STUDY ON THE GENERATION MECHANISM OF INTRINSIC AUTO-FLUORESCENCE IN TUMOR TISSUES AND ITS APPLICATION TO THE EARLY DIAGNOSIS OF CANCER
| Project/Area Number |
13670559
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | JUNTENDO UNIVERSITY |
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Principal Investigator |
OGIHARA Tatsuo Juntendo University, Medical School, Associate Professor, 医学部, 助教授 (80011196)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Osamu Juntendo University, Medical School, Assistant Professor, 医学部, 講師 (80296871)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Auto-fluorescence / Fluorescence spectrum / Digestive tract / Tumor / Endoscope / Cancer diagnosis / Gastric cancer / Colonic cancer |
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| Research Abstract |
Research was made for the development of non-invasive visualization technique to obtain the pathological information of vital tissues. An autofluorescence endoscopic imaging system for gastrointestinal mucosa has been developed based on the concept that the autofluorescence spectra of neoplastic tissues are different from those of normal tissues when excited by blue light. This system consisted of an endoscope connected with high intensified cameras for the detection of weak autofluorescence image and a monitor on which a pseudo color image of neoplastic lesion is displayed in real time. We investigated the basic study and the clinical usefulness of the autofruorescence imaging system in the detection of neoplastic lesions. This system showed an ability in discriminating neoplastic tissues from normal tissues and small or superficial neoplastic lesions could be detected in colonic mucosa. In stomach, there were false positive cases in the autofluorescence diagnosis. The spectrum of aut
… More
o-fluorescence from gastrointestinal tumors was measured when excited by three wavelength light, blue, violet and ultraviolet. In adenocarcinoma of colon and well differentiated adenocarcinoma of stomach, blue light excitation induced better resolution in the tumor-specific autofluorescent spectra, while the excitation by violet and ultraviolet light induced better detection of tumorous lesions in poorly and undifferentiated adenocarcinomas of stomach. To analyze the factor determining the features of autofluorescence spectrum of cancerous tissues, the change in the autofluorescence image using the autofluorescence imaging system and the autofluorescence spectrum were observed on the tumor grown in nude mouse transplanted by cultured cell of colon and stomach cancer, HT29, HCA7, HCT116, DLD-land SW620. The spectral difference in autofluorescence between tumorous and non-tumorous tissues related to the difference in hemoglobin spectrum, which suggested that the tumor-specific autofluiorescence. might be caused by the specific distribution of blood volume of the tumor. Less
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Report
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Research Products
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