Establishment of analytical method for abused drugs by liquid chromatography/mass spectrometry
| Project/Area Number |
13670437
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Aichi Medical University |
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Principal Investigator |
SENO Hiroshi Aichi Medical University School of Medicine, Department of Legal Medicine, Professor, 医学部, 教授 (50236113)
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| Co-Investigator(Kenkyū-buntansha) |
HATTORI Hideki Aichi Medical University School of Medicine, Department of Legal Medicine, Associate Professor, 医学部, 助教授 (30107817)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Methamphetamine / Cocaine / Morphine / Codeine / High-performance liquid chromatography / Mass spectrometry |
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| Research Abstract |
Methamphetamine, morphine, codeine and cocaine were investigated by liquid chromatography (LC)/mass spectrometry (MS). Standard morphine, codeine and cocaine could be analyzed within 30 min with use of Shim-pack MAYI-ODS. Gradient elution was performed with mobile phase of 7.5 mM ammonium acetate and acetonitrile containing 0.05 mM formic acid. For methamphetamine, isolation from impurities was insufficient with the column; better separation had been achieved with other columns such as Mspak GF-310 4D. However, increase of the column pressure was less with Shim-pack than with Mspak, which meant that more samples could be analyzed continuously with Shim-pack.
For MS detection, electrospray ionization was employed. The highest sensitivity was obtained with the capillary temperature at 260℃; MS/MS gave 10 times higher sensitivity than MS.
Based on these results, we employed Shim-pack and MS/MS for identification of morphine, codeine and cocaine in human sera. Sufficient sensitivity such as 10-50 ng/ml was obtained with direct injection method without any extraction procedure. Actual identification of codeine was made for the blood obtained from an autopsy cadaver.
Direct injection method is very simple and rapid because it requires no complicated pre-treatment of samples. LC/MS with the combination of direct injection method and MS/MS seems very useful for the analysis of abused drugs.
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Report
(3 results)
Research Products
(3 results)
