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Biological monitoring of trace chemicals in residential environments

Research Project

Project/Area Number 13670365
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionOkayama University

Principal Investigator

KIRA Shohei  Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (50033212)

Co-Investigator(Kenkyū-buntansha) SEKI Akihiko  Okayama University, Graduate School of Medicine and Dentistry, Instructor, 大学院・医歯学総合研究科, 助手 (20314685)
TAGUCHI Toyohiro  Kawasaki University of Medical Welfare, Professor, 医療福祉学部, 教授 (30197248)
KATAOKA Hiroyuki  Shujitu University, School of Pharmacy, Professor, 薬学部, 教授 (80127555)
WANG Da-hong  Okayama University, Graduate School of Medicine and Dentistry, Instructor, 大学院・医歯学総合研究科, 助手 (90294404)
YAMAMOTO Hideki  Okayama University, Graduate School of Medicine and Dentistry, Assistant Professor, 大学院・医歯学総合研究科, 講師 (50243457)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsBiological monitoring / Volatile organic compounds / Urinary metabolites / Xylene / Styrene / GC-MS / 生体試料 / フタル酸エステル / アルデヒド類 / ホルムアルデヒド / 固相マイクロ抽出法 / 高速液体クロマトグラフィー / 居住環境 / 化学物質 / 微量定量法
Research Abstract

objective : Sick house syndrome, the diverse health effects encountered by people dwelling or working in the new construction, is becoming a noticeable problem in Japan. This study was attempted to make a contribution toward prediction of the adverse health effects of chemicals in residential environments and planning preventive measures. This year we focused on developing a method for quantitative determination of trace level metabolites in urine of individuals exposed to aromatic volatile organic compounds (VOCs) such as toluene, xylene, and styrene by gas chromatography coupled with mass spectrometry (GC-MS).
Methods : We targeted of the urinary hippuric acid (HA), o-, m-, and p-methyl hippuric acids (o-, m-, p-MHA), and mandalic acid (MA) and phenylglyoxalic acid (PGA), metabolites of toluene, xylene, and styrene, respectively. Study samples were prepared by adding benzoylleucine as the internal standard (LS.), extracting with ethyl acetate under acid condition. The derivative compo … More unds were then reconstituted in ethyl acetate and identified by GC-MS equipped with DB-1 capillary column. Elution was carried out at initial column temperature 150℃ with an increment at a rate of 10℃/min up to 250℃. The quantitative analysis was operated in SIM mode, selecting the ion m/z 105 for HA, m/z 119 for MHA, m/z 107 for MA, and m/z 105 for PGA. In order to examine the corresponding concentrations of VOCs in ambient air, active sampling by charcoal tubes was used for VOCs collection. After elution with carbon disulfide, the samples were analyzed with GC-MS.
Results and discussion : Six urinary metabolites and I.S. were separated and eluted with a good peak by GC-MS. The limits of detection were 40-450 pg by SIM mode and the calibration curves of these compounds were fairly linear. Hippuric acid, MHA, MA, and PGA were all detected from urine of workers working in the new buildings in which higher levels of VOCs (toluene, xylene, and styrene) were found. Since HA of dietary sources could also affect its background level in urine, it cannot become a urinary marker of exposure to trace toluene. However, MHA, MA, and PGA can be used to evaluate low-level exposure to xylene and styrene.
Conclusions : This newly developed GC-MS method showed high sensitivity for determination of urinary metabolites of individuals exposed to trace level VOCs. The present results support its usefulness in biological monitoring of indoor VOCs for animals and humans. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Horike T.: "A simplifited procedure for the analysis of aldehydes by HPLC in aqueous and biological samples."10th International symposium on toxicity assessment abstracts. 33-34 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kira S.: "Monitoring of benzo(a)pyrene in sea water of the Seto Inland Sea, Japan."10th International symposium on toxicity assessment abstracts. 34-35 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 堀家 徳士: "ホルムアルデヒドの高感度分析法"産業衛生学雑誌. 44(2). 86-86 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroyuki Kataoka: "Automated on-line in-tube solid-phase microextraction coupled with high performance liquid chromatography for the analysis of bisphenol A, alkylphenols, and phthalate esters in foods contacted with plastics"Journal of Separation Science. 25(1-2). 77-85 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 瀧川 智子: "解剖実習におけるアルデヒド気中濃度"産業衛生学雑誌. 44巻臨時増刊号. 638-638 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大橋 泰浩: "新築建物における室内空気汚染分析と健康影響評価"第63回分析化学討論会講演要旨集. 150-150 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitani, K.: "Simple and rapid analysis of endocrine disruptors in liquid medicines and intravenous injection solutions by automated in-tube solid-phase microextraction/high performance liquid chromatography."Journal of Pharmaceutical and Biomedical Analysis. 32. 469-478 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大橋 泰浩: "揮発性有機化合物曝露による尿中代謝物の測定"日本分析化学会第52年会抄録集. 410-410 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 瀧川 智子: "パッシブサンプラーによる気中揮発性有機化合物の測定"日本衛生学雑誌. 59巻2号. 160-160 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Horike T.: "A simplifited procedure for the analysis of aldehydes by HPLC in aqueous and biological samples."10th International symposium on toxicity assessment abstracts. 33-34 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kira S.: "Monitoring of benzo(a)pyrene in sea water of the Seto Inland Sea, Japan."10th International symposium on toxicity assessment abstracts. 34-35 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Horike T.: "Highly sensitive method for formaldehyde measurement"Sangyo Eiseigaku Zasshi. 44(2). 86-86 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kataoka, H.: "Automated on-line in-tube solid-phase microextraction coupled with high performance liquid chromatography for the analysis of_bisphenol A, alkylphenols, and phthalate esters in foods contacted with plastics."Journal of Separation Science. 25(1-2),. 77-85 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takigawa, T.: "Ambient air formaldehyde level during practice in anatomy"Sangyo Eiseigaku Zasshi. 44. 638-638 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohashi Y.: "Indoor air pollution analysis and health effect evaluation in newly constructed buildings"63rd Bunseki Kagaku Touronkai Kouen Yousishu (Abstracts). 638-638 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitani, K.: "Simple and rapid analysis of endocrine disruptors in liquid medicines and intravenous injection solutions by automated in-tube solid-phase microextraction/high performance liquid chromatography."Journal of Pharmaceutical and Biomedical Analysis. 32. 469-478 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohashi, Y.: "Measurement for urinary metabolites of individuals exposed to volatile organic compounds"52nd Conference of the Japanese Society for Analytical Chemistry (Abstracts). 410-410 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takigawa, T.: "Measurement for ambient air volatile organic compounds by passive samplers"Japanese Journal of Hygiene. 59(2). 160-160 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大橋 泰浩: "揮発性有機化合物曝露による尿中代謝物の測定"日本分析化学会第52年会抄録集. 410-410 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 滝川 智子: "パッシブサンプラーによる気中揮発性有機化合物の測定"日本衛生学会雑誌. 59巻2号. 160-160 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiroyuki Kataoka: "Automated on-line in-tube solid-phase microextractia with high performance liquid chromatography for the analysis of bisphenol A, alkylphenols, and esters in foods contacted with plastics"Journal of Separation Science. 25(1-2). 77-85 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 瀧川 智子: "解剖実習におけるアルデヒド気中濃度"日本産業衛生学会雑誌. 44巻臨時増刊号. 638-638 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大橋 泰浩: "新築建物における室内空気汚染分析と健康影響評価"第63回分析化学討論会講演要旨集. 150-150 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Horike T.: "A simplifited procedure for the analysis of aldehydes by HPLC in aqueous and biological samples"10th International symposium on toxicity assessment abstracts. 33-34 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kira S.: "Monitoring of benzo (a) pyrene in sea water of the Seto Inland Sea, Japan"10th International symposium on toxicity assessment abstracts. 34-35 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 堀家 徳士: "ホルムアルデヒドの高感度分析法"第45回中国四国合同産業衛生学会抄録集. 82-83 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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