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Roles of type II alveolar epithelial cells in T cell-dependent immune response in Mycobacterium tuberculosis infection

Research Project

Project/Area Number 13670601
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionSHIMANE MEDICAL UNIVERSITY

Principal Investigator

SATO Katsumasa  SHIMANE MEDICAL UNIVERSITY, MICROBIOLOGY AND IMMUNOLOGY, INSTURUCTOR, 医学部, 助手 (00142331)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Toshiaki  SHIMANE MEDICAL UNIVERSITY, MICROBIOLOGY AND IMMUNOLOGY, INSTURUCTOR, 医学部, 助手 (60284030)
TOMIOKA Haruaki  SHIMANE MEDICAL UNIVERSITY, MICROBIOLOGY AND IMMUNOLOGY, PROFESSOR, 医学部, 教授 (40034045)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsM.tuberculosis / M.avium complex / type II alveolar pneumocytes / lymphocytes / macrophages / A-549 cells / PBMC / cytokines / M. avium complex / リンパ酸
Research Abstract

Our previous study revealed that both Mycobacterium tuberculosis (MTB) and M.avium complex (MAC) organisms invade type II alveolar epithelial cells in the lungs when mice are subjected to mycobacterial challenge via the intracheal route. In addition, MTB- or MAC-infected A-549 human type II alveolar epithelial cells (A-549 cells) release humoral factors (TNF-α and GM-CSF), thereby increasing antimycobacterial activity of macrophages(Mφs). In this study, the following findings have been obtained. (1) Anti-TNF-α and anti-GM-CSF antibodies attenuated A-549 cell-dependent inhibition of the intramacrophage growth of MTB and MAC, indicating crucial roles of these cytokines in A-549 cell-mediated potentiation of Mφ antimycobacterial activity. (2) When human peripheral blood mononuclear cells (PBMC) were added onto MTB-infected A-549 cells and Con A- or PPD-induced T cell mitogenesis was examined, T cell mitogenosis in the presence of A-549 cells was inhibited more profoundly than cases without A-549 cells. Such an inhibitory effect was also observed for A-549 cells without MTB infection. When a dual chamber system was used to prevent the cell contact between PBMC and A-549 cells, similar results were also obtained. Therefore, it is thought that the observed inhibition of PBMC mitogenesis due to A-549 cells is dependent upon some unknown humoral T cell-inhibitory factors released from A-549 cells.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Tomioka H: "Type II pneumocytes in the evaluation of drug antimycobacterial activity"Expert Opin Pharmacother. 4. 127-139 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato K, Tomioka H, Shimizu T, Gonda T, Ota F, Sano C: "Type II alveolar cells play roles in macrophage-mediated host innate resistance to pulmonary mycobacterial infections by producing proinflammatory cytokines"J Infect Dis. 185. 1139-1147 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sano K, Sato K, Sano C, Shimizu T, Tomioka H: "Comparative profiles of intramacrophage behavior of Mycobacterium tuberculosis and Mycobacterium avium complex with different levels of virulence"Microbiol Immunol. 46. 483-486 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogasawara K, Tomioka H, Shimizu T, Sano C, Kawauchi H, Sato K: "Profiles of cell-to-cell interaction of Mycobacterium intracellulare -induced immunosuppressive macrophages with target T cells in terms of suppressor signal transmission"Clin Exp Immunol. 129. 272-280 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tomioka H, Sato K, Shimizu T, Sano C: "Anti-Mycobacterium tuberculosis activities of new fluoroquinolones in combination with other antituberculous drugs"J Infection. 44. 160-165 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 佐藤勝昌, 冨岡治明, 清水利朗, 佐野千晶, 佐野啓介, 松島敏春: "マクロファージ内順化Mycobacterium tuberculosisおよびMycobacterium avium complexに対する各種抗菌薬の殺菌作用"日本化学療法学会雑誌. 50. 288-291 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 冨岡治明: "細菌学(竹田美文, 林 英生 編)、分担執筆"朝倉書店. 563-572 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tomioka H: "Type II pneumocytes in the evaluation of drug antimycobacterial activity"Expert Opin Pharmacother. 4. 127-139 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato K, Tomioka H, Shimizu T, Gonda T, Ota F, Sano C: "Type II alveolar cells play roles in macrophage-mediated host innate resistance to pulmonary mycobacterial infections by producing proinflammatory cytokines"J Infect Dis. 1139-1147 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato K, Tomioka H, Shimizu T, Gonda T, Ota F, Sano C: "Type II alveolar cells plays roles in macrophage-mediated host innate resistance to pulmonary mycobacterial infections by producing proinflammatory cytokines"J Infect Dis. 1139-1147 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogasawara K, Tomioka H, Shimizu T, Sano C, Kawauchi H, Sato K: "Profiles of cell-to-cell interaction of Mycobacterium intracellulare -induced immunosuppressive macrophages with target T cells in terms of suppressor signal transmission"Clin Exp Immunol. 129. 272-280 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tomioka H, Sato K, Shimizu T, Sano C: "Anti-Mycobacterium tuberculosis activities of new fluoroquinolones in combination with other antituberculous drugs"J Infection. 44. 160-165 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato K, Tomioka H, Shimizu, T Sano C, Sano K, Matsushima T: "Altered susceptibilities of Mycobacterium tuberculosis and Mycobacterium avium complex to rifalazil, clarithromycin, and levofloxacin after adaptation to intramacrophage milieus as determined by intracellular susceptibility testing"Jpn Society Chemother. 50. 288-291 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato K, Tomioka H, Shimizu T, Gonda T, Ota F, Sano C: "Type II alveolar cells plays roles in macrophage-mediated host innate resistance to pulmonary mycobacterial infections by producing proinflammatory cytokines"J Infect Dis. 185. 1139-1147 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sano K, Sato K, Sano C, Shimizu T, Tomioka H: "Comparative profiles of intramacrophage behavior of Mycobacterium tuberculosis and Mycobacterium avium complex with different levels of virulence"Microbiol Immunol. 46. 483-486 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ogasawara K, Tomioka H, Shimizu T, Sano C, Kawauchi H, Sato K: "Profiles of cell-to-cell interaction of Mycobacterium intracellulare-induced immunosuppressive macrophages with target T cells in terms of suppressor signal transmission"Clin Exp Immunol. 129. 272-280 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomioka H, Sato K, Shimizu T, Sano C: "Anti-Mycobacterium tuberculosis activities of new fluoroquinolones in combination with other antituberculous drugs"J Infection. 44. 160-165 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 清水利朗, 佐藤勝昌, 佐野千晶, 佐野啓介, 冨岡治明: "Antisense oligo DNAによる酸素依存性マクロファージ殺菌系および抗菌薬のMycobacterium avium complexに対する活性発現増強の試み"結核. 78. 33-35 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomioka H: "Type II pneumocytes in the evaluation of drug antimycobacterial activity"Expert Opin Pharmacother. 4. 127-139 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 冨岡治明: "細菌学(竹田美文,林 英生 編)、分担執筆"朝倉書店. 563-572 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤勝昌, 清水利朗, 冨岡治朗 他: "抗酸菌のマクロファージ(Mφ)と肺胞上皮細胞への感染性と細胞内増殖性:Mφ内順化菌を用いての検討"結核. 76. 53-57 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomioka H, Shimizu T, Sato K, et al.: "Expression profiles of low molecular mass heat shock proteins by Mycobacterium avium and Mycodacterium intracellulare"FEMS Microbiology Letters. 200. 131-136 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomioka H, Sato K, Shimizu T, et al.: "Intramacrophage passage of Mycobacterium tuberculosis and M.avium complex alters the drug susceptibility of the organisms as determineo by intracellular susceptibility testing using macrophages and type II alveolar epithelial cells"Antimicrobial Agents and Chemotherapy. 46. 519-521 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomioka H, Sato K, Shimizu T, et al.: "Anti-Mycobacterium tuberculosis activities of new fluoroquinolones in combination with other antituberculous drugs"Journal of Infection. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sato K, Tomioka T, Shimizu K, et al.: "Type II alveolar cells play roles in macrophage-mediated host innate resistance to pulmonary mycobacterial infections by producing proinflammatory cytokines"The Journal of Infectious Diseases. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 冨岡治朗: "結核(光山正雄 編)分担執筆"医薬ジャーナル社. 414 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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