-induced trumorgenesis by CD25-depletion"Jpn. J. Cancer Res.. 93. 911-916 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sanda, W., et al.: "Immune responses against allogeneic and syngeneic tumors in aged C57BL/6 mice"Microbiol. Immunol.. 46. 513-529 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hata, H., et al.: "Occurrence of tumor antigen pRL1a specific CD8 T cells in spleen cells from syngeneic BALB/c, semiallogeneic (BALB/c x C57BL/6)F_1 and allogeneic C57BL/6 mice"Int. J. Oncol.. 20. 1019-1025 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ota, S., et al.: "Cellular processing of a mulibranched lysine core with tumor antigen peptides and presentation of peptide epitopes recognized by cytotoxic T lymphocytes on antigen presenting cells."Cancer Res.. 62. 1471-1476 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hata, H., et al.: "Occurrence of tumor antigen pRLla specific CD8 T cells from syngeneic in BALB/c, semiallogeneic(BALB/c x C57BL/6)F_1 and allogeneic C57BL/6 mice"Int.J.Oncol.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Ota, S., et al.: "Cellular processing of a multibranched lysine core with tumor antigen peptides and presentation of peptide epitopes recognized by cytotoxic T lymphocytes on antigen presenting cells"Cancer Res.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Uenaka, A., et al.: "ELISPOT cloning of tumor antigens recognized by cytotoxic T-lymphocytes from a cDNA expression library"Cancer Immunity. 1. 8-17 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kurashige, T., et al.: "NY-ESO-1 expression and immunogenicity associated with transitional cell carcinoma : correlation with tumor grade"Cancer Res.. 61. 4671-4674 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakamoto, N., et al.: "A novel Fc receptor for IgA and IgM is expressed on both hematopoietic and non-hematopoietic tissues"Eur.J.Immunol.. 31. 1310-1316 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ono, T., et al.: "Identification of proacrosin binding protein sp32 precursor as a human cancer/testis antigen"Proc.Natl.Acad.Sci.USA. 98. 3282-3287 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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