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Mechanisms of production of two new microglial growth-differentiation factors from brain astrocytes and neurons

Research Project

Project/Area Number 13670683
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionNational Center of Neurology and Psychiatry

Principal Investigator

KIKUCHI Aiko  National Center of Neurology and Psychiatry (NCNP), National Institute of Neuroscience, Dept. of Ultrastructural Res., Section Chief, 神経研究所・微細構造研究部, 室長 (70159010)

Co-Investigator(Kenkyū-buntansha) KAMO Isao  National Center of Neurology and Psychiatry (NCNP), National Institute of Neuroscience, Dept. of Ultrastructural Res., Section Chief, 神経研究所・微細構造研究部, 室長 (70108489)
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsmicroglia / neuron / astrocytee / thymic myoid cell / monocyte / 脳 / 胸腺 / 筋様細胞 / 胸腺筋様細胞 / 細胞増殖 / 細胞分化 / 神経
Research Abstract

Thymic myoid cells were found in many vertebrates. In particular, in human these cells are suggested to relate to autoimmune disease myasthenia gravis. We thought that these cells might have important roles in physiology. We succeeded in cloning myoid cells from the rat thymus. Indeed, these cells produce many cytokines, including an 80-kDa and 100-kDa haemopoietic factors. Both of them preferentially induce monocytic lineage cells. Using specific antibodies against these factors, we found that many myoid cells are increased in number and are producing these factors in the hyperplastic thymus associated with myasthenia gravis. Furthermore, we detected that these factors play important roles in specific proliferation of B-cells that recognized myoid cell s-own AChR. Brain also uses cytokines. Some of them are common to those used in the immune system. Expectedly, brain cells including neurons and glial cells produce the two factors. The purified two factors stimulate the growth and differentiation of microglial cells, suggesting that the new factors play important roles in diversification of monocytic lineage cells, together with macrophage colony stimulating factor and granulocyte-macrophage colony stimulating factor. Furthermore, these factors also stimulated MAPK of neurons, suggesting that they play important roles in neuron survival. We also succeeded in producing recombinant 80-kDa and 100-kDa factors.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Kikuchi, A., Kamo, I.: "Haemopoietic biglycan and IL-2 synergistically induce antigen-specific B-cell proliferation - A potential mechanism of anti-AChR antibody prodoution in the myasthenic thymus."Tohoku J.Exp.Med.. in press. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamo, I., Tomoyasu, H., Kikuchi, A.: "Cytokine field of hyperplastic thymus associated with myasthenia gravis"J.Neorological Sci.. 199. 95-95 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H., Iwakami, N., Fukushima, T., Kamo, I.: "Potential roles of two new haemopoietic factor in brain physiology"J.Neorological Sci.. 199. 95-95 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomoyasu, H., Kikuchi, A., Kamo I.: "Analysis of B-cell proliferation mechanism in the myasthenic hypertrophic thymus"Differentiation. 70. 375-375 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamo, I., Tomoyasu, H., Kikuchi, A.: "Analysis of B-cell proliferation mechanism in MG thymus hyperplasia"J.Neuroimmunol.. 118. 156-156 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi, A., Tomoyasu, H., Kamo I.: "Potential roles of 80-kDa and 100-kDa haemopoietic factor in brain physiology"J.Neuroimmunol.. 118. 28-28 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi A., Kamo I.: "Recent Reearch Development of Immunology"Haemopoietic biglycan. 5. 85-96 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi, A., Kamo, I.: "Analysis of B-cell proliferation mechanism in MG thymus hyperplasia"J.Neuroimmunol.. 118. 156-156 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi, A., Tomoyasu, H., Kamo, I.: "Potential roles of 80-kDa and 100-kDa haemopoietic factor in brain physiology,"J.Neuroimmunol.. 118. 28-28 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomoyasu, H., Kikuchi, A., Kamo, I.: "Immunohistochemical study of cytokines in the MG thymus hyperplasia,"J.Neuroimmunol.. 118. 161-161 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamo, I., Tomoyasu, H., Kikuchi, A.: "Cytokine field of hyperplastic thymus associated with myasthenia gravis,"J. Neurological Sci.. 199. s95 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi, A., Kikuchi, T., Tomoyasu, H., Iwakami, N., Fukushima, T., Kamo, I.: "Potential roles of two new haemopoietic factor in brain physiology,"J. Neurological Sci.. 199. s95 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomoyasu, H., Kikuchi, A., Kamo, I.: "Analysis of B-cell proliferation mechanism in the myasthenic hypertrophic thymus,"Differentiation. 70. 375-375 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi, A., Kamo, I.: "Haemopoietic biglycan and IL-2 synergistically induce antigen-specific B-cell proliferation -A potential mechanism of anti-AChR antibody production in the myasthenic thymus."Tohoku J.Exp.Med.. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi A., Kamo I.: "Haemopoietic biglycan,"Recent Res.Devel.Immunol.. 5. 85-96 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi, A., Kamo, I.: "Haemopoietic biglycan"Recent Res.Develop.Immunology. 5. 85-96 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kikuchi, A., Kamo, I.: "Pleiotropy of novel 80-kDa and 100-kDa haemopoietic facotor"生化学. 75. 21-21 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kikuchi, A., Tomoyasu, H., Kamo, I.: "Roles of 80-kDa and 100-kDa haemopoietic factor in brain"Brain Pathology. 15. 71-71 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kikuchi, A., Tomoyasu, H., Kamo, I.: "Neural and microglial stimulatory roles of two new heamopietic factors"Cell biology of the Neuron. 15. 51-51 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 柿沼 健一, 田部 美穂, 横山 安伸, 荻野 郁子, 阿部裕介, 星田 修, 新井一, 菊池 愛子, 加茂功, 桜川 宣男: "ヒト羊膜由来幹細胞(SP細胞)の酵素活性とその脳移植治療研究"Japanese Journal of Inherited Metabolic Diseases. 19. 167-167 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 加茂 功, 軍司健, 小坂 真一朗, 小林護, 佐々木裕子, 桜川宣男, 菊池愛子: "胸腺由来多分化能細胞の研究"Regenerative Medicne. 3. 126-126 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kikuchi, A., Kikuchi, T., Tomoyasu, H., Iwakami N., Fukushima, T.: "Analysis of B-cell proliferation mechanism in the myasthenic hypertrophic thymus, Differentiation"J. Neurological Sci.. 199. S95 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kamo, I., Tomoyasu, H., Kikuchi, A.: "Cytokine field of hyperplastic thymus associated with myasthenia gravis,"J. Neurological Sci.. 199. S95 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomoyasu, H., Kikuchi, A., Kamo, I.: "Analysis of B-cell proliferation mechanism in the myasthenic hypertrophic thymus,"Differentiation. 375-375 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kikuchi, A., Kamo, I.: "Haemopoietic biglycan and IL-2 synergistically induce antigen-specific B-cell proliferation-A potential mechanisam of anti-AChR antibody prodcution in the"Tohoku J. Exp. Med.. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kikuchi A., Kamo I.: "Cytokine field of hyperplastic thymus asociated with myashenia gravis 10^<th> International Congress of Neuromuscular Diseases. July 11 2002 Vancouver"Pandali Research Sinppos. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kikuchi A., Tomoyasu H., Kamo I.: "Potential roles of 80-kDa and 100-kDa haemopoietic factor in brain physiology"Journal of Neuroimmunology. 118. 28-28 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kamo I., Tomoyasu H., Kikuchi A.: "Analusis of B-cell proligeration mechanism in MG thymus hyperplasia"Journal of Neuroimmunology. 118. 156-156 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomoyasu H., Kikuchi A., Kamo I.: "Immunological study of cytokines in the MG thymus hyperplasia"Journal of Neuroimmunology. 118. 161-161 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 友安 浩, 河野 匡, 谷村繁雄, 菊池愛子, 加茂 功: "重症筋無力症胸腺内抗アセチルコリンレセプター抗体産生機構に関する研究"The Journal of Throcic and Cardiovascular Surgery. 49. 201-201 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 菊池愛子, 加茂 功: "新規80-kDa造血因子の多様性・共同作用に関する研究"生化学. 73. 1075-1075 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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