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Identification of antigenic epitope for neutralizing antibody that inhibits enzymatic activity of Helicobacter pylori urease

Research Project

Project/Area Number 13670481
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionNippon Medical School

Principal Investigator

TAKAHASHI Hidemi  Nippon Medical School, Dept. of Microbiol. & Immunol., Professor and Chairman, 大学院・医学研究科, 教授 (40221361)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsHelicobacter pylori / urease / neutralizing antibody / epitope / MAP / mapping / vaccine / enzymatic activity / Helicobacter pylori / vaccine / Hybridoma / エピトープ / 合成ペプチド
Research Abstract

There may be two types of Helicobacter pylori (H.pylori) urease-specific antibodies, one is unfavorable to the body in progressing gastritis and the other is benefical in preventing bacterial growth and attachment to the gastric mucosa. To find the epitope for the latter type, a series of overlapping peptides were synthesized to identify the epitope of a mouse monoclonal antibody (mAb), termed L2, which has the capacity to generate complete suppression of the enzymatic activity of H. pylori urease. Among 79 overlapping peptides tested, a predominant epitope, UB-33 (aa 321-339; CHHLDKSIKEDVQFADSRI), was identified. Immunization with KLH-conjugated UB-33 induced UB-33-specific antibody which partially abrogated the urease activity. The minimal epitope of L2 was F8 (SIKEDVQF), whereas 6-mer peptide (KEDVQF) was the minimum for UB-33-specific rabbit sera. F8-based multiple antigenic peptides (MAP) generated UB-33-specific antibody that was much stronger than UB-33 but less potent than L2 and its minimal epitope was 6-mer (SIKEDV). The amino acid 329K seems to be critical to the L2-specific antibody response. The findings shown here provide important information on making a neutralizing vaccine to prevent H.pylori infection as well as its persistency in the stomach.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (87 results)

All Other

All Publications (87 results)

  • [Publications] Takahashi, M., Nakagawa, Y., Berzofsky, J.A., Takahashi, H.: "Counter-regulation of 1 cytolytic activity and cytokine production in human immunodeficiency virus(HIV)-1-specific murine CD8+ cytotoxic T lymphocytes by free antigenic peptide."Int.Immunol.. 13. 43-51 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakajima, Y., Takahashi, M., Norose, Y., Ogawa, R., Takahashi, H.: "Induction of apoptosis in mice thymocytes by tetracaine."Biomedical Res.. 21. 297-303 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watari, E., Shinya, E., Kurane, S., Takahashi, H.: "Effect of cyclosporin A on cell fusion in monkey kidney cell line persistently infected with measles virus."Intervirol.. 44. 209-211 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Machida, K., Kohara, K., Seike, E., Tone, S., Shibasaki, F., Shimizu, M., Takahashi, H., et al.: "Inhibition of cytochrome C release in Fas-mediated signaling path way by hepatitis C viral proteins in transgenic mice liver."J.Biol.Chem.. 276. 12140-12146 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirota, K., Nagata, K., Norose, Y., Futagami, S., Nakagawa, Y., Senpuku, H., Kobayashi, M., Takahashi, H.: "Identification of an antigenic epitope in Helicobacter pylori urease that induces neutralizing antibody production."Infect.Immu.. 69. 6597-6603 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamada, H., Hiroi, T., Nishiyama, Y., Takahashi, H., et al.: "Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine."J.Immunol.. 168. 57-64 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nisiyama, Y., Hamada, H., Nonaka, S., Yamamoto, H., Nanno, M., Katayama, Y., Takahashi, H, Ishikawa, H.: "Homeostatic regulation of intestinal villous epithelia by B lymphocytes."J.Immunol.. 168. 2626-2633 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yokosuka, T., Takase, K., Suzuki, M., Nakagawa, Y., Taki, S., Takahashi, H., Fujisawa, T., Arase, H., Saito, T.: "Predominant role of T cell receptor (TCR)-α chain in forming preimmune TCR repertoire revieled by clonal TCR reconstitution system."J.Exp.Med.. 195. 991-1001 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Enose, Y., Ui, M., Miyake, A., Suzuki, H., Uesaka, H., Kuwata, T., Kunisawa, J., Kiyono, H., Takahashi, H., et al.: "Protection by intranasal immunization of a nef-deleted, nonpathogenic SHIV against intravaginal challenge with a heterologous pathogenic SHIV."Virology. 298. 306-316 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, M., Osono, E., Nakagawa, Y., Wang, J., Berzofsky, J.A., Margulies, D.H., Takahashi, H.: "Rapid induction of apoptosis in CD8+ HIV-1 envelope-specific murine CTLs by short exposure to antigenic peptide."J.Immunol.. 169. 6588-6593 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichikawa, M., Sugita, M., Takahashi, M., Satomi, M., Takeshita, T., Araki, T., Takahashi, H.: "Brest milk macrophages spontaneously produce GM-CSF and differentiate into dendritic cells in the presence of exogenous IL-4 alone."Immunology. 108. 189-195 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaue, G., Hiroi, T., Nakagawa, Y., Someya, K., Iwatani, K., Sawa, Y., Takahashi, H., et al.: "HIV Mucosal Vaccine : Nasal Immunization with gp160-Encapsulated Hemagglutinating Virus of Japan-Liposome Induces Antigen-Specific CTLs and Neutralizing Antibody Responses."J.Immunol.. 170. 495-502 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takaku, S., Nakagawa, Y., Shimizu, M., Norose, Y., Maruyama, I., Takano, T., Kohara, M., Takahashi, H.: "Induction of hepatic injury by hepatitic C virus-specific CD8+ murine cytotoxic T lymphocytes in transgenic mice expressing the viral structural genes."Biochem.Biophys.Res.Commun.. 301. 330-337 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hidemi Takahashi: "Antigen presentation in vaccine development."Comp.Immunol.Microbiol.Infect.Dis.. 26. 309-328 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, J., Watari, E., Shinya, E., Norose, Y., Matsumoto, M., Seya, T., Sugita, M., Kawana, S., Takahashi, H.: "Down-regulation of Toll-like receptor expression in monocyte-derived Langerhans cell-like cells : implications of low-responsiveness to bacterial components in the epidermal Langerhans cells."Biochem.Biophys.Res.Commun.. 306. 674-679 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinya, E., Hidaka, C., Owaki, A., Shimizu, M., Watanabe, K., Watari, E., Klatzmann, D., Takahashi, H.: "Effect of Nef-deleted pseudotyeped HIV virions bearing an enhanced green fluorescent protein gene in the env on HIV-sensitive transformed T cells."Biomed.Res.. 24. 59-69 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim.Y., Nakagawa, Y., Sugiyama, H., Shimizu, M., Takeshita, T., Araki, T., Takahashi, H.: "Induction of CD4+ murine natural killer T-like cells by immunization with syngeneic thymoma expressing embryonic α-fetoprotein."Cell.Immunol.. 226. 1-10 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yanagihara, T., Kumagai, Y., Norose, Y., Moro, I., Nanno, N., Murakami, M., Takahashi, H.: "Age dependent decrease of polymeric Ig receptor expression and IgA elevation in ddY mice : a possible cause of IgA nephropathy."Lab.Invest.. 84. 63-70 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujimoto, C., Nakagawa, Y., Ohara, K., Takahashi, H.: "Polyriboinosinic polyribocytidylic acid (Poly(I : C))/TLR3 signaling allows class I processing of exogenous protein and induction of HIV-specific CD8+ CTLs."Int.Immunol.. 16. 55-63 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishii, R., Shimizu, M., Nakagawa, Y., Shimizu, K., Tanaka, S., Takahashi, H.: "In vivo priming of natural killer T cells by dendritic cells pulsed with hepatoma-derived acid-eluted substance."Cancer Immunol.Immunother.. 53. 383-390 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高橋秀実(分担): "免疫学イラストレイティッド"南江堂. 424 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高橋秀実(分担): "分子生物学・免疫学キーワード辞典"医学書院. 1035 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高橋秀実(監修): "ブラック微生物学"丸善出版. 866 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, M., Nakagawa, Y., Berzofsky, J.A., Takahashi, H.: "Counter-regulation of 1 cytolytic activity and cytokine production in human immunodeficiency virus (HIV)-1-specific murine CD8+ cytotoxic T lymphocytes by free antigenic peptide."Int.Immunol.. 13. 43-51 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakajima, Y., Takahashi, M., Norose, Y., Ogawa, R., Takahashi, H.: "Induction of apoptosis in mice thymocytes by tetracaine."Biomed.Res.. 21. 297-303 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watari, E., Shinya, E., Kurane, S., Takahashi, H.: "Effect of cyclosporin A on cell fusion in monkey kidney cell line persistently infected with measles virus."Intervirol.. 44. 209-211 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Machida, K., Kohara, K., Seike, E., Tone, S., Shibasaki, F., Shimizu, M., Takahashi, H., et al.: "Inhibition of cytochrome C release in Fas-mediated signaling pathway by hepatitis C viral proteins in transgenic mice liver."J.Biol.Chem.. 276. 12140-12146 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirota, K., Nagata, K., Norose, Y., Futagami, S., Nakagawa, Y., Senpuku, H., Kobayashi, M., Takahashi, H.: "Identification of an antigenic epitope in Helicobacter pylori urease that induces neutralizing antibody production."Infect.Immu.. 69. 6567-6603 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamada, H., Hiroi, T., Nishiyama, Y., Takahashi, Masunaga, Y., Hachimura, S., Kaminogawa, S., Takahashi-Iwanaga, H., Iwanaga, T., Kiyono, H., Yamamoto, H., Ishikawa, H.: "Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine."J.Immunol.. 168. 57-64 (2001, 2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishiyama, Y., Hamada, H., Nonaka, S., Yamamoto, H., Nanno, M., Katayama, Y., Takahashi, H., Ishikawa, H.: "Homeostatic regulation of intestinal villous epithelia by B lymphocytes."J.Immunol.. 168. 2626-2633 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yokosuka, T., Takase, K., Suzuki, M., Nakagawa, Y., Taki, S., Takahashi, H., Fujisawa, T., Arase, H., Saito, T.: "Predominant role of T cell receptor (TCR) -α chain in forming preimmune TCR repertoire revieled by clonal TCR reconstitution system."J.ExMed.. 195. 991-1001 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Enose, Y., Ui, M., Miyake, A., Suzuki, H., Uesaka, H., Kuwata, T., Kunisawa, J., Kiyono, H., Takahashi, H., Miura, T., Hayami, M.: "Protection by intranasal immunization of a nef-deleted nonpathogenic SHIV against intravaginal challenge with a heterologous pathogenic SHIV."Virology. 298. 306-316 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Li, Q., Nagahara, N., Takahashi, H., Takeda, K., Okumura, K., Minami, M.: "Organophosphorus pesticides markedly inhibit the activities of natural killer, cytotoxic T lymphocyte and lymphokine-activated killer : a proposed inhibiting mechanism via granzyme inhibition."Toxicology. 172. 181-190 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, M., Osono, E., Nakagawa, Y., Wang, J., Berzofsky, J.A., Margulies, D.H., Takahashi, H.: "Rapid induction of apoptosis in CD8^+ HIV-1 envelope-specific murine CTLs by short expsure to antigenic peptide."J.Immunol.. 169. 6588-6593 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichkawa, M., Sugita, M., Takahashi, M., Satomi, M., Takeshita, T., Araki, T., Takahashi, H.: "Brest milk macrophages spontaneously produce GM-CSF and differentiate into dendritic cells in the presence of exogenous IL-4 alone."Immunology. 108. 189-195 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaue, O., Hiroi, T., Nakagawa, Y., Someya, K., Iwatani, K., Sawa, Y., Takahashi, H., Honda, M., Kunisawa, J., Kiyono, H.: "HIV Mucosal Vaccine : Nasal Immunization with gp160-Encapsulated Hemagglutinating Virus of Japan-Liposome Induces Antigen-Specific CTLs and Neutralizing Antibody Responses."J.Immunol.. 170. 495-502 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takaku, S., Nakagawa, Y., Shimizu, M., Norose, Y., Maruyama, I., Wakita, T., Takano, T., Kohara, M., Takahashi, H.: "Induction of hepatic injury by hepatitic C virus-specific CD8+ murine cytotoxic T lymphocytes in transgenic mice expressing the viral structural genes."BBRC. 301. 330-337 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinya, E., Hidaka, C., Owaki, A., Shimizu, M., Li, Y., Watanabe, K., Watari, E., Hayami, M., Klatzmann, D., Takahashi, H.: "Effect of Nef-deleted pseudotyeped HIV virions bearing an enhanced green fluorescent protein gene in the env on HIV-sensitive transformed T cells."Biomed.Res.. 24. 59-69 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Narazaki, H., Watari, E., Shimizu, M., Owaki, A., Das, H., Fukunaga, Y., Takahashi, H., Sugita, M.: "Perfolin-dependent killing of tumor cells by VγIVδ1-bering T cells."Immuno.Lett.. 86. 113-119 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujimoto, C., Nakagawa, Y., Shimizu, M., Ohara, K., Takahashi, H.: "Isolation of antigenic substances from HIV-1 envelope gp120 gene transfectants by mild acid elution and X-irradiation treatment : for the development of CTL-based immunotherapy."Blamed.Res.. 24. 115-124 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawashima, T., Norose, Y., Watanabe, Y., Enomoto, Narazaki, H., Watari, E., Tanaka, S., Takahashi, H., Yano, I., Brenner, M., Sugita, M.: "Major CD8 T cell response to live bacillus Calmette-Guerin is madiated by CD1 molecules."J.Immunal.. 170. 5345-5348 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akahata, W., Ido, E., Akiyama, H., Uesaka, H., Enose, Y., Horiuchi, R., Kuwata, T., Goto, T., Takahashi, H., Hayami, M.: "DNA vaccination of macaques by a full-genome simian/human immunodeficiency virus type 1 plasmid chimera that produces non-infectious virus particles."J.Gen.Virol.. 84. 2237-2244 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, J., Watari, E., Shinya, E., Norose, Y., Matsumoto, M., Seya, T., Sugita, M., Kawana, S., Takahashi, H.: "Down-regulation of Toll-like receptor expression in monocyte-derived Langerhans cell-like cells : implications of low-responsiveness to bacterial components in the epidermal Langerhans cells."BBRC. 306. 674-679 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, H.: "Antigen presentation in vaccine development."CamImmunal.Micrabial. & Infect.. 26. 309-328 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim.Y., Nakagawa, Y., Sugiyama, H., Shimizu, M., Takeshita, T., Araki, T., Takahashi, H.: "Induction of CD4+ murine natural killer T-like cells by immunization with syngeneic thymoma expressing embryonic α-fetoprotein."Cell Immunol.. 226. 1-10 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yanagihara, T., Kumagai, Y., Norose, Y., Moro, I., Nanno, N., Murakami, M., Takahashi, H.: "Age dependent decrease of polymeric Ig receptor expression and IgA elevation in ddY mice : a possible cause of IgA nephropathy."Lab.Invest.. 84. 63-70 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujimoto, C., Nakagawa, Y., Ohara, K., Takahashi, H.: "Polyriboinosinic polyribocytidylic acid (Poly(I : C))/TLR3 signaling allows class I processing of exogenous protein and induction of HIV-specific CD8+ CTLs."Int.Immunol.. 16. 55-63 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishii, R., Shimizu, M., Nakagawa, Y., Shimizu, K., Tanaka, S., Takahashi, H.: "In vivo priming of natural killer T cells by dendritic cells pulsed with hepatoma-derived acid-eluted substance."Cancer Immunol. Immunother.. 53. 383-390 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, M., Nakagawa, Y., Berzofsky, J.A., Takahashi, H.: "Counter-regulation of 1cytolytic activity and cytokine production in human immunodeficiency virus (HIV)-1-specific murine CD8+ CTL free antigenic peptide."Int.Immunol.. 13. 43-51 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakajima, Y., Takahashi, M., Norose, Y., Ogawa, R., Takahashi, H.: "Induction of apoptosis in mice thymocytes by tetracaine."Biomed.Res.. 21. 297-303 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Watari, E., Shinya, E., Kurane, S., Takahashi, H.: "Effect of cyclosporin A on cell fusion in monkey kidney cell line persistently infected with measles virus."Intervirol.. 301. 209-211 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Machida, K., Takahashi, H., et al.: "Inhibition of cytochrome C release in Fas-mediated signaling pathway by hepatitis C viral proteins in transgenic mice liver."J.Biol.Chem.. 276. 12140-12146 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirota, K., Takahashi, H., et al.: "Identification of an antigenic epitope in Helicobacter pylori urease that induces neutralizing antibody production."Infect.Immu.. 69. 6597-6603 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujimoto, C., Nakagawa, Y., Shimizu, M., Ohara, K., Takahashi, H.: "Isolation of antigenic substances from HIV-1 envelope gp120 gene transfectants by mild acid elution and X-iirradiation treatment : for the development of CTL-based---"Biomed.Res.. 24. 115-124 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hamada, H., Hiroi, T., Nishiyama, Y., Takahashi, H., et al.: "Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine."J.Immunol.. 168. 57-64 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishiyama, Y., Takahashi, H., et al.: "Homeostatic regulation of intestinal villous epithelia by B lymphocytes."J.Immunol.. 168. 2626-2633 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yokosuka, T., Takahashi, H., et al.: "Predominant role of T cell receptor (TCR)-α chain in forming preimmune TCR repertoire revieled by clonal TCR reconstitution system."J.Exp.Med.. 195. 991-1001 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Enose, Y., Takahashi, H., et al.: "Protection by intranasal immunization of a nef-deleted, nonpathogenic SHIV against intravaginal challenge with a heterologous pathogenic SHIV."Virology. 298. 306-316 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Li, Q., Nagahara, N., Takahashi, H., Takeda, K., Okumura, K., Minami, M.: "Organophosphorus pesticides markedly inhibit the activities of natural killer, cytotoxic T lymphocyte and lymphokine-activated killer : a proposed inhibiting ---"Toxicology. 172. 181-190 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahashi, M., Osono, E., Nakagawa, Y., Takahashi., H.: "Rapid induction of apoptosis in CD8^+ HIV-1 envelope-specific murine CTLs short exposure to antigenic peptide."J.Immunol.. 169. 6588-6593 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ichikawa, M., Takahashi, H., et al.: "Brest milk macrophages spontaneously produce GM-CSF and differentiate into dendritic cells in the presence of exogenous IL-4 alone."Immunology. 108. 189-195 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakaue, G., Takahashi, H., et al.: "HIV Mucosal Vaccine : Nasal Immunization with gp160-Encapsulated Hemagglutinating Virus of Japan-Liposome Induces Antigen-Specific CTLs ---"J.Immunol.. 170. 495-502 (2003)

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      2003 Annual Research Report
  • [Publications] Takaku, S., Takahashi, H., et al.: "Induction of hepatic injury by hepatitic C virus-specific CD8+ murine cytotoxic T lymphocytes in transgenic mice expressing the viral structural genes."Biochem.Biophys.Res.Commun.. 301. 330-337 (2003)

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      2003 Annual Research Report
  • [Publications] Hidemi Takahashi: "Antigen presentation in vaccine development."Comp.Immunol.Microbiol.& Infect.Dis.. 26. 309-328 (2003)

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      2003 Annual Research Report
  • [Publications] Takeuchi, J., Takahashi, H., et al.: "Down-regulation of Toll-like receptor expression in monocyte-derived Langerhans cell-like cells : implications of low-responsiveness to bacterial components in the ---"Biochem.Biophys.Res.Commun.. 306. 674-679 (2003)

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      2003 Annual Research Report
  • [Publications] Kawashima, T., Takahashi, H., Brenner, M.B., Sugita, M., et al.: "Major CD8 T cell response to live bacillus Calmette-Guerin is madiated by CD1 molecules."J.Immunol.. 170. 5345-5348 (2003)

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      2003 Annual Research Report
  • [Publications] Shinya, E., Takahashi, H., et al.: "Effect of Nef-deleted pseudotyeped HIV virions bearing an enhanced green fluorescent protein gene in the env on HIV-sensitive transformed T cells."Biomed.Res.. 24. 59-69 (2003)

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      2003 Annual Research Report
  • [Publications] Akahata, W., Takahashi, H., et al.: "DNA vaccination of macaques by a full-genome simian/human immunodeficiency virus type 1 plasmid chimera that produces non-infectious virus particles."J.Gen.Virol.. 84. 2237-2244 (2003)

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      2003 Annual Research Report
  • [Publications] Kim Y., Takahashi, H., et al.: "Induction of CD4+ murine natural killer T-like cells by immunization with syngeneic thymoma expressing embryonic α-fetoprotein."Cell.Immunol.. 226. 1-10 (2003)

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      2003 Annual Research Report
  • [Publications] Yanagihara, T., Takahashi, H., et al.: "Age dependent decrease of polymeric Ig receptor expression and IgA elevation in ddY mice : a possible cause of IgA nephropathy."Lab.Invest.. 84. 63-70 (2004)

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      2003 Annual Research Report
  • [Publications] Fujimoto, C., Nakagawa, Y., Ohara, K., Takahashi, H.: "Polyriboinosinic polyribocytidylic acid (Poly(I : C))/TLR3 signaling allows class I processing of exogenous protein and induction of HIV-specific CD8+ CTLs."Int.Immunol.. 16. 55-63 (2004)

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      2003 Annual Research Report
  • [Publications] Ishii, R., Shimizu, M., Nakagawa, Y., Shimizu, K., Tanaka, S., Takahashi, H: "In vivo priming of natural killer T cells by dendritic cells pulsed with hepatoma-derived acid-eluted substance."Cancer Immunol.Immunother.. 53. 383-390 (2004)

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  • [Publications] 高橋秀実(分担): "免疫学イラストレイティッド"南江堂. 424 (2001)

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  • [Publications] 高橋秀実(分担): "分子生物学・免疫学キーワード辞典"医学書院. 1035 (2003)

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  • [Publications] 高橋秀実(監修): "ブラック微生物学"丸善出版. 866 (2003)

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  • [Publications] Hamada, H., Hiroi, T., Nishiyama, Y., Takahashi, H., et al.: "Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine"J. Immunol.. 168・1. 57-64 (2002)

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  • [Publications] Nishiyama, Y., Hamada, H., Nonaka, S., Takahashi, H., et al.: "Homeostatic regulation of intestinal villous epithelia by B lymphocytes"J. Immunol.. 168・3. 2626-2633 (2002)

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      2002 Annual Research Report
  • [Publications] Yokosuka, T., Takase, K., Suzuki, M., Nakagawa, Y., Taki, S., Takahashi, H., et al.: "Predominant role of T cell receptor (TCR)-a chain in forming preimmune TCR repertoire revieled by clonal TCR reconstitution system"J. Exp. Med.. 195・8. 991-1001 (2002)

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      2002 Annual Research Report
  • [Publications] Enose, Y., Ui, M., Miyake, A., Suzuki, H., Takahashi, H., et al.: "Protection by intranasal immunization of a nef-deleted, nonpathogenic SHIV against intravaginal challenge with a heterologous pathogenic SHIV"Virology. 298・2. 306-316 (2002)

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      2002 Annual Research Report
  • [Publications] Li, Q., Nagahara, N., Takahashi, H., et al.: "Organophosphorus pesticides markedly inhibit the activities of natural killer, cytotoxic T lymphocyte and lymphokine-activated killer : a proposed inhibiting mechanism via granzyme inhibition"Toxicology. 172・7. 181-190 (2002)

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      2002 Annual Research Report
  • [Publications] Takahashi, M., Osono, E., Nakagawa, Y., Wang, J., Berzofsky, J.A., Takahashi, H.: "Rapid induction of apoptosis in CD8+ HIV-1 envelope-specific murine CTLs by short exposure to antigenic peptide"J. Immunol.. 169・11. 6588-6593 (2002)

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      2002 Annual Research Report
  • [Publications] Takahashi, M., Nakagawa, Y., Berzofsky, J.A., Takahashi, H.: "Counter-regulation of cytolytic activity and cytokine production in human immunodeficiency virus (HIV)-1-specific murine CD8+ cytotoxic T lymphocytes by free antigenic peptide"Int. Immunol.. 13・1. 43-51 (2001)

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      2001 Annual Research Report
  • [Publications] Nakajima, Y., Takahashi, M., Norose, Y., Ogawa, R., Takahashi, H.: "Induction of apoptosis in mice thymocytes by tetracaine"Biomedical Res.. 21・5. 297-303 (2001)

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      2001 Annual Research Report
  • [Publications] Machida, K., Tsukiyama-Kohara, K., Seike, E., Tone, S., Shibasaki, F., Shimizu, M., Takahashi, H. et al.: "Inhibiton of cytochrome C release in Fas-mediated signaling pathway by hepatitis C viral proteins in transgenic mice liver"J. Biol. Chem.. 276・15. 12140-12146 (2001)

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      2001 Annual Research Report
  • [Publications] Watari, E., Shinya, E., Kurane, S., Takahashi, H.: "Effect of cyclosporin A on cell fusion in monkey kidney cell line persistently infected with measles virus"Intervirol.. 44・4. 209-211 (2001)

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      2001 Annual Research Report
  • [Publications] Hirota, K., Nagata, K., Norose, Y., Futagami, S., Nakagawa, Y., Senpuku, H., Kobayashi, M., Takahashi, H.: "Identification of an antigenic epitope in Helicobacter pylori urease that induces neutralizing antibody production"Infect. Immu.. 69・11. 6597-6603 (2001)

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      2001 Annual Research Report
  • [Publications] Hamada, H., Hiroi, T., Nishiyama, Y., Takahashi, H. et al.: "Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine"J. Immunol.. 168・1. 57-64 (2002)

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      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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