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Histological study of interstitial cells Cajal acting on the gastrointestinal motility.

Research Project

Project/Area Number 13670572
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionWaseda University

Principal Investigator

KOMURO Terumasa  KOMURO,Terumasa, 人間科学部, 教授 (20037386)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsICC / c-kit / Cornexin / Smooth muscle / Stomach / Immunohistochemistry / Ultrastructure / Enteric nerves / 消化管 / ペースメーカー / ICC / Kit / C-Kit / Gap junction / 免疫染色 / 蠕動運動 / c-Kit / 自律神経 / 蠕道運動
Research Abstract

This research project designed to elucidate the distribution and ultrastructure of different subtypes of interstitial cells of Cajal in the laboratory rodents.
2001〜 : Kit-immunohistochemistry demonstrated that in wild-type rats ICC were located at the submucosal border of the circular muscle layer (ICC-SM) in a extension of the gastric antrum, within both the circular (ICC-CM) and longitudinal (ICC-LM) muscle layers and in the myenteric plexus region (ICC-AP). In Ws/Ws rats, while ICC-CM and ICC-LM were not observed, a few ICC-SM and ICC-AP were found. The significance of their survival in the c-kit mutant animals is discussed. Ultrastructural observations also revealed synaptic structures between nerve terminals and both ICC-CM and ICC-LM, and between the nerve terminals and the muscle of both layers. It was concluded that the smooth muscle cells of the rat antrum are directly and indirectly innervated via ICC.
2002〜 : Reciprocal distribution of c-kit and Cx43 (gap junction protein) immunoreactivity within the mouse stomach was revealed. Regions with thick circular muscle layer in the cardia and fundus have a dense distribution of ICC-CM but only weak Cx43 immunoreactivity, whereas the thin muscle portion of the corpus was characterized by a sparse population of ICC-CM but a dense distribution of Cx43 immunoreactivity. These observations suggested that muscle cells of the cardia and fundus are not well coupled to each other by gap junctions and receive nerve signals via rich ICC-CM. In contrast, muscle at much fewer contacts with ICC-CM.
2003〜 : Three-dimensional constitution of each type of ICC network in the guinea-pig small intestine was clarified by using c-kit and PGP 9.5 immunohistochemistry to elucidate accurate relationships between ICC, the nerves and smooth muscle cells.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Mitsui R., Komuro T.: "Direct and indirect innervation of smooth muscle cells of rat stomach, with special reference to interstitial cells of Cajal."Cell Tissue Res.. 309. 219-227 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seki K., Komuro T.: "Distribution of interstitial cells of Cajal and gap junction protein, Cx 43 in the stomach of wild-type and W/W^V mutant mice."Anat. Embryol.. 206. 57-65 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsui R., Komuro T.: "Distribution and ultrastructure of interstitial cells of Cajal in the gastric antrum of wild-type and Ws/Ws rats."Anat. Embryol.. 206. 453-460 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Komuro T.: "Morphological features of interstitial cells of Cajal."Gann Monograph on Cancer Research, Gatrointestinal stromal tumor (GIST): from pathology to molecular target therapy. No52,(In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitusi R., Komuro T.: "Direct and indirect innervation of smooth muscle cells of rat stomach, with special reference to interstitial cells of Cajal."Cell Tissue Res.. 309. 219-227 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seki K., Komuro T.: "Distribution of interstitial cells of Cajal and gap junction protein, Cx 43 in the stomach of wild-type and W/W^v mutant mice."Anat.Embryol.. 206. 57-65 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsui r., Komuro t.: "Distribution and ultrastructure if interstitial cells of Cajal in the gastric antrum of wild-type and Ws/Ws rats."Anat.Embryol.. 206. 453-460 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Komuro T.: "Morphological features of interstitial cells of Cajal."Gann Monograph on Cancer Research, No52 Gatrointestinal stromal tumor (GIST) : from patholog to molecular target therapy.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsui R., Komuro T: "Distribution and ultrastructure of interstitial cells of Cajal in the gastric antrum of wild-type and Ws/Ws rats."Anat.Embryol.. 206:. 453-460 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Komuro, T.: "Morphological features of interstitial cells of Cajal."Gann Monograph on Cancer Research, No52, Gatrointestinal stromal tumor (GIST) : from pathology to molecular target therapy.. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ishikawa T., Nakayama S., Nakagawa T, Horiguchi K., Misawa H., Kadowaki M., Nakano A., Inoue S., Komuro T, Takaki M: "Characterization of in vitro gut-like organ formed from mouse embryonic stem cells."Am J Physiol Cell Physiol. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mitsui R., Komuro T.: "Direct and indirect innervation of smooth muscle cells of rat stomach, with special reference to the interstitial cells of Cajal"Cell and Tissue Res.. 309. 219-227 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Seki K., Komuro T.: "Distribution of interstitial cells of Cajal and gap junction protein, Cx43 in the stomach of wild-type and W/Wv mutant mice"Anat. Embryol.. 206. 57-65 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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