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Chromosome mapping of Y chromosomal STR DYS385 loci

Research Project

Project/Area Number 13670429
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research Institution宮崎医科大学

Principal Investigator

SEO Yasuhisa  Miyazaki Medical College, Faculty of Medicine, Associate Prof., 医学部, 助教授 (80187830)

Co-Investigator(Kenkyū-buntansha) TAKAMI Yasunari  Miyazaki Medical College, Faculty of Medicine, Associate Prof., 医学部, 助教授 (80236356)
KAKIZAKI Eiji  Miyazaki Medical College, Faculty of Medicine, Assistant Prof., 医学部, 助手 (70284833)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsLegal Medicine / Personal identification / DNA analysis / Y chromosome / STR locus / DYS385 locus / Haplotype / DNA多型 / STR / クロモゾームマッピング
Research Abstract

We developed a method for genotyping Y chromosome-linked homologous DYS385 loci individually, combining locus-specific PCR amplification and previously reported procedures. Duplicated DYS385a (5' -end) and DYS385b (3' ?end) loci were located about 41K bp apart and inverted to each other in the Y chromosome, which data was obtained from the human genome sequence in NCBI, and sequence differences were found at 424 bp down- and upstream of each locus. The locus-specific amplifications were performed using primers designed for this intergenic region, and fragments about 900 bp in length were produced. Polymorphic tetranucleotide arrays in the PCR products were typed according to procedures reported previously. Twenty male subjects were genotyped using this method. Alleles (GAAA)_13 to (GAAA)_21 were observed at the DYS385a locus, and those at DYS385b included alleles (GAAA)_9 to (GAAA)_15. DYS385a alleles, excluding those of identical arrays, were always larger than DYS385b alleles in the same subjects. These data suggest that the DYS385a and DYS385b loci can be amplified completely for discrimination, and the genotypes of the alleles provide information useful for forensic case work and population genetics.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 瀬尾 泰久: "DYS385の遺伝子型判定について"日本法医学雑誌. 55. 417 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuhisa Seo: "Genotyping of DYS385 locus"Jpn. J. legal Med.. 55. 417 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 瀬尾泰久: "DYS385の遺伝型判定について"日本法医学雑誌. 55・3. 417 (2001)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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