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Secretory Control and Intracellular Signal Transduction of Pancreatic Acinar Myofibroblast in Human

Research Project

Project/Area Number 13670509
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionShiga University of Medical Science

Principal Investigator

ISHIZUKA Yoshiyuki (2002)  Shiga University of Medical Science, Medical Science, Assistant, 医学部, 助手 (80303775)

新谷 寛 (2001)  滋賀医科大学, 医学部, 助手 (80303767)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordshPFCs / Matrix metalloproteinase-1 / Cytokines / Intracellular signal trancduction / MAPK / PKC / Northern blot / Zymography / Matrix metalloproteienase-1 / Nortern blot / ヒト膵腺房由来Myofibroblast / Matrix metalloproteinases / シグナル伝達 / protein kinase C / MAP kinase / RT-PCR / Northern blotting
Research Abstract

We confirmed that matrix metalloproteinases(MMPs, MMP-1, 2, 9) were secreted from human pancreatic periacinar fibroblast-like cells(hPFCs) by gelatin gel zymography. A little amount of MMPs secretion were continuously observed in the absent of stimulants and that was remarkably enhanced in the presence of pro-inflammatory cytokines like IL-1β and TNF-α. Enhanced secretion induced by an application of IL-1β and TNF-α were reduced by an application of apigenin, a MAPK inhibitor and PD98059, a MEK inhibitor in a dose dependent manner, but not PKC inhihitor and SB203580, a p38 MAPK inhibitor.
It was demonstrated that PKC-independent and MEK dependent signaling pathways induced by pro-inflammatory cytokines were closely concerned with MMP-producing system by a northern blot analysis. The inhibitors of NF-κB(PTDC, TPCK) had no effect on MMP-1 mRNA expression induced by an application of pro-inflammatory cytokines, therefore it was suggested that PDTC and TPCK not blockable transcriptional factors could be worked. Taken together, it resulted that pro-inflammatory cytokines stimulated MMPs production from hPFCs via some regulatory mechanisms involving MEK related signaling path ways.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 馬場忠雄, 五月女隆男, 新谷 寛: "ヒト膵腺房周囲筋線維芽細のmatrix metalloproteinase-1 (MMP-1)産生におけるシグナル伝達"厚生労働省特定疾患対策事業 難治性膵疾患に関する調査研究班 平成12年度研究報告書 平成13年3月発行. 228-232 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 新谷 寛, 馬場忠雄: "慢性膵炎 わが国の実態,成因と発症機序・進展機序"図説消化器病シリーズ14 膵炎,膵癌 平成13年1月. 84-98 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 馬場忠雄: "ヒト膵腺房周囲線維芽様細胞(hPFCs)におけるprotease-activated receptors (PARs)の存在と細胞増殖調節機構への関与"厚生労働省特定疾患対策事業 難治性膵疾患に関する調査研究班 平成13年度研究報告書 平成14年3月発行. 244-247 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 馬場忠雄, 五月女隆男, 新谷寛: "ヒト膵腺房周囲筋線維芽細胞のmatrix wetalloproteinase-1(MMP-1)産生におけるシグナル伝達"厚生労働省特定疾患対策事業難治性膵疾患に関する調査研究班平成12年度研究報告書. 228-232 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 新谷寛, 馬場忠雄: "慢性膵炎わが国の実態,成因と発症機序・進展機序"図説消火器病シリーズ14膵炎,膵癌. 84-98 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 馬場忠雄: "ヒト膵腺房周囲線維芽細胞(hPFC_2)におけるprotease-activated receptors(PARs)の存在と細胞増殖調節機構への関与"厚生労働省特定疾患対策事業難治性膵疾患に関する調査研究班平成13年度研究報告書. 244-247 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamura F, Shintani Y, Saotome T, et al.: "Effects of Serine Protease Inhibitors on Proliferation and Collagen Synthesis of Human Pancreatic Periacinar Fibroblast-like-Cells"Pancreas. 22. 317-325 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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