| Project/Area Number |
13670699
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
MINATOGUCHI Shinya Graduate School of Medicine, Associate Professor, 大学院・医学研究科, 助教授 (20190697)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Hisayoshi Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (80115930)
KOSAI Kenichiro School of Medicine, Assosiate Professor, 医学部, 助教授 (90258418)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | sFas gene therapy / myocardial infarction / LV remodeling / heart failure / Fas / Fas Ligand system / Fasリガンド・システム |
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| Research Abstract |
We examined potential therapeutic effects of soluble Fas (sFas), an inhibitor of apoptosis, on post-infarct left ventricular remodeling and heart failure. On the 3rd day of myocardial infarction (MI) of mice, adenovirus encoding sFas (Ad.CAG-sFas) was injected into the hindlimb muscle (1x10^9 pfu/mouse) to deliver sFas gene. As a control, adenovirus encoding LacZ gene was used. The treatment with sFas gene successfully suppressed apoptosis of granulation tissue cells, resulting in "a cell-rich scar tissue" at chronic stage (4 weeks later) in which blood vessels and myofibroblasts were abundant among fibrous tissue. The treatment greatly alleviated post-infarct left ventricular remodeling (based on improved echocardiogram and reduced heart to body weight ratio) and dysfunction (based on hemodynamic measurements). The results may imply a new therapeutic strategy against post-infarct heart failure, which is applicable even at subacute stage of MI.
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