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Functional analysis of atypical OKC and ASIP in cardiovascular diseases

Research Project

Project/Area Number 13670733
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionYokohama City University School of Medicine

Principal Investigator

UMEMURA Satoshi  Yokohama City University School of Medicine, Dept. of Medicine II, Yokohama City Univ. Sch. Of Med., Professor, 第二内科学教室, 教授 (00128589)

Co-Investigator(Kenkyū-buntansha) SUZUKI Atsushi  Yokohama City University School of Medicine, Dept. of Molecular Biology, Yokohama City Univ. Sch. Of Med., Assistant Professor, 第二生化学教室, 講師 (00264606)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsPKC / atypical PKC / ASIP / Signal Transduction / Hypertrophy / Heart Failure / Growth Factor / Cardiomyocyte
Research Abstract

Protein Kinase C (PKC) signaling pathway is involved in a variety of biological functions. Since activation of PKC pathway has been implicated in the development of cardiomyocyte hypertrophy, inhibition of this pathway would be an attractive target for the suppression of cardiomyocyte hypertrophy. Previous reports suggest that conventional PKC and novel PKC cause hypertrophic responses. We have recently focused our studies of PKC on atypical PKC, the third class of PKC family which is TPA and Ca2+insensitive and atypical PKC specific interacting protein (ASIP).
EGF are known to induce hypertrophic responses in cardiomyocytes, and by utilizing two PKC inhibitors showing species-dependent specificity, we suggested the possible involvement of atypical PKC in EGF signaling in the heart. We examined the cardiac expression of individual PKC isoforms at the cardiac hypertrophy stage and the heart failure stage in Dahl salt-sensitive rats by Western blot analysis. The *xpressions of PKCα, β and δ increased at the cardiac hypertrophy stage and remained the elevated at the heart failure stage. On the other hand, the expression of PKC_ε and aPKCs increased at the cardiac hypertrophy stage, but this increase tended to decline at the congestive heart failure stage. These results suggest that there are two groups of PKC isoforms. Several reports suggest that PKC_α, β and δ are involved in the development of cardiac hypertrophy and heart failure and that PKC_ε plays a role in the physiological hypertrophic response and cardioprotective actions.
These facts might indicate that all PKC isoforms (PKCα, β, δ, ε and aPKCs) expressed in the heart had similar function at the cardiac hypertrophy stage but two groups of PKC isoforms (PKCα, β, δand PKC_ε, aPKCs) had different function at the congestive heart failure stage.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Y.Koide, T.Tamura, A.Suzuki, S.Ohno, S.Umemura: "Differencial induction of PKC isoforms at the cardiac hypertrophy stage and congestive failure stage"Hypertension Research. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Ohkusu, N.Takahashi, K.Uchino, T.Inoue, S.Umemura: "Quantitative assessment of the total myocardial uptake ratio of 123I-BMIPP by using the Ishii-MacIntyre method is useful for MERAS"Nuclear Medicine Communications. Vol.24, No.2. 183-190 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Hanada, O.Tochikubo, K.Kimura, S.Umemura: "Estimation of myocardial cell damage on the basis mean electrocardiografic voltage and anatomical left ventrisular mass"Hypertension Research. Vol.25, No.1. 19-24 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Ebina, N.Takahashi, I.Mitani, T.Ishigami, T.Inoue, S.Umemura: "Clinical implications of cardiac 123I-meta-iodobenzylguanidine scintigraphy and cardiac natriuretic peptides in patients with heart disease"Annual Nuclear Medicine. Vol.23, No.8. 795-801 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Wada, S.Ueda, N.Kuji, K.Sugimoto, S.Umemura: "Angiotensin-II attenuates the vasodilationg effect of a nitric oxide donor, glyceryl trinitrate : roles of super oxide and angiotensin-II type I receptors"Clinical Pharmacology and Theraupetics. Vol.71, No.6. 440-447 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Fujita, Y.Toya, K.Iwatsubo, Y.Ishikawa, S.Umemura: "Accumulation of molecules involved in alpha 1-adrenergic signal within caveolae : caveolin expression and the development of cardiac hypertrophy"Cardiovascular Research. Vol.51, No.4. 709-716 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 仲澤 一郎, 梅村 敏: "テーラーメイド医療の展望 高血圧症"株式会社日本臨床. 7 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yuichi KOIDE, Kouichi TAMURA, Atsushi SUZUKI, Kouichi KITAMURA, Keiko YOKOYAMA, Tasuo HASHIMOTO, Nobuhito HIRAWA,Minoru KIHARA, Shigeo OHNO and Satoshi UMEHARA: "Different induction of PKC isoforms at the cardiac hypertrophy stage and congestive heart failure stage in Dahl salt-sensitve rats."Hypertension Research. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Koide, T.Tamura, A.Suzuki, S.Ono, S.Umemura: "Differencial induction of PKC isoforms at the cardiac hypertrophy stage and congestive failure stage"Hypertension Research. (In Press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Ohkusu, N.Takahashi, K.Uchino, T.Inoue, S.Umemura: "Quantitative assessment of the total myocardial uptake ratio of 123I-BMIPP by using the Ishii-MacIntyre method is useful for MERAS"Nuclear Medicine Communications. Vol.24,No.2. 183-190 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Ohkusu, N.Takahashi, K.Uchino, T.Inoue, S.Umemura: "Anti-tachycardia therapy can improve altered cardiac adrenergic function in tachycardia induced cardiomyopathy"Annual Nuclear Medicine. Vol.16,No.7. 447-453 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Ebina, N.Takahashi, I.Mitani, T.Ishigami, T.Inoue, S.Umemura: "Clinical implications of cardiac 123I-meta-iodobenzylguanidine scintigraphy and cardiac natriuretic peptides in patients with heart disease"Annual Nuclear Medicine. Vol.23,No.8. 795-801 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Wada, S.Ueda, N.Kuji, K.Sugimoto, S.Umemura: "Angiotensin-II attenuates the vasodilationg effect of a nitric oxide donor, glyceryl trinitrate : roles of super oxide and angiotensin-II type I receptors"Clinical Pharmacology and Theraupetics. Vol.71,No.6. 440-447 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] G.Yasuda, K.Shibata, T.Takizawa, Y.Ikeda, S.Umemura: "Prevalence of constipation in continuous ambulatory peritoneal dialysis patients and comparison with hemodialysis patients"American Journal of Kidney Disease. Vol.39,No.6. 1292-1292 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 石川 利之, 梅村 敏: "日本臨床 房室ブロック/洞不全症候群"株式会社日本臨床. 7 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Hanada, O.Tochikubo, T.Shigemasa.K.Kimura, S.Umemura: "Estimation of myocardial cell damage on basis of electrocardiographic voltage and anatomical left ventricular mass"Hypertension Research. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Fujita, Y.Toya, K.Iwamoto, K.Kimura, S.Umemura, Y.Ishikawa: "Accumulation of molecules involved in alfa1-adrenerc signal within caveolae : caveolin expression and the development of cardiac hypertrophy"Cardiovascular Research. Vol.51 No.4. 709-716 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] N.Hirawa, Y.Uehara, M.Kihara, Y.Toya, M.Takagi, S.Umemura: "Lidocalin-Type prostaglandin D synthase in essentioal hypertension"Hypertension. Vol.39 No.2. 449-454 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Ueda, SMaemoto, A.Wada, M.Ishii, KB Brosnihan, S.Umemura: "Angiotensin-(1-F7) potentiates bradykinin-induced vasodilatation in man"Journal of Hypertension. Vol.19 No.11. 2001-2009 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Mori, H.Kitahara, QH.Song, K.Miyamoto, S.Umemura, J.C.Cyong: "A new murine model for atherosclerosis with inflammation in the periodontal tissue induced by immunization with heat shock protein 60"Hypertension Research. Vol.23 No.5. 475-481 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Umemura: "Randamized controlled trials on hypertension"Japan Medical Association Journal. Vol.44 No.10. 461-468 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 仲澤 一郎, 梅村 敏: "日本臨床 テーラーメイド医療の展望 高血圧症"株式会社日本臨床. 9 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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