• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The function of clara cells in lung remodeling induced by inhaled particles

Research Project

Project/Area Number 13670623
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

MORIMOTO Yasuo  UOEH, Institute of Industrial Ecological Sciences, Professor, 産業生態科学研究所, 教授 (30258628)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsClara cell / CGRP / CCSP / Lung injury / CGRP / マクロファージ / MMP
Research Abstract

In order to explore how CCSP or CGRP play a key role in lung injury and repair, we examined the function and expression of gene as follows using in vitro and in vivo studies.
1)epithelial injury induced by dust
Crystalline silica, chrysotile, Fe and man made mineral fibers (PT1),which we have used, were exposed in A549 cells within 48hours. These dusts induced apoptosis of alveolar epithelial cell through caspase activity and MAP kinase.
2)The effect of CGRP on proliferation of epithelial cells
CGRP stimulated proliferation of alveolar epithelial cells (A549 cells) through ERK
3)Gene expression of CGRP and CCSP in lung induced by dust.
Dusts (crystalline silica, crocidolite) with irreversible lung lesion decreased in gene expression of CCSP and CGRP in animal model, however dusts (PT1 and SiCW man-made mineral fibers) with reversible lesion increased in the gene expression.
4)Gene expression in clara cell-aberated mice induced crystalline silica
Clara cell-aberated mice were exposed to inhaled crystalline silica for 2 weeks. Clara cell inhibit gene expression of MMP, infiltration of macrophage and formation of foamy macrophage in the lung at lung injury induced by crystalline silica.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Kim, H-N: "Changes in DNA 8-hydroxyguanine Levels, 8-hydroxyguanine Repair Activity, and hOGG1 and hMTH1 mRNA Expression in Human Lung Alveolar Epithelial Cells Induced by Crocidolite Asbestos"Carcinogenesis. 22(2). 265-269 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ding, L.: "Gene Expression of Clara Cell Secretory Protein, Surfactant Protein-A and Thyroid Transcription Facter-1 in the Lungs of Rats Exposed to Patassium Octatitanate Whiskers in vivo"Journal of Occupational Health. 43(3). 111-117 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takatsu, H.: "Adachi, T ・Overexpression of EC-SOD supperesses endothelial-cell-mediated ldl oxidation"Biochem Biophys Res Commun. 285(1). 84-91 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yatera, K.: "Increased Expression of Matrix Metalloproteinase in Clara Cell-Ablated Mice Inhaling Crystalline silica"Environmental Health Perspectives. 109(8). 795-799 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morimoto, Y: "Expression of Clara Cell Secretory Protein in the Lungs of Rats Exposed to Silicon-carbide whisker in vivo"Toxicology letter. 145(3). 273-279 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morimoto, Y: "Gene Expression of Surfactant Protein-A and Thyroid Transcription Factor-1 in Lungs of Rats Exposed to Silicon-Carbide Whisker in vivo."J Occupational Health. 45. 307-312 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 森本 泰夫: "モデル動物の作成と維持 モデル動物各論"じん肺症モデル(in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim, H-N., Morimoto, Y., Tsuda, T., Ootsuyama, Y., Hirohashi, M., Hirano, T., Tanaka, I., Lim, Y., Yun, I-G, Kasai, H.: "Changes in DNA 8-hydroxyguanine Levels, 8-hydroxyguanine Repair Activity, and hOGG1 and hMTH1 mRNA Expression in Human Lung Alveolar Epithelial Cells Induced by Crocidolite Asbestos."Carcinogenesis. 22(2). 265-269 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ding, L., Morimoto, Y., Oyabu, T., Kim, H-N., Ogami, A., Yatera, K., Hirohashi, M., Yamato, H., Hori, H., Higashi, T., Tanaka, I.: "Gene Expression of Clara Cell Secretory Protein, Surfactant Protein-A and Thyroid Transcription Factor-1 in the Lungs of Rats Exposed to Potassium Octatitanate Whiskers in vivo"Journal of Occupational Health. 43(3). 111-117 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takatsu, H., Tasaki, Kim, H-N., Ueda, S., Tsutsui, M., Yamashita, K., Toyokawa, T., Morimoto, Y., Nakashima, Y., Adachi, T: "Overexpression of EC-SOD suppresses endothelial-cell-mediated ldl oxidation"Biochem Biophys Res Commun. 285(1). 84-91 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yatera, K., Morimoto, Y., Kim, H-N., Yamato, H., Tanaka, I., Kido, M.: "Increased Expression of Matrix Metalloproteinase in Clara Cell-Ablated Mice Inhaling Crystalline silica"Environmental Health Perspectives. 109(8). 795-799 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morimoto Y.: "Epithelial cells-Modifiers of lung injury"Journal of UOEH. 25(1). 259-267 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morimoto, Y., Ding, L., Oyabu, T., Hirohashi, M., Kim, H., Ogami, A., Yamato, H., Akiyama, I., Hori, H., Higashi, T., Tanaka, I.: "Gene Expression of Surfactant Protein-A and Thyroid Transcription Factor-1 in Lungs of Rats Exposed to Silicon-Carbide Whisker in vivo."J Occupational Health. 45. 307-312 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morimoto, Y., Ding, L., Oyabu, T., Hirohashi, M., Kim, H., Ogami, A., Yamato, H., Akiyama, I., Hori, H., Higashi, T., Tanaka, I.: "Expression of Clara Cell Secretory Protein in the Lungs of Rats Exposed to Silicon-carbide whisker in vivo"Toxicology letter. 145(3). 273-279 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morimoto, Y: "Gene Expression of Surfactant Protein-A and Thyroid Transcription Factor-1 in Lungs of Rats Exposed to Silicon-Carbide Whisker in vivo."J Occupational Health. 45. 307-312 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Morirmoto, Y: "Expression of Clara Cell Secretory Protein in the Lungs of Rats Exposed to Silicon-carbide whisker in vivo"Toxicology letter. 145(3). 273-279 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuoka, M: "Phosphorylation of p53 Protein in A549 Human Pulmonary pithelial Cells Exposed to Asbestos Fibers"Environmental Health Perspectives. 111(4). 509-512 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Morimoto, Y: "Epithelial cells-Modifiers of lung injury"Journal of UOEH. 25(sup). 259-267 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akiyama, I.: "Clearance of Deposited silicon Carbide Whisker from Rat Lungs Inhaled during a 4-Week Exposure"Journal of occupational Health. 45(1). 31-35 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 森本 泰夫: "ロックウールの人体に対する影響と作業環境の管理"日本医事新報. 4114. 116-117 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小畑 泰子, 小畑 英登, 森本 泰夫: "シュミレーション内科呼吸器疾患を探る-腫瘍編-"永井書店. 422 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 森本 泰夫: "ロックウールの人体に対する影響と作業環境の管理"日本医事新報. 4114. 116-117 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森本 泰夫: "肺のリモデリングにおけるクララ細胞の役割"Journal of UOEH. 24(2). 211-218 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森本 泰夫: "人造鉱物繊維の発がん性について-国際がん研究機関(IARC)の報告"産業医学ジャーナル. 25(3). 63-71 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamato, H: "Clearance of Inhaled Potassium Octatitanate Whisker from Rat Lungs"Journal of Occupational Health. 44. 34-39 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森本 泰夫: "国際ガン研究機関(IARC)のワーキンググループに参加して/IARC Working Group on the Evaluation of Carcinogenic Risks of Man-made Vitreous Fibers to Humans"エアロゾル研究. 17(1). 44-46 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morimoto, Y: "Combined effect of cigarette smoke (side stream) and mineral Fibers on the gene expression of cytokines mRNA in rat lungs"Journal of UOEH. 24. 32-37 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morimoto, Y: "IARC Monographs on the evaluation of carcinogenic risks to humans-Man-Made Vitreous Fibers-"IARC press. 422 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morimoto Y: "Comparison of gene expression of cytokines mRNA in lungs of rats induced by intratracheal instillation and inhalation of mineral fibers"Inhalation Toxicol. 13・4. 589-601 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ding L: "Gene expression of Clara cell secretory protein, Surfactant protein-A and Thyroid tanscription factor-1 in lungs of rats exposed to potassium octatitanate whisker in vivo"J. Occup. Health. 43. 111-117 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yatera K: "Increased expression of matrix metalloproteinase-2 and -9 in Clara cell-ablated mice exposed to inhaled crystalline silica"Environ Health Perspect. 109・8. 795-799 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kim H-N: "Changes in DNA 8-hydroxyguanine Levels, 8-hydroxyguanine Repair Activity, and hOGG1 and hMTH1 mRNA Expression in Human Lung Alveolar Epithelial Cells Induced by Crocidolite Asbestos"Carcinogenesis. 22・2. 265-269 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi